A5101827265- Lung Cancer Treatments and Mutations
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Cancer therapeutics and mechanisms
- HER2/EGFR in Cancer Research
- RNA Interference and Gene Delivery
- Transgenic Plants and Applications
- Insect Resistance and Genetics
- Mitochondrial Function and Pathology
- bioluminescence and chemiluminescence research
- Microbial Metabolites in Food Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Advanced biosensing and bioanalysis techniques
- Cytokine Signaling Pathways and Interactions
- Light effects on plants
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- Polyamine Metabolism and Applications
- Crystal Structures and Properties
- Hemiptera Insect Studies
- 14-3-3 protein interactions
- Pesticide Exposure and Toxicity
- Zeolite Catalysis and Synthesis
Guizhou University
2023
Henan University Huaihe Hospital and Huaihe Clinical Institute
2019-2023
State Key Laboratory of Medicinal Chemical Biology
2021-2022
Nankai University
2007-2022
Henan University
2021-2022
Affiliated Zhongshan Hospital of Dalian University
2018
South China Botanical Garden
2008-2009
Beijing Botanical Garden
2009
Chinese Academy of Sciences
2008
Peking University
1990-2005
Enzyme-regulated in situ self-assembly of peptides represents one versatile strategy the creation theranostic agents, which, however, is limited by strong dependence on enzyme overexpression. Herein, we reported self-amplifying assembly precisely macrophages associated with expression for improving anti-inflammatory efficacy conventional drugs. The assembling system was created via coassembling an enzyme-responsive peptide its derivative functionalized a protein ligand. Reduction NAD(P)H...
Mitochondrion-targeting therapy exhibits great potential in cancer but significantly suffers from limited therapeutic efficiency. Here we report on mitochondrion-targeting supramolecular antagonist-inducing tumor cell death via simultaneously promoting cellular apoptosis and preventing survival. The antagonist was created coassembly of a pentapeptide with its two derivatives functionalized BH3 domain or the drug camptothecin (CPT). While CPT released induced decreasing mitochondrial membrane...
A full-length cDNA of an acyl-acyl carrier protein (ACP) thioesterase (TE) (EC 3.1.2.14), named JcFATB1, was isolated from the woody oil plant Jatropha curcas L. The deduced amino acid sequence shares about 78% identity with FATB TEs, but only 33% FATA TEs other plants. also contains two essential residues (H317 and C352) for TE catalytic activity a putative chloroplast transit peptide at N-terminal. Southern blot analysis revealed that single copy JcFATB1 is present in J. genome,...
Abstract A small cysteine-rich protein with antimicrobial activity was isolated from pokeweed (Phytolacca americana) seeds and purified to homogeneity. The inhibits the growth of several filamentous fungi gram-positive bacteria. highly basic, a pI higher than 10. entire amino acid sequence determined be homologous (AMP) Mirabilis jalapa. cDNA encoding P. americana AMP (Pa-AMP-1) chromosomal DNA containing gene were cloned sequenced. deduced shows presence signal peptide at terminus,...
A cDNA clone encoding a putative β-ketoacyl-acyl carrier protein (ACP) synthase III (KAS III) was isolated from Jatropha curcas L., woody oil plant. The (named JcKAS contained 1203-bp open reading frame coding for 400 amino acids with predicted molecular mass of about 42 kDa. deduced acid sequence the shares 80% identity to KAS other plants, and contains conserved Cys176 in active site motif G355NTSAAS361 which is responsible binding regulatory acyl-ACPs. Southern blotting analysis indicated...
In an attempt to isolate cDNA clones for dark-inducible chloroplast proteins, we screened a library which was prepared from radish cotyledons by two-step method. The source plants were grown under continuous light 14 d and kept in darkness 24 h. One of the selected clones, S2D12, corresponded din1 gene previously reported as dark-inducible, senescence-associated [Azumi Watanabe (1991) Plant Physiol. 95: 577]. A 22 kDa polypeptide produced vitro expression system presence [35S]methionine....
A portion of the rbcS gene that encoded transit peptide and 20 amino acid residues N-tenninal region small subunit ribulosebisphosphate carb-oxylase/oxygenase was fused to 5' end psbA which encodes Dl protein PSII reaction center. The chimeric expressed in vitro as a 42-kDa protein, imported into chloroplasts isolated from pea leaves. processed such lost stroma, resultant translocated thylakoid membranes, C-ter-minal then removed yield mature with an N-terminal extension originated sub-unit....
Abstract Hemiptera insects exhibit a close relationship to plants and demonstrate diverse range of dietary preferences, encompassing phytophagy as the predominant feeding habit while minority engages in carnivorous or haematophagous behaviour. To counteract challenges posed by phytophagous insects, have developed an array toxic compounds, causing significant evolutionary selection pressure on these insects. In this study, we employed comparative genomics approach analyse expansion...
Abstract Background Tyrosine kinase inhibitors (TKIs) therapy is a standard treatment for patients with advanced non-small-cell lung carcinoma (NSCLC) when activating epidermal growth factor receptor ( EGFR ) mutations are detected. However, except the well-studied mutations, most lack regimens. Methods We constructed two variant libraries containing substitutions, deletions, or insertions using saturation mutagenesis method. All variants were located in mutation hotspot (exons 18–21). The...
Transfection efficiency was estimated to optimize the conditions for RNA interference (RNAi), including transfection time, validity, and nucleic acid concentration type, using EZ Trans Cell Reagent, a cationic polymer. An shRNA against GFP designed transfected into cells reagent. The significantly decreased expression of GFP. In addition, pre-diluted reagent at room temperature small acids increased efficiency, which peaked 24 h. Compared with circular acids, linear showed higher genome...
Background: Tyrosine kinase inhibitors is a standard treatment for patients with NSCLC harboring epidermal EGFR mutations.Methods: We conducted systematic cytotoxicity screening of mutations on five TKIs based deep mutational scanning. constructed two variant libraries containing substitutions, deletions, or insertions. All the variants were located in mutation hotspot.Findings: The sensitivity these to afatinib, erlotinib, gefitinib, icotinib, and osimertinib was systematically studied by...