Based on the knowledge of heme bio-synthetic and metabolic pathway structures biliverdin protoporphyrin, experiments were carried out to compare difference between total quality eggshell pigments in blue-shelled eggs brown-shelled from same population (Dongxiang, China) analyze correlation quantity protoporphyrin 2 kinds eggshells. It was found that there no significant Dongxiang (P = 0.9006), a highly positive blue eggshells < 0.01) brown 0.05). These results suggested probably derived...

Crown gall induced by Agrobacterium vitis is a worldwide plant disease in grape-growing regions. Rahnella aquatilis HX2, new isolate from vineyard soil Beijing, showed significant inhibition effect on the development of crown galls grapevines. In field trials, immersion basal ends grape cuttings with HX2 cell suspension inhibited or completely prevented formation caused A. K308 roots plants cuttings. The 3-year average incidence treated was 30.8% compared to 93.5% without HX2. culture...

The objective of the present study was to evaluate role myofiber characteristics and thickness 2 major muscle membranes, perimysium endomysium, in determining breast meat tenderness chickens. Birds from breeds (White Leghorn a line broiler) were chosen. Chicks sexed wing-banded at hatch grown separate cages single house. Sixty broilers 60 White Leghorns harvested 6 wk age, respectively, whereas another slaughtered 18 age. An equal number males females maintained for each group. Body weight,...

Five hundred and four 40-wk-old Brown Dwarf hens (1.51 +/- 0.08 kg BW) were fed corn-soybean meal diets containing 0, 1, 2, 3, 4, 5, or 6% conjugated linoleic acid (CLA) for 56 d to measure the effects of dietary CLA on laying hen productivity egg quality during refrigerated storage. Four placed in 1 cage, 3 cages grouped as replicate resulting 6 replicates per treatment. After feeding experimental 11 d, eggs collected determine fatty composition yolks. From 12 18, from stored at 4 degrees C...

The peroxisome proliferators-activated receptor-γ coactivator-1α (PGC-1α) was investigated as a candidate gene for growth and fatness traits in chicken because of its prominent role muscle fiber specialization adipogenesis. A single nucleotide polymorphism (SNP) from G to at position 646 the open reading frame PGC-1α causing an Asp216Asn amino acid substitution identified. frequencies alleles genotypes were significantly different among 6 breeds (P < 0.01). White Plymouth Rock had highest...

Monocytes-macrophages are crucial players in specific and nonspecific immune responses to protect organisms from invasion of bacteria or viruses. In this study, monocytes circulation 2 lines Silky Starbro chickens with different disease resistance were separated cultured vitro. After identification acridine orange (AO), Giemsa staining, CD14 immunostaining, monocytes-macrophages used for adherence phagocytosis test. The overall percentages was 1.5 times greater than that (P < 0.01), which...

Chicken is considered to be an excellent model for genetic studies of phenotypic and genomic evolution, with large effective population size, specialized commercial lines, strong human-driven selection. High-density chicken SNP chips can help achieve a better understanding the selection mechanisms in artificially selected populations. We performed genome-wide tests signature 385 White Leghorn hens mapped positively regions genome annotations. Ten QTL related egg production, quality, growth,...

NADH dehydrogenase (complex I) catalyzes the transfer of electrons from to ubiquinone with pumping protons across mitochondrial inner membrane and produces reactive oxygen species as a major source in mitochondria. A missense mutation mitochondrially encoded 2 (MT-ND2) gene, which could produce change protein's secondary structure, has been found Tibet chicken breed. In this study, breeding eggs breed two genotypes were divided into groups. One group was incubated normoxia (20.9%...

We aimed to explore the effects of two different doses Zn on fecal microbiota in pigeons and correlation between these intestinal immune status. affected pigeon growth performance, T60 (60 mg/kg Zn) T90 (90 groups exhibited higher villus height crypt depth duodenum ileum compared control group, respectively. Supplementation with increased expression

Some members of the low-density lipoprotein receptor (LDLR) family play important roles in regulation metabolism and egg quality traits. Low-density receptor-related protein 2 (LRP2) gene belongs to LDLR super family, widely expresses many tissues. This work identified genotyped 1 single-nucleotide polymorphism (SNP), T14347C, at 3'-UTR LRP2 using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS), analyzed effects SNP (T14347C) on egg-quality traits...

