A5087578815- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Urological Disorders and Treatments
- Urinary and Genital Oncology Studies
- Advanced Cellulose Research Studies
- Wound Healing and Treatments
- Urinary Bladder and Prostate Research
- Membrane Separation Technologies
- Natural Language Processing Techniques
- Bone Tissue Engineering Materials
- Ureteral procedures and complications
- Biomedical Text Mining and Ontologies
- Urologic and reproductive health conditions
- Connexins and lens biology
- Topic Modeling
- Surgical Sutures and Adhesives
- Nanopore and Nanochannel Transport Studies
- Silk-based biomaterials and applications
- Sexual function and dysfunction studies
- Pelvic and Acetabular Injuries
- Artificial Intelligence in Law
- Autophagy in Disease and Therapy
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Radiology practices and education
Shanghai Jiao Tong University
2015-2025
Renji Hospital
2017-2025
Shanghai Sixth People's Hospital
2013-2023
Binzhou Medical University
2021
Indiana University School of Medicine
2017
State Council of the People's Republic of China
2016
Zero to Three
2015
In this study, we built a bilayer nanofibrous material by utilizing the gelatinization properties of potato starch (PS) to interrupt bacterial cellulose (BC) assembly during static culture create more free spaces within fibrous network. Then, muscle cells were cultured on loose surface BC/PS scaffolds build biomaterials for hollow organ reconstruction. Our results showed that exhibited similar mechanical characters those in traditional BC scaffolds. And pore sizes and porosities could be...
Rationale:In urethral tissue engineering, the currently available reconstructive procedures are insufficient due to a lack of appropriate scaffolds that would support needs various cell types.To address this problem, we developed bilayer scaffold comprising microporous network silk fibroin (SF) and nanoporous bacterial cellulose (BC) evaluated its feasibility potential for long-segment regeneration in dog model.Methods: The freeze-drying self-assembling method was used fabricate by...
The goal of this study was to evaluate the effects urethral reconstruction with a three-dimensional (3D) porous bacterial cellulose (BC) scaffold seeded lingual keratinocytes in rabbit model. A novel 3D BC prepared by gelatin sponge interfering fermentation process. Rabbit were isolated, expanded, and onto BC. alone (group 1, N = 10), 2, 3, 10) used repair ventral defects (2.0 × 0.8 cm). Scanning electron microscopy revealed that consisted compact laminate while composed sheet buttressed...
The limited amount of available epithelial tissue is considered a main cause the high rate urethral reconstruction failures. aim this study was to investigate whether epithelial-differentiated rabbit adipose-derived stem cells (Epith-rASCs) could play role epithelium in vivo functionally and be potential substitute urothelium. Substitution urethroplasty performed repair an anterior defect male New Zealand rabbits using Epith-rASCs seeded bladder acellular matrix grafts (BAMGs) after...
A significant problem limiting the application of bacterial cellulose (BC) nanofibrous scaffolds for tissue regeneration is nanoscale pores that inhibit cell infiltration and vascularization in their three-dimensional (3D) structure.
Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n 6 for 2, 4, and 12 weeks), BAMG-HS weeks) cystotomy control groups. Equipped good cytocompatibility superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 0.69 N), acted trilayer "sandwich" scaffold minimal interference...
Cisplatin is a commonly used chemotherapy drug for prostate cancer (PC). However, some PCs are resistant to cisplatin treatment, while the molecular mechanisms underlying resistance of not completely understood. In this study, we found that dose-dependently activated Beclin-1 in two PC cell lines, PC3 and LNCap. Autophagy suppression significantly increased cisplatin-induced death these cells CCK-8 assay. Moreover, microRNA (miR)-144 levels were downregulated cisplatin-treated cells,...
Background: Cavernous nerve injury (CNI) is the leading cause of erectile dysfunction (ED) after radical prostatectomy and pelvic fracture. Transplantation human adipose-derived stem cells (ASCs) has been widely used to restore function in CNI-ED rats patients. Umbilical cord blood-derived MSCs (CBMSCs) are similarly low immunogenic but much primitive compared ASCs more promising large-scale commercial applications due extensive establishment blood banks. However, whether CBMSCs have...
To evaluate the effect of tissue inhibitor metalloproteinase-1 small interfering RNA (TIMP-1 siRNA) transfected fibroblasts (FB) for urethral reconstruction.A ventral mucosal defect was created. Substitution urethroplasty performed with intestinal submucosa (SIS) alone (8 rabbits, group 1), autogenic oral keratinocytes (OK)-seeded SIS 2) or OK and TIMP-1 siRNA FB-seeded 3). At 1 6 months after surgery (4 rabbits at each time point), retrograde urethrogram histologic analysis were to outcomes...
ABSTRACT Seeding cells efficiently and uniformly onto three‐dimensional scaffolds is key for engineering urological tissue with an ideal histological structure in vitro . Using optimized seeding technology allows to cooperate positively biomaterials, resulting successful reconstructive surgery. In this study, we used four different types of methods a scaffold small intestinal submucosa (SIS). The efficiency the sandwich co‐culture, layered static‐agitation seeding, centrifugation were...
// Dongdong Xiao 1, * , Qiong Wang 2, Hao Yan 1 Xiangguo Lv Yang Zhao Zhe Zhou Ming Zhang Qian Sun 3 Kang Wei Li 3, ** and Mujun Lu Department of Urology Andrology, Ren Ji Hospital, School Medicine, Shanghai Jiao Tong University, 200001, China 2 Urology, The Yat-sen Memorial Guangzhou 510120, State Key Lab Metal Matrix Composites, Materials Science Engineering, 200240, These authors contributed equally are co-first this manuscript co-corresponding Correspondence to: Lu, email:...
The sonourethrogram is a useful alternative to the traditional retrograde urethrogram evaluate anterior urethral strictures. With development of 3-dimensional reconstructive techniques imaging can provide more accurate and information enable surgeon make best surgical decisions. We evaluated accuracy efficacy reconstructed digital model urethra based on assess disease.A total 50 patients with an stricture 10 healthy volunteers were enrolled in this study from April 2014 January 2017. All...
Large language models (LLMs) are gaining increasing interests to improve clinical efficiency, owing their unprecedented performance in modelling natural language. Ensuring the reliable applications, evaluation of LLMs indeed becomes critical for better mitigating potential risks, e.g., hallucinations. However, current methods heavily rely on labor-intensive human participation achieve human-preferred judgements. To overcome this challenge, we propose an automatic paradigm tailored assess...