Rhino-orbito-cerebral mucormycosis(ROCM) is an invasive fungal infection that usually occurs in immunocompromised patients and sometimes presents as orbital apex syndrome(OAS) initially. It rapidly fatal without early diagnosis treatment. We report the cases of ROCM presenting with OAS initially order to raise attention clinicians. retrospectively investigated eleven admitted between January 2006 December 2013. analyzed clinical features, results laboratory radiological examinations, nasal...

Purpose: The purpose of this study was to investigate the therapeutic effect perillaldehyde (PAE) on Aspergillus fumigatus (A. fumigatus) keratitis. Methods: Human corneal epithelial cells (HCECs) were pretreated with PAE and stimulated A. mycelium. C57BL/6 mice infected treated or without 1 day after infection. Clinical scores, PCR, ELISA, Western blotting used detect expression pro-inflammatory mediators, dendritic cell-associated c-type lectin-1 (Dectin-1), nuclear factor...

Purpose.: To determine the role of lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1) in corneal epithelial cells exposed to fungus. Methods.: Thirteen corneas with fungal keratitis composed experimental group. Ten healthy donor were control Corneal epithelium was scraped and LOX-1 mRNA tested. Immunostaining used detect protein. Human (HCECs) treated 75% ethanol-killed Aspergillus fumigatus. detected, CXCL1, TNF-α, IL-6, dectin-1 levels detected or without neutralization LOX-1....

To evaluate the visual quality of patients with different angle κ sizes after a trifocal diffractive intraocular lens (IOL) implantation.The Affiliated Hospital Qingdao University, Qingdao, China.Prospective case series.Patients who had phacoemulsification implantation IOL AT LISA tri 839MP were enrolled in study. The divided into 3 groups based on size preoperative κ. Monocular far, intermediate, and near uncorrected acuities measured during 3-month follow-up. Other outcome measurements...

Retinal neovascularization (RNV) is a typical feature of ischemic retinal diseases that can lead to traction detachment and even blindness in patients, which the vascular endothelial cell growth factor (VEGF) plays pivotal role. However, most anti-VEGF drugs currently used for treating RNV, such as ranibizumab, need frequent repeated intravitreal injections due their short half-life, increases incidence complications. Herein, hydrogel drug delivery system (DDS) prepared by dynamic Schiff...

PURPOSE: To compare visual function after phacoemulsification with implantation of a multifocal intraocular lens (IOL) or monofocal IOL. SETTING: Department Ophthalmology, Affiliated Hospital Qingdao University Medical College, Qingdao, Shandong, China. METHODS: This study comprised patients unilateral cataract who had an AcrySof ReSTOR SA60D3 IOL (multifocal group) SA60AT single-piece (monofocal group). Postoperative function, including uncorrected (UDVA) and corrected (CDVA) distance...

Purpose: To explore the influence of indoleamine 2,3-dioxygenase (IDO) on macrophage recruitment, polarization and phagocytosis in Aspergillus fumigatus keratitis. Methods: A murine model A. keratitis peritoneal macrophages incubated with hyphae were used. Macrophage recruitment corneas was evaluated using immunofluorescence staining. The macrophages, which stimulated by pretreatment or without 1-methyltryptophan (1-MT), interferon gamma (IFNG), extracellular regulated protein kinases (ERK)...

To construct and validate a prediction model for enteral feeding intolerance in critically ill patients during the first 7 days of feeding. A retrospective cohort study. We reviewed medical records two intensive care units from January 2015 to August 2023, develop by univariate analysis logistic regression analysis. Model's performance was evaluated through discrimination, calibration decision curve This study involved total 471 patients, with an incidence rate 35.7%. The comprised six...

Indoleamine 2,3-dioxygenase (IDO), which is mainly expressed in activated dendritic cells, known as a regulator of immune responses. However, the role IDO responses against fungal corneal infection has not been investigated. To evaluate regulatory mechanisms inflammation, we resorted to human epithelial cells (HCECs), first barrier cornea pathogenic microorganisms. We found that was significantly up-regulated epithelium infected with Aspergillus fumigatus (A. fumigatus) and HCECs incubated...

To observe Dectin-1 expression in fungal keratitis on rat models and to determine the role of innate immune response Aspergillus fumigatus. Wistar rats were randomly divided into control, pretreatment (pretreated with Laminarin) groups. Samples used for conducting immunohistochemical staining real-time PCR cytokines like CCL2, CCL3, CXCL1, CXCL2, IL-1β, TNF-α, IL-6, IL-10. After stimulations, all 7 inflammatory factors, except IL-10, increased different levels. 4 h CXCL1 CXCL2 groups...

To evaluate the effect of corneal graft diameter on therapeutic penetrating keratoplasty (PKP) for fungal keratitis.A total 116 patients (116 eyes) suffered from keratitis underwent PKP at Affiliated Hospital Medical College Qingdao University May 2006 to 2010. They were divided into two groups according diameter. 64 eyes' was 8.00mm or larger and 52 smaller than 8.00mm. The follow-up time 2 years. postoperative visual acuity complications documented compared.Sixty-two (96.88%) eyes fifty...

To explore the immunomodulatory effects of curdlan on innate immune responses against Aspergillus fumigatus (A. fumigatus) in cultured human corneal epithelial cells (HCECs), and whether C-type lectin receptor Dectin-1 mediates curdlan.The HCECs were stimulated by different concentrations (50, 100, 200, 400 µg/mL) for various time. Then pretreated with or without laminarin (Dectin-1 blocker, 0.3 mg/mL) A. hyphae. The mRNA protein production tumor necrosis factor-α (TNF-α) interleukin-6...

To explore the effects of lectin-like ox-LDL receptor (LOX-1) on innate immunity against Aspergillus fumigatus (A. ) in mice cornea.The mRNA levels LOX-1 were tested normal and A. infected corneas C57BL/6 BALB/c mice. The expression LOX-1, pro-inflammatory cytokines TNF-α, CXCL1 IL-6, anti-inflammatory IL-10, matrix metalloproteinase 9 (MMP9) with treatment neutralizing antibody or control IgG C57BL/6. Macrophages neutrophils extracted from susceptible mice, pretreated IgG, then stimulated...

Federated Learning (FL) is an emerging distributed machine learning (ML) technique that enables in-situ model training and inference on decentralized edge devices. We propose Totoro, a novel scalable FL engine, massive applications to run simultaneously networks. The key insight explore hash table (DHT)-based peer-to-peer (P2P) re-architect the centralized system design into fully one. In contrast previous studies where many shared one parameter server, Totoro assigns dedicated server each...

This study aims to explore the efficacy and safety of macrophage membrane-coated nanoparticles for delivery natamycin (NAT) in therapy fungal keratitis (FK). Macrophage membranes were isolated identified by immunofluorescence staining (IFS). NAT was encapsulated into poly(lactic-co-glycolic acid) (PLGA). Fungal stimulated (M1) or unstimulated (M) separately mixed sonicated with PLGA nanoparticles. The biocompatible (PLGA-NAT, PLGA-NAT@M, PLGA-NAT@M1) characterized zeta-sizer analysis,...

This study aimed to detect the early expression of macrophage-inducible C-type lectin (Mincle) and investigate its role in innate immune response fungal keratitis (FK).Wistar rats were used make models. The inflammatory responses corneal lesions observed by slit-lamp microscope.RT-PCR, immunohistochemistry, immunofluorescence Mincle rat epithelium. eight cytokines (CXCL1, CXCL2, IL-1β, IL-6, IL-10, TNF-α, CCL2, CCL3) detected real-time RT-PCR immunohistochemistry. Lastly, epithelium 54...