A5008314778- Sepsis Diagnosis and Treatment
- Intensive Care Unit Cognitive Disorders
- Cardiac Ischemia and Reperfusion
- Berberine and alkaloids research
- Cardiac Arrest and Resuscitation
- Respiratory Support and Mechanisms
- Alkaloids: synthesis and pharmacology
- Synthesis and Biological Activity
- Anesthesia and Neurotoxicity Research
- Hemodynamic Monitoring and Therapy
- Cell death mechanisms and regulation
- Eicosanoids and Hypertension Pharmacology
- Receptor Mechanisms and Signaling
- Liver Disease Diagnosis and Treatment
- Plant-derived Lignans Synthesis and Bioactivity
- EEG and Brain-Computer Interfaces
- Antibiotic Resistance in Bacteria
- Inflammasome and immune disorders
- Phytochemistry and Biological Activities
- Simulation-Based Education in Healthcare
- Heart Rate Variability and Autonomic Control
- Phytochemical Studies and Bioactivities
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiac Fibrosis and Remodeling
- Genital Health and Disease
State Administration of Traditional Chinese Medicine of the People's Republic of China
2012-2024
Jinan University
2012-2024
Jilin University
2017
University of Electronic Science and Technology of China
2014
Cardiomyocyte apoptosis is an important event in doxorubicin (DOX)-induced cardiac injury. The aim of the present study was to investigate protection berberine (Ber) against DOX- triggered cardiomyocyte neonatal rat cardiomyocytes and rats. In cardiomyocytes, Ber attenuated DOX-induced cellular injury a dose-dependent manner. However, has no significant effect on viability MCF-7 breast cancer cells treated with DOX. reduced caspase-3 caspase-9, but not caspase-8 activity DOX-treated...
Doxorubicin (DOX)-related cardiotoxicity has been recognized as a serious complication of cancer chemotherapy. Effective targeted strategies for myocardial protection in addition to DOX treatment are urgently needed. The purpose this paper was determine the therapeutic effect berberine (Ber) on DOX-triggered cardiomyopathy and explore underlying mechanism. Our data showed that Ber markedly prevented cardiac diastolic dysfunction fibrosis, reduced malondialdehyde (MDA) level increased...
Caspase activation and cardiomyocyte apoptosis have been implicated in lipopolysaccharide (LPS)-induced cardiac contractile dysfunction. We recently demonstrated that β1-adrenoceptor (AR) by endogenous norepinephrine contributes to endotoxemic mice. Here, we further investigated the molecular mechanisms for enhancing effect of β₁-AR on LPS-induced apoptosis.The adult mouse ventricular myocytes were exposed LPS, dobutamine, protein kinase A (PKA) inhibitor or/and nifedipine, an L-type Ca(2+)...
Abstract Cardiomyocyte tumour necrosis factor α ( TNF ‐α) production contributes to myocardial depression during sepsis. This study was designed observe the effect of norepinephrine NE ) on lipopolysaccharide LPS )‐induced cardiomyocyte ‐α expression and further investigate underlying mechanisms in neonatal rat cardiomyocytes endotoxaemic mice. In cultured cardiomyocytes, inhibited ‐induced a dose‐dependent manner. 1 ‐ adrenoceptor AR antagonist (prazosin), but neither β nor 2 antagonist,...
Myocardial depression is an important contributor to mortality in sepsis. We have recently demonstrated that α2-adrenoceptor (AR) antagonist, yohimbine (YHB), attenuates lipopolysaccharide (LPS)-induced myocardial depression. However, the mechanisms for this action of YHB are unclear. Here, we decreased nitric oxide (NO) and tumor necrosis factor-alpha (TNF-α) levels myocardium plasma, attenuated cardiac hepatic dysfunction, but not kidney lung injuries endotoxemic mice. Immunohistochemical...
This study aims to examine the cross-sectional association between serum total bilirubin (STB) and type 2 diabetes (T2D) risk in general population, whether obesity could moderate this association.We used data from 1999-2018 National Health Nutrition Examination Surveys (NHANES), including a of 38,641 US adult participants who were 18 years or older. The STB was classified as low, moderate, high groups according tertiles.We found that with lower had significantly higher T2D than those (OR =...
