A5061613919- Brucella: diagnosis, epidemiology, treatment
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- vaccines and immunoinformatics approaches
- Galectins and Cancer Biology
- Advanced Graph Theory Research
- Artificial Intelligence in Games
- Burkholderia infections and melioidosis
- Escherichia coli research studies
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Trace Elements in Health
- Coastal wetland ecosystem dynamics
- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- Plant responses to water stress
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