Abstract The high morbidity and mortality of colorectal cancer (CRC) is a major challenge in clinical practice. Although series alternative research models CRC have been developed, appropriate orthotopic animal that reproduce the specific response as well pathophysiological immune features are still lacking. In current study, we constructed xenograft model by implanting tumor tubes at colorectum mice monitored development using bioluminescence imaging. This successfully recapitulates...

Urban zones across the world have experienced a massive expansion in recent decades, affecting food production, carbon storage, and biodiversity, further threatening realization of UN's Sustainable Development Goals (SDGs), particularly SDG 2 (zero hunger), 13 (climate action), 15 (life on land). These enormous impacts are even more worrying coastal areas where urbanization has been continuously accelerated. However, we poor understanding about magnitude reduction natural habitat crucial...

Ecosystem services (ES) are used as a tool for eco-environment management, and this study establishes framework that integrates ecological red line (ERL), public perceptions of ES trade-offs among into regional management. The assessed using questionnaires statistical analyses. Three models (InVEST, i-Tree Eco, ROS), food supply calculation method GIS to quantify eight indicators (water yield, soil retention, water purification, biodiversity conservation, atmospheric regulation, outdoor...

Antibodies are an important reagent to determine the specificity and accuracy of diagnostic immunoassays for various diseases. However, traditional antibodies have several shortcomings due their limited abundance, difficulty in permanent storage, required use a secondary antibody. Nanobodies, which derived from single-chain camelid antibodies, can circumvent many these limitations and, thus, appear be promising substitute. In presented study, sandwich ELISA-like immunoassay direct...

Hepatitis E virus (HEV) is an emerging zoonotic pathogen that transmitted primarily through the fecal-oral route and can cause acute hepatitis in humans. Since HEV was identified as a pathogen, different species of strains have been globally from various hosts, leading to expanding range hosts. The genome consists 5' noncoding region, three open reading frames (ORFs), 3' region. ORF3 protein smallest but has many functions release pathogenesis. In this review, we systematically summarize...

Abstract Background Traditional sandwich enzyme-linked immunosorbent assay (ELISA) using polyclonal and monoclonal antibodies as reagents presents several drawbacks, including limited amounts, difficulty in permanent storage, required use of a secondary antibody. Nanobodies can be easily expressed with different systems fused tags their tertiary structure by recombinant technology, thus offering an effective detection method for diagnostic purposes. Recently, the fenobody (ferritin-fused...

Persistent human papillomavirus (HPV) infection is associated with multiple malignancies. Developing therapeutic vaccines to eliminate HPV-infected and malignant cells holds significant value. In this study, we introduced a lipid nanoparticle encapsulated mRNA vaccine expressing tHA-mE7-mE6. Mutations were into E6 E7 of HPV their tumourigenicity. A truncated influenza haemagglutinin protein (tHA), which binds the CD209 receptor on surface dendritic (DCs), was fused mE7-mE6 in order allow...

In 2020, severe diarrhea occurred in four-month-old fattening pigs from nine farms Shandong Province, China. Fecal samples were collected diseased and tested by PCR for the presence of mammalian orthoreovirus (MRV), porcine epidemic virus (PEDV), deltacoronavirus (PDCoV), rotavirus A (PoRVA), transmissible gastroenteritis (TGEV), kobuvirus (PKV), pseudorabies (PRV). The viral RNA MRV PEDV was detected fecal samples. genome sequences successfully amplified same sample. Genomic phylogenetic...

FOLFOX is the recommended chemotherapy regimen for colorectal cancer (CRC), but its response rate remains low. Our previous studies have established a close relationship between gut microbiota and anti-CRC effect of FOLFOX, though underlying mechanisms remain unclear. Diet has been confirmed as key factor influencing microbiota, high-salt diets, representative western dietary habits, shown to affect immune function, risk developing CRC. However, impact diets on efficacy unstudied. Therefore,...

Objectives Gastric motor dysfunction is a common symptom in patients with stroke, yet lacks objective evaluation methods. This study aimed to assess the feasibility of using gastric filling ultrasonography evaluate function undergoing and explore relationship between ultrasound indicators clinical features. Methods We conducted case-control where all participants underwent 60-minute examination after consuming 300 mL test meal. The cross-sectional area (CSA) antrum was measured at four-time...