Abstract Intrinsic cardiac adrenergic (ICA) cells regulate both developing and adult physiological pathological processes. However, the role of ICA in septic cardiomyopathy is unknown. Here we show that norepinephrine (NE) secretion from increased through activation Toll-like receptor 4 (TLR4) to aggravate myocardial TNF-α production dysfunction by lipopolysaccharide (LPS). In cells, LPS activated TLR4-MyD88/TRIF-AP-1 signaling promoted NE biosynthesis expression tyrosine hydroxylase, but...
The active center of selenium-containing glutathione peroxidase (GPx) is selenocysteine (Sec), which biosynthesized on its tRNA in organisms. decoding Sec depends a specific elongation factor and Insertion Sequence (SECIS) to suppress the UGA codon. expression mammalian GPx extremely difficult with traditional recombinant DNA technology. Recently, chimeric (tRNAUTu) that compatible Tu (EF-Tu) has made selenoprotein easier. In this study, human (hGPx) was expressed amber-less Escherichia coli...
Cardiomyopathy is particularly common in septic patients. Our previous studies have shown that activation of the alpha 1 adrenergic receptor (α
Cardiomyopathy is a common complication associated with increased mortality in sepsis, but lacks specific therapy. Here, using genetic and pharmacological approaches, we explored the therapeutic effect of α2A-adrenergic receptor (AR) blockade on septic cardiomyopathy. CLP-induced rats were treated BRL44408 (α2A-AR antagonist), prazosin (α1-AR antagonist) and/or reserpine. cardiomyopathy, indicated by reduced dP/dt cardiac troponin I phosphorylation, was attenuated BRL44408, this TNF-α...
Introduction Due to policy changes in the context of COVID-19 pandemic, online teaching has become main form class many Chinese universities. Flipped classroom been widely used other disciplines, but there is a dearth evidence available about use emergency medicine. This study aimed develop flipped for medicine and evaluate its effectiveness by comparing it with traditional lecture-based teaching.
The present study was aimed to observe the effect of berberine (Ber) on epirubicin (EPI)-induced growth inhibition, apoptosis, and cell cycle arrest in T24 bladder cancer cells.The cells were exposed EPI, with or without different concentrations Ber. viability measured by counting Kit-8, apoptosis determined Hoechst 33258 staining expression cleaved caspase-3, caspase-9, Bcl-2, Bax, P53 proteins detected Western blot assay. In addition, production reactive oxygen species (ROS) also...
Cardiomyopathy is a common complication and significantly increases the risk of death in septic patients. Our previous study demonstrated that post-treatment with dexmedetomidine (DEX) aggravates cardiomyopathy. However, mechanisms for side effect DEX on cardiomyopathy are not well-defined. Here we employed cecal ligation puncture (CLP) model α2A-adrenoceptor deficient (Adra2a-/-) mice to observe effects myocardial metabolic disturbances sepsis. CLP displayed significant cardiac dysfunction,...
The ongoing pandemic of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome 2 (SARS-CoV-2) poses a serious threat to global public health and there is currently no effective antiviral therapy. It has been suggested that chloroquine (CQ) hydroxychloroquine (HCQ), which were primarily employed as prophylaxis treatment for malaria, could be used treat COVID-19. CQ HCQ may potential inhibitors SARS-CoV-2 entry into host cells, are mediated via...
Dobutamine (DOB) is recommended as an inotrope for septic patients with low cardiac output, but its long-term impact on sepsis-induced cardiomyopathy remains unclear. This study investigated the effect of DOB myocardial dysfunction and injury. Rats were exposed to cecal ligation puncture (CLP), intrinsic function, other organ functions, hemodynamics, inflammatory response, serum injury biomarkers, apoptosis, vascular permeability determined. At 6 h after CLP, left ventricular ±dP/dt...
Dexmedetomidine (DEX), a selective α2 adrenergic receptor (AR) agonist, is commonly used as sedative drug during critical illness. In the present study, we explored novel accelerative effect of DEX on cardiac fibroblast (CF) differentiation mediated by LPS and clarified its potential mechanism. apparently increased expression α-SMA collagen I/III phosphorylation p38 Smad-3 in CFs mice. These effects were significantly enhanced through increasing α2A-AR after stimulation. The from knockout...
This study investigated the subtype distribution of human papillomavirus (HPV) in patients with condyloma accuminatum (CA) Shenzhen city, China, and assessed relationship between different HPV subtypes cervical neoplasia. Type-specific prevalence extent multiple infections were genital tract. CA samples collected from 352 departments dermatology gynecology People's Hospital during 2004-2006, using MY09/11 PCR reverse dot blot hybridization for genotyping 9-20 kinds subtypes. status was...