Wolbachia can modulate the reproductive development of their hosts in multiple modes, and cytoplasmic incompatibility (CI) is most well-studied phenotype. The whitefly Bemisia tabaci highly receptive to different strains: wCcep strain from rice moth Corcyra cephalonica wMel fruit fly Drosophila melanogaster could successfully establish induce CI transinfected whiteflies. Nevertheless, it unknown what will happen when these two exogenous strains are co-transinfected into a new host. Here, we...

Summary NADH dehydrogenase (complex I) couples the oxidation of for reduction ubiquinone with generation a proton gradient that can be used synthesis ATP. We have found missense mutation in MT‐ND5 subunit Tibet chicken breed. In present study, mitochondrial respiratory control ratio (RCR) and activity embryonic brain different genotypes were measured. Significant differences between animals carrying mitochondria EF493865.1:m.1627A vs. EF493865.1:m.1627C alleles observed RCR enzyme activity.

Monocytes-macrophages play an indispensable role in the immune system. The current study investigated effect of selection for monocytes-macrophages phagocytosis on disease resistance generation 1 (G1) dwarf chickens. Five hundred chickens 0 (G0) were divided into high and low phagocytic index (PI) groups (HPIG LPIG, respectively) based their PI at 290 d age. Then, 2 x mating combinations conducted. Sixty G0 from another chicken group used to measure levels different developmental stages....

Abstract Wang, X.T., Zhao, C.J., Li, J.Y., Xu, G.Y., Lian, L.S., Wu, C.X. and Deng, X.M. 2010. Heme oxygenase-1 is important to the formation of eggshell biliverdin in chicken. J. Appl. Anim. Res., 38: 229–232. We postulated that pathway was similar bile attempted search some proofs. First, it found mean mRNA expression level heme (HO-1) shell glands Dongxiang blue-shelled hens (n-6) about 45.8 times more than Dwarf brown-shelled with method real time quantitative PCR. Second, solution hemin...

ABAQUS is used to create a detailed finite element model for 10-layer unbonded flexible riser simulate the riser’s mechanical behavior under three load conditions: tension force and internal external pressure. It presents technique analyze risers. In FEM model, all layers are modeled separately with contact interfaces; interaction between steel trips in certain has been considered as well. considering interaction, geometric nonlinearity, friction employed accurately structural of riser. The...

The problem of hypoxia adaptation in high altitudes is an unsolved brainteaser the field life sciences. As one best chicken breeds with adaptability to highland environment, Tibet chicken, genetically different from lowland breeds. It well known that has significant impact on growth by inducing expression alpha subunit heterodimeric transcription factor, hypoxia-inducible factor 1 (HIF1α). In present study, HIF1α brain, heart and liver tissues Dwarf Recessive White embryos were investigated...

Abstract Genome‐wide association studies have identified many single nucleotide polymorphisms (SNPs) associated with erythrocyte traits. However, the functional variants and their working mechanisms remain largely unknown. Here, we reported that SNP of rs80207740, which was red blood cell (RBC) volume hemoglobin content across populations, conferred enhancer activity to XPO7 gene via allele‐differentially binding Ikaros family zinc finger 1 (IKZF1). We showed region around rs80207740 an...

Crown gall in grapevine, caused by tumorigenic Agrobacterium vitis strains, can cause severe losses most viticulture regions worldwide. The only effective means of control is through cultivation practices. One non‐tumorigenic A. strain, E26, prevent crown infection when applied to wounds on grapevine prior or simultaneous with inoculation strains. ME19, a Tn5 mutant strain was impaired terms its ability be chemo‐attracted root tissue extracts and attachment roots, had reduced biological...