A5031007311- Organ Transplantation Techniques and Outcomes
- Liver physiology and pathology
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Galectins and Cancer Biology
- Enzyme Production and Characterization
- Peptidase Inhibition and Analysis
- Cancer Treatment and Pharmacology
- Transplantation: Methods and Outcomes
- Blood Coagulation and Thrombosis Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Veterinary Orthopedics and Neurology
- Cardiovascular Effects of Exercise
- Erythropoietin and Anemia Treatment
- Intestinal Malrotation and Obstruction Disorders
- Phagocytosis and Immune Regulation
- Immunotherapy and Immune Responses
- Pituitary Gland Disorders and Treatments
Gifu University
2023-2024
Kumamoto University
1972-1997
Duke Medical Center
1989
Prefectural University of Kumamoto
1986
NewYork–Presbyterian Hospital
1971-1973
Cornell University
1971-1973
We investigated the role of hepatic macrophages in inflammatory response following reperfusion injury by blocking Kupffer cell phagocytosis with gadolinium chloride (GdCl3). Liver ischemia was induced rats occluding portal vein for 30 minutes. A bolus GdCl3 (7 mg/kg) injected intravenously 1 and 2 days before surgery. The serum levels cytokine–induced neutrophil chemoattractant (CINC) untreated increased reperfusion, peaked after 6 hours, then gradually decreased. or heparin alone...
We investigated the role of anticoagulant in ischemia/reperfusion injury liver, using activated protein C (APC), active human urinary thrombomodulin (UTM), and factor Xa blocked at site (DEGR-Xa). Liver ischemia was induced male Wistar rats by occlusion portal vein with a microvascular clip for 30 minutes. Each injected intravenously 10 minutes before clamping vein. Serum concentrations cytokine-induced neutrophil chemoattractant (CINC) were determined enzyme-linked immunosorbent assay. The...
Oxaliplatin, a platinum-based anticancer drug, is associated with peripheral neuropathy (oxaliplatin-induced neuropathy, OIPN), which can lead to worsening of quality life and treatment interruption. The endothelial glycocalyx, fragile carbohydrate-rich layer covering the luminal surface cells, acts as an gatekeeper has been suggested protect nerves, astrocytes, other cells from toxins substances released capillary vessels. Mechanisms underlying OIPN role glycocalyx remain unclear. This...
Galectin-3 is a beta-galactoside-binding lectin that plays important roles in diverse physiological functions, such as cell proliferation, apoptosis, and mRNA splicing. This protein expressed on inflammatory cells acts local mediator. Recently, galectin-3 has been detected several diseases, chronic liver, heart, kidney diabetes, viral infection, autoimmune neurodegenerative tumors, its role biomarker attracted attention. Alpha-galactosylceramide an artificially synthesized sphingolipid can...
The success rate of flap tissue reconstruction has increased in recent years owing to advancements microsurgical techniques. However, complications, such as necrosis, are still more prevalent diabetic patients compared non-diabetic individuals, presenting an ongoing challenge. To address this issue, many previous studies have examined vascular anastomoses dilation and stability, primarily concerning surgical techniques or drugs. In contrast, the present study, we focused on microvascular...
Colorectal cancer represents one of the most serious complications inflammatory bowel disease. The NLRP3 inflammasome plays a pivotal role in onset and progression disease is also implicated colorectal cancer. This study aimed to investigate whether deficiency or glyburide, sulfonylurea used for diabetes management known as an inhibitor, could suppress colitis its related tumorigenesis. Mice were divided into three groups: control group, glyburide NLRP3-deficient group. We investigated acute...
A single intravenous injection of freshly heparinized blood from a donor-specific transfusion (DST) seven days before transplantation significantly prolongs the subsequent survival hepatic allografts ACI(RT1a) to LEW(RT1(1)) rats. We used W3/25 (anti-CD4) and OX22 (anti-CD45RC: an isoform leukocyte-common antigen [CD45R]) monoclonal antibodies investigate cellular identity allograft infiltrates following transplantation. The number CD4+ CD45RC+ cells in untreated increased equally over time...
Acute pancreatitis is an aseptic inflammation caused by pathologically activated pancreatic enzymes and inflammatory mediators produced secondarily neutrophils other cells one of the most difficult diseases to treat. This study aimed investigate role in examining tissue dynamics.We created a model caerulein-induced 12-week-old male granulocyte colony-stimulating factor knockout mice (G-CSF-KO) wild-type littermate control (six intraperitoneal injections caerulein [80 μg/kg body weight] at...
The kinetics of messenger RNA (mRNA) and protein levels cytokine-induced neutrophil chemoattractant (CINC) in rat hepatic allografts during acute rejection were investigated. Infiltrating cells identified by double immunostaining with anti-CINC anti-macrophage monoclonal antibodies, ED1 ED2. serum CINC concentration untreated allograft recipients increased significantly at a constant rate over time after transplantation. No significant increases concentrations observed isografts or treated...
Gans, H. M.D., Ph.D.; Mori, K. M.D.; Quinlan, R. Richter, D. Tan, B. Ph.D. Author Information
Abstract Background Rhabdomyolysis is characterized by the destruction and necrosis of skeletal muscle tissue, resulting in acute kidney injury (AKI). Recombinant antithrombin (rAT) has DNA repair vascular endothelial-protection properties. Herein, we investigated whether rAT therapy beneficial effects against rhabdomyolysis-induced AKI. Ten-week-old male B6 mice were injected with 5 mL/kg 50% glycerol intramuscularly left thigh after 24 h fasting to create a rhabdomyolysis mouse model....
The mesothelium is a non-adhesive protective surface that lines the serosal cavities and organs within body. glycocalyx complex structure coats outer layer of mesothelium. However, due to limitations conventional fixation techniques, studies on glycans are limited. In this study, lectin staining frozen tissues was performed investigate diversity in mesothelial cells mice. Datura stramonium (DSL), which recognizes lactosamine binds Galectin-3 -1, broadly bound visceral parietal peritoneum but...
Vincristine-induced peripheral neuropathy (VIPN) adversely affects the quality of life and treatment continuity patients. The endothelial glycocalyx (eGCX) protects nerves from harmful substances released capillary vessels, but its role in remains unclear. We investigated impact eGCX protection on VIPN. Using a murine model VIPN, we administered nafamostat mesylate to protect shedding, analyzed integrity manifestation neuropathy. Nafamostat suppressed allodynia associated with Additionally,...
In a previous study we have found that the plasma pancreatic polypeptide (PP) response to oral glucose loading is exaggerated in diabetic patients compared with normal subjects. We investigated, therefore, effects of protein-rich meal or meat extract ingestion on PP secretion and examined also intravenous arginine administration levels subjects noninsulin-dependent diabetes mellitus (NIDDM). Following 600 Kcal ingestion, increased immediately showed biphasic NIDDM, but was NIDDM. A 50 g...
The ability of rat serum to inactivate endotoxin (LPS) was assessed with the aid limulus amebocyte lysate assay. Following addition various amounts normal mixture incubated for 1 hr at 37°C and residual activity determined. One milliliter inactivated between 5 10 μg Escherichia coli LPS per hour. Heating 45 min 56 °C resulted in loss 80–90% inhibitor (LPSI) activity. Serum from cobra venom factor (CVF)-treated rats 0.5 2.5 LPS/ml serum. tolerant rats, even after heating 56°C, inactivates 15...