A5110365082- Genetic and Kidney Cyst Diseases
- Hedgehog Signaling Pathway Studies
- Renal and related cancers
- Biomedical Research and Pathophysiology
- Immune Response and Inflammation
- Alkaloids: synthesis and pharmacology
- Berberine and alkaloids research
- Biochemical effects in animals
- Influenza Virus Research Studies
- Cell death mechanisms and regulation
- Lipid metabolism and disorders
- RNA Interference and Gene Delivery
- Stress Responses and Cortisol
- Neuroinflammation and Neurodegeneration Mechanisms
- interferon and immune responses
- Genetic Syndromes and Imprinting
- Cancer-related molecular mechanisms research
- Regulation of Appetite and Obesity
- Calcium signaling and nucleotide metabolism
- Extracellular vesicles in disease
- Mass Spectrometry Techniques and Applications
- Alcohol Consumption and Health Effects
- Thermal Regulation in Medicine
- Hydrogen's biological and therapeutic effects
- Bone Metabolism and Diseases
Jinan University
2009-2025
Ningbo No. 2 Hospital
2021-2023
Third Affiliated Hospital of Sun Yat-sen University
2023
Sun Yat-sen University
2023
State Administration of Traditional Chinese Medicine of the People's Republic of China
2011-2018
University of Hong Kong
2012
ABSTRACT Metastasis is a major cause of poor prognosis pancreatic cancer. Exosomes (Exos) regulate cancer progression by modulating macrophage polarization. This study aimed to investigate the effects cancer‐associated fibroblast (CAF)‐released Exos on polarization in and molecular mechanisms. THP‐1 cells or xenografted tumor mice were treated with from CAFs, was analyzed using quantitative real‐time PCR (qPCR) flow cytometry. cocultured BXPC‐3 cells, metastasis Transwell assay scratch test....
Myocardial dysfunction is a common complication in sepsis and significantly contributes to the mortality of patients with septic shock. Our previous study demonstrated that pretreatment berberine (Ber) protected against lethality induced by LPS, which was enhanced yohimbine, an [alpha]2-adrenergic receptor antagonist, Ber combined yohimbine also improved survival mice subjected cecal ligation puncture. However, no studies have examined whether reduce LPS-induced myocardial dysfunction. Here,...
Ovarian cancer is a main contributor of cancer-relevant deaths among women worldwide due to high incidence and mortality. Mounting evidence has unveiled that lncRNAs play critical roles in malignancies, including ovarian cancer. Although the tumor suppressor function HCG11 prostate glioma been proved, investigations on role are still scarce.Gene or protein expression was quantified by RT-qPCR western blot. effects were assessed functional assays. Bioinformatics analysis mechanism experiments...
Cardiomyopathy is a common complication associated with increased mortality in sepsis, but lacks specific therapy. Here, using genetic and pharmacological approaches, we explored the therapeutic effect of α2A-adrenergic receptor (AR) blockade on septic cardiomyopathy. CLP-induced rats were treated BRL44408 (α2A-AR antagonist), prazosin (α1-AR antagonist) and/or reserpine. cardiomyopathy, indicated by reduced dP/dt cardiac troponin I phosphorylation, was attenuated BRL44408, this TNF-α...
Abstract This study was designed to determine whether the use of acetylcholinesterase inhibitors (AChEIs), a group drugs that stimulate acetylcholine receptors and are used treat Alzheimer's disease (AD), is associated with osteoporosis protection inhibition osteoclast differentiation function. Firstly, we examined effects AChEIs on RANKL‐induced function osteoclastogenesis bone resorption assays. Next, investigated impacts nuclear factor κB NFATc1 activation expression marker proteins CA‐2,...
The ongoing pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome 2 (SARS-CoV-2) poses a serious threat to global public health and there is currently no effective antiviral therapy. It has been suggested that chloroquine (CQ) hydroxychloroquine (HCQ), which were primarily employed as prophylaxis treatment for malaria, could be used treat COVID-19. CQ HCQ may potential inhibitors SARS-CoV-2 entry into host cells, are mediated via...
Background Previous studies have reported that cancer stem cells (CSCs) play a key role in tumorigenesis, metastasis, and recurrence. CSC‐based vaccination confers better protection tumor cells. However, isolation cultivation of CSCs are difficult. This study aimed to explore the similarities between induced pluripotent (iPSCs). Methods ALDH1+ were isolated from lung adenocarcinoma patients their gene expression patterns compared with human (hiPSCs). In addition, vaccine was developed using...
Abstract The aim of the present study was to investigate molecular mechanism miR‐182 in kidney fibrosis polycystic disease (PKD). We measured expression tissue autosomal dominant PKD. Additionally, we investigated relationship between and fibrotic protein by transfecting mimics inhibitor into cyst‐lined epithelial cells, respectively. Furthermore, observed interaction transforming growth factor β1 (TGF‐β1) fibrinogen factors cells after TGF‐β1 intervention, Smad2 Smad3 protein. Results are...
<b><i>Background:</i></b> Histone deacetylase inhibitors (HDACi) have therapeutic effects on various models of renal diseases including autosomal dominant polycystic kidney disease (ADPKD), but the molecular mechanism is unclear. <b><i>Objectives:</i></b> Here, we studied role trichostatin A (TSA), a specific HDACi, in regulating cyst growth to test possibility that HDACi might help manage ADPKD by enhancing autophagy....
This study was designated to establish a polycystic ovary syndrome (PCOS) rat model with recombinant human insulin-like growth factor-1 (RH-IGF-1). We made assessment on the characteristics of hyperinsulinemia and hyperandrogenism in model.This performed PCOS upon RH-IGF-1 injection evaluated disease process caused by insulin-resistant pathological condition IGF-1 based comparative vivo test.The experiment conducted experimental research center Yinzhou NO.2 hospital, Ningbo, Zhejiang...
Abstract Transcriptional regulation plays an essential role in the self-renewal and differentiation of human embryonic stem cells (hESCs). However, how external signals disrupt regulatory network further drive hESC remains largely unknown. Here, we found immune regulative protein, gamma-interferon-inducible protein 16 (IFI16) was involved both gene expression during trilineage specification through interaction with p53. IFI16 levels were upregulated JNK activation. knockdown delayed...
Abstract This study aims to investigate the effects of TNF receptors associated factor 3 (TRAF3) on signaling pathway and expression downstream products nuclear kappa B (NF‐κB) in epithelial cells renal ducts individuals with polycystic kidney disease (PKD). We observe TRAF3 genic overexpression cells, which form a tubular branch structure, kidneys explore protective effect cystogenesis progression PKD. Western blotting analysis was conducted examine changes NF‐κB PKD transgenic cells....
PYNOD, a nod-like receptors (NLR)-like protein, was indicated to inhibit NF-κB activation, caspase-1-mediated interleukin (IL)-1β release and cell apoptosis in dose-dependent manner. Exogenous addition of recombinant PYNOD mixed glial cultures may suppress caspase-1 activation IL-1β secretion induced by Aβ. However, the best our knowledge, there no study has focused on immunoregulatory effects specifically microglia. The present aimed explore roles involved lipopolysaccharides (LPS)-induced...
Specific types of nephroblastoma (Wilms' tumor, WT) are known to associate with poor overall survival. Emerging experimental evidence has demonstrated that competitive endogenous RNA (ceRNA) networks have important roles in regulating cancer occurrence, but the ceRNAs WT progression and patient outcomes remain unclear. Using multi-omics data 132 patients collected from TARGET database, an integration analysis pipeline was performed construct a highly reliable ceRNA network. As results, total...