A5059364419- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Crystal structures of chemical compounds
- Glioma Diagnosis and Treatment
- Synthesis and biological activity
- CRISPR and Genetic Engineering
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- FOXO transcription factor regulation
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Phagocytosis and Immune Regulation
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Mechanisms of cancer metastasis
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Metal complexes synthesis and properties
- Analytical Chemistry and Chromatography
- Nerve injury and regeneration
- Genetic Syndromes and Imprinting
- Immune cells in cancer
- Telomeres, Telomerase, and Senescence
- Phenothiazines and Benzothiazines Synthesis and Activities
Tianjin Medical University
2018-2025
University of Alabama at Birmingham
2023
Hubei Polytechnic University
2013-2023
Huangshi Central Hospital
2007-2011
Surface topography dictates important aspects of cell biological behaviors. In our study, hierarchical micro-nano (SLM-AHT) with micro-scale grooves and nano-scale pores was fabricated compared smooth (S) irregular (SLA) surfaces to investigate mechanism involved in cell-surface interactions. Integrin α2 had a higher expression level on SLM-AHT surface S SLA surfaces, the levels osteogenic markers icluding Runx2, Col1a1, Ocn were concomitantly upregulated surface. Moreover, formation mature...
<title>Abstract</title> <bold>・Background: </bold>Malignant glioblastoma exhibits cellular senescence characterized by changing tumor microenvironment. Solute carrier family 6 member (SLC6A6), a multichannel transmembrane protein, plays crucial role in regulating cell proliferation, apoptosis, differentiation and However, the molecular mechanism of SLC6A6 remains unknown. Our study aimed to elucidate regulatory mechanisms proliferation cells. <bold>・Methods: </bold>Expression was examined...
Diffuse intrinsic pontine gliomas (DIPGs) are lethal pediatric brain tumors that frequently harbor H3K27M mutations and lack effective treatments. Here, our epigenomic analyses uncovered an enrichment of SP/KLF transcription factors in open chromatin regions specifically H3K27M-mutated DIPG cells compared to normal neural progenitor cells. SP1 depletion or inhibition DNA binding with EC-8042, optimized mithramycin analog, significantly suppressed the proliferation invasiveness H3K27M-DIPG A...
Glioblastoma multiforme (GBM) is the most common and aggressive brain tumor refractory to existing therapies. The oncogene BMI-1, a member of Polycomb Repressive Complex 1 (PRC1) plays essential roles in various human cancers becomes an attractive therapeutic target. Here we showed that BMI-1 highly expressed GBM especially enriched glioblastoma stem cells (GSCs). Then comprehensively investigated anti-GBM effects PTC-209, novel specific inhibitor BMI-1. We found PTC-209 efficiently...
Abstract Follicular helper T (Tfh) cells are essential for germinal center (GC) B cell responses. However, it is not clear which PD-1 + CXCR5 Bcl6 CD4 will differentiate into hi GC-Tfh and how differentiation regulated. Here, we report that the sustained Tigit expression in marks precursor Tfh (pre-Tfh) to transition, whereas – upregulate IL-7Rα become memory with or without CCR7. We demonstrate pre-Tfh undergo substantial further at transcriptome chromatin accessibility levels cells. The...
Abstract Magnetic poly(acrylic acid‐acrylamide‐butyl methacrylate) (P(AAB)) nanocomposite hydrogels were prepared and used as adsorbents for removal separation of cationic dyes from aqueous solution. These magnetic P(AAB) characterized by scanning electron microscopy (SEM) vibrating sample magnetometer (VSM). It was found that these had responsive characters. The dynamic swelling, removal, dye, crystal violet (CV), basic magenta (BM) studied. adsorption capacity isotherm studies onto have...
Background: Dimethyl fumarate (DMF) has been approved for clinical treatment of multiple sclerosis based on its antioxidant and anti-inflammatory effects by activating the Nrf2 pathway. Since both oxidative stress inflammation are involved in Alzheimer’s disease (AD), DMF is a potential therapeutic option AD. Objective: This study aims to test AD model mice reveal underlying molecular mechanisms. Methods: Cell viability assay vitro immunofluorescence imaging were used evaluate effect...
Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated (Cas) 9 has been widely used far beyond genome editing. Fusions of deactivated Cas9 (dCas9) to transcription effectors enable interrogation the epigenome and controlling gene expression. However, large transgene size dCas9-fusion hinders its applications especially in somatic tissues. Here, we develop a robust CRISPR interference (CRISPRi) system by transgenic expression doxycycline (Dox) inducible dCas9-KRAB...
