A5040780311- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Chromatography
- Pluripotent Stem Cells Research
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Toxic Organic Pollutants Impact
- Pancreatic function and diabetes
- Nanoparticle-Based Drug Delivery
- Electrospun Nanofibers in Biomedical Applications
- Bioactive Compounds and Antitumor Agents
- Characterization and Applications of Magnetic Nanoparticles
- Nanomaterials for catalytic reactions
- Neurological Disease Mechanisms and Treatments
- Biosensors and Analytical Detection
- Thyroid Disorders and Treatments
- Metabolism and Genetic Disorders
- Pelvic and Acetabular Injuries
- RNA and protein synthesis mechanisms
- Advanced Breast Cancer Therapies
- Dendrimers and Hyperbranched Polymers
- Cerebrovascular and genetic disorders
- Vitamin C and Antioxidants Research
- Chromatography in Natural Products
Shantou University
2022-2025
Hangzhou Medical College
2023-2025
Affiliated Hospital of Southwest Medical University
2024
Anhui Jianzhu University
2024
Zhejiang University of Technology
2023
Tianjin Huanhu Hospital
2021
Chengde Medical University
2021
Rice University
2016-2019
Zhejiang Academy of Medical Sciences
2010-2017
Nantong Maternity and Child Health Hospital
2017
This paper describes a simple, mild, and environmentally friendly approach to synthesize polystyrene/Ag (PS/Ag) nanocomposite spheres, which makes use of both reducing stabilizing functions polyvinylpyrrolidone (PVP) in aqueous media. In this approach, monodisperse polystyrene (PS) are used as templates for the synthesis core-shell sulfonated first. Then, [Ag(NH(3))(2)](+) ions adsorbed onto surface PS template spheres via electrostatic attraction between -SO(3)H groups (grafted on spheres)...
Importance Trials have not demonstrated superiority of alteplase or tenecteplase vs standard care in patients with mild stroke and raised safety concerns. Prourokinase is an alternative fibrinolytic that may a favorable profile, the benefit-risk profile prourokinase unknown. Objective To investigate efficacy ischemic within 4.5 hours symptom onset. Design, Setting, Participants This was multicenter, prospective, open-label, blinded–end point randomized clinical trial conducted from November...
Safety and reliability of transgene integration in human genome continue to pose challenges for stem cell-based gene therapy. Here, we report a baculovirus-transcription activator-like effector nuclease system AAVS1 locus-directed homologous recombination induced pluripotent cells (iPSCs). This viral system, when optimized U87 cells, provided targeted efficiency 95.21% incorporating Neo-eGFP cassette was able mediate DNA insert up 13.5 kb. In iPSCs, with persistent expression achieved...
Organophosphate flame retardants (OPFRs) are a class of substances that pose potential risks to human health and ecosystems due their large-scale production, wide range applications, ubiquitous presence in the environment. With for long-range atmospheric transport (LRAT), OPFR pollution high-altitude areas has become an increasing concern. Herein, general pretreatment method OPFRs across various sample matrices was established combined with gas chromatography-mass spectrometry (GC-MS),...
<title>Abstract</title> Background Mitochondrial transplantation is a promising cure for many diseases associated with mitochondrial defects or ageing; however, reliable method mitochondria transfer still in urgent need. Method In this study, we assembled fusogenic and magnet-responsive cells (FMRCs), which were enucleated stem loaded Fe<sub>3</sub>O<sub>4</sub> nanoparticles further incorporated vesicular stomatitis virus glycoprotein G (VSV-G). Fusion was carried out the presence of...
<title>Abstract</title> Background While numerous studies have identified a strong association between academic performance and mental health, the direction of causality remains unclear. This study aims to examine whether impacts health or vice versa. Methods A multistage sampling survey was conducted among 11,659 medical students from 50 universities across country. Two mediation models were constructed for analysis: (1) <italic>Academic stress → Academic Mental health</italic> (AAM), (2)...
To enable rapid and accurate point-of-care DNA detection, we have developed a single-step, amplification-free nucleic acid detection platform, substrate-mediated autocatalysis of CRISPR/Cas12a (DSAC). DSAC makes use the trans-cleavage activity Cas12a target template-activated substrate for dual signal amplifications. employs two distinct types: one that enhances amplification other negatively modulates fluorescent signals. The positive inducer utilizes nicked- or loop-based substrates to...
Abstract Objectives Detecting point mutations with high sensitivity and specificity can be technically very challenging, but it is crucial for early diagnosis effective drug treatment of cancers. To enable ultrasensitive ultraspecific detection single-base in simple economical ways, we have developed an artificial base mismatches-mediated PCR (ABM-PCR) approach. Methods ABM-PCR was applied to quantitative (qPCR) droplet digital (ddPCR) platforms. The impact mismatches on the thermodynamic...
The AAVS1 locus is viewed as a 'safe harbor' for transgene insertion into human genome. In the present study, we report new method targeting in human-induced pluripotent stem cells (hiPSCs).We have developed two baculoviral transduction systems: one to deliver zinc finger nuclease (ZFN) and DNA donor template site-specific gene another mediate Cre recombinase-mediated cassette exchange system replace inserted with transgene.Our ZFN provided targeted integration efficiency of Neo-EGFP 93.8%...
Abstract Integrative gene transfer using retroviruses to express reprogramming factors displays high efficiency in generating induced pluripotent stem cells (iPSCs), but the value of method is limited because concern over mutagenesis associated with random insertion transgenes. Site-specific integration into a preselected locus by engineered zinc-finger nuclease (ZFN) technology provides potential way overcome problem. Here, we report successful human fibroblasts state pluripotency...
Synthetic Notch (synNotch) receptors have enabled mammalian cells to sense extracellular ligands and respond by activating user-prescribed transcriptional programs. Based on the synNotch system, we describe a cell-based in vivo sensor for cancerous cell detection. We attempted engineer synNotch-programmed macrophages cancer via urinary analysis of human chorionic gonadotropin (HCGB5). Principally, when bind cells, is activated undergoes intramembrane proteolysis release activator into...
Enucleated cells, also known as cytoplasts, are valuable tools with a wide range of applications. However, their potential for bio-engineering is greatly restricted by the short lifespan. We postulated that enucleation process damages integrity plasma membrane and thus activates cell death program(s). The results showed tiny hole was generated transiently on when nucleus spun off, while force-gated ion channels were activated in response to pulling nucleus. Influx extracellular calcium...
Virus-derived gene transfer vectors have been successfully employed to express the transcription activator-like effector nucleases (TALENs) in mammalian cells. Since DNA-binding domains of TALENs consist variable di-residue (RVD)-containing tandem repeat modules and virus genome with repeated sequences is susceptible genetic recombination, we investigated several factors that might affect TALEN cleavage efficiency baculoviral vectors. Using a system designed target AAVS1 locus, observed...