Abstract Polyploidy occurs frequently in plants and is an important force plant evolution crop breeding. New polyploids face various challenges due to genome duplication subsequent changes epigenetic modifications, nucleus/cell size gene expression. How produce evolutionary novelty remains be understood. In this study, a transcriptome comparison between 21-day-old diploid autotetraploid pak choi seedlings revealed that there are few differentially expressed genes (DEGs), with greater...

Abstract Background Postoperative delirium (POD) is a frequent complication after surgery and its occurrence associated with poor outcomes. The neuropathology of this unclear, but it important to evaluate relevant biomarkers for postoperative status. purpose study explore the relationship between expression levels cholinergic in cerebrospinal fluid (CSF) development POD elderly patients. Methods Four hundred ninety-two patients aged 65 years old or older elective total hip/knee replacement...

Previous studies have demonstrated that tannin could inhibit the proliferation and angiogenesis of cancer cells. However, mechanism(s) associated with its antitumor effect remains unclear. Here, we investigated effects 3,3',4'-trimethylellagic acid (TMEA), a compound isolated from Sanguisorba officinalis L., on proliferation, angiogenesis, apoptosis in cells, as well underlying related to activity. TMEA was L. by silica gel column chromatography. Molecular docking carried out assess active...

Diabetic cardiomyopathy (DCM) is a main cause of heart failure and death in diabetic patients. However, countermeasures to limit the development this disease remain insufficient. Si-Miao-Yong-An decoction (SMYA), Chinese herbal prescription, exhibits both lipid-lowering cardiovascular preserving effects, may have an effect on DCM management. The current study aimed investigate effects SMYA cardiac function mice underlying mechanisms involved. Streptozotocin-induced were fed intragastrically...

HIGD1B (HIG1 Hypoxia Inducible Domain Family Member 1B) is a protein-coding gene linked to the occurrence and progression of various illnesses. However, its precise function in gastric cancer (GC) remains unclear.

Nitric oxide synthase 1 (NOS1) has been reported to promote various cancer processes including chemoresistance. However, the role of NOS1 in chemoresistance remained unclear. ATP‑binding cassette, subfamily G, member 2 (ABCG2) identified as a molecular cause multidrug resistance number types, ovarian cancer. The present study observed that cells, expression ABCG2 was significantly upregulated response cis‑diamminedichloroplatinum (cisplatin/DDP) treatment, addition exhibited an increasing...

Hens of a commercial Hy-line brown layer flock in China exhibited increased mortality and decreased egg production at 47 wk age. From to 57 wk, average weekly from 0.11 3.0%, 10 30%, with peak rate (3.0%) observed 54 Necropsy 11 birds demonstrated tissue damage that included hepatitis, liver hemorrhage, rupture, and/or enlarged livers. Microscopic lesions hepatocytic necrosis, lymphocytic periphlebitis, myeloid leukosis. While no bacteria were recovered spleen samples, avian hepatitis E...

Nitric oxide (NO), an important chemical messenger, serves a dual role in tumor progression. synthase isoform 1 (NOS1) was observed to be increasingly expressed various types of cancer, and its expression has been associated with However, the level NOS1 functions human ovarian cancer remain undefined. Using gene profiles from Gene Expression Omnibus (GEO) database, present study revealed that tissues. The investigated effects on vitro cell function, including proliferation, migration...

Telomere length, which is related to chronic diseases and premature mortality, influenced by dietary factors. Zinc known as a antioxidant micronutrient, however, its impact on telomere length remains unclear.We aimed examine the potential effect of zinc intake among middle-aged older individuals in US.Our study included 3,793 US participants aged 45 years from 1999 2002 National Health Nutrition Examination Survey (NHANES). 24-h recall interviews were employed evaluate consumption. Leukocyte...

Postpartum depression (PPD) is considered the most widespread puerperium complication. The associations of major depressive disorder with certain types cerebrovascular diseases and cognitive function have been proposed, but potential causal effects PPD on these phenotypes are still unknown.A Mendelian randomization (MR) research design various methods (e.g., inverse-variance weighted method MR pleiotropy residual sum outlier test) was adopted to establish a relationship between impairment.No...

ABSTRACT Hepatitis E virus (HEV) is the most common cause of acute viral hepatitis worldwide, responsible for approximately 20 million infections annually. Among three open reading frames (ORFs) HEV genome, ORF3 protein involved in release. However, host proteins release need to be clarified. In this study, a protein, thioredoxin domain-containing 5 (TXNDC5), interacted with non-palmitoylated by co-immunoprecipitation analysis. We determined that overexpression or knockdown TXNDC5 positively...

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