Background Glioblastoma (GBM) is the most common malignant brain tumor with poor clinical outcomes. Immunotherapy has recently been an attractive and promising treatment of extracranial malignancies, however, trials for GBM immunotherapy failed due to predominant accumulation tumor-associated microglia/macrophages (TAMs). Results High level LRIG2/soluble LRIG2 (sLRIG2) expression activates immune-related signaling pathways, which are associated prognosis in patients. LRIG2/sLRIGs promotes...
Abstract Mutations of epigenetic regulators are pervasive in human tumors. ASXL1 is frequently mutated myeloid malignancies. We previously found that forms together with BAP1 a complex can deubiquitinylate mono-ubiquitinylated lysine 119 on histone H2A (H2AK119ub1), Polycomb repressive mark. However, complete mechanistic understanding transcriptional regulation and tumor suppression remains to be defined. Here, we find depletion Asxl1 confers murine 32D cells IL3-independent growth at least...
Abstract CXCR5 is a hallmark of T follicular helper (Tfh) cells. The mechanism induction, however, still incompletely understood. In this study, we report that in mice with the absence transcription factor Bach2, Th17-inducing cytokines IL-6 and TGF-β together induced expression vitro. Mechanistically, IL-6/STAT3 drove Cxcr5 promoter activity via upstream site 1 regulatory element, whereas enhanced permissive histone modifications, STAT3 binding to element was higher Bach2. Subsequently,...
In the title mol-ecule, C(13)H(10)N(2)O, dihedral angle between benzoxazole ring system and benzene is 11.8 (1)°. crystal structure, mol-ecules are linked by inter-molecular N-H⋯N hydrogen bonds π⋯π inter-actions [centroid-centroid distance = 3.6560 (15) Å] to form a two-dimensional network.
In the title compound, C(16)H(13)N(3)S(2), dihedral angle between two benzothia-zole ring systems is 20.41 (2)°. crystal structure, inter-molecular N-H⋯N hydrogen bonds link mol-ecules into a chain along b axis. The packing further stabilized by C-H⋯π stacking inter-actions involving systems.
In the title compound, C(14)H(19)N(3)O(2)S, N atom of dimethyl-amino group and S are displaced by 0.078 (2) 0.084 Å, respectively, from naphthalene ring plane. The 2-amino-ethyl has a coiled conformation with an N-C-C-NH(2) torsion angle 53.6 (4)°. crystal structure, inter-molecular N-H⋯N weak C-H⋯O hydrogen bonds link mol-ecules into chains along [001].
ABSTRACT CRISPR-Cas9 has been widely used far beyond genome editing. Fusions of deactivated Cas9 (dCas9) to transcription effectors enable interrogation the epigenome and controlling gene expression. However large transgene size dCas9-fusion hinders its applications especially in somatic tissues. Here, we develop a robust CRISPR interference (CRISPRi) system by transgenic expression doxycycline (Dox) inducible dCas9-KRAB mouse embryonic stem cells (iKRAB ESC). After introduction specific...
In the title compound, [Pb(NO3)2(C16H15N5)], PbII ion is chelated by three N atoms of a bis(1H-benzimidazol-2-ylmethyl)amine (IDB) ligand and two O from each nitrate ligands, resulting in an asymmetric seven-coordinate configuration. The crystal structure stabilized intermolecular N—H⋯O C—H⋯O hydrogen bonds π–π interactions, with ring-centroid separations 3.578 (5) 3.686 Å, leading to three-dimensional network.
In the title mol-ecule, C(15)H(15)N(3)O(2)S, dihedral angle between naphthalene ring system and imidazole is 86.1 (2)°. crystal structure, weak inter-molecular C-H⋯O C-H⋯N hydrogen bonds, as well C-H⋯π inter-actions, connect mol-ecules, forming a two-dimensional network.
Abstract T follicular helper (Tfh) cells were discovered as CD4 +T in the B cell follicles that express CXCR5 and help germinal center (GC) responses. However, it is not clear which PD-1 +CXCR5 +Bcl6 +CD4 will differentiate into hiCXCR5 hiBcl6 hiGC-Tfh while others have a different fate, nor how GC-Tfh differentiation regulated. Here we report sustained Tigit expression marked precursor Tfh (pre-Tfh) to transition, whereas −PD-1 upregulated IL-7Ra become memory precursor/memory cells—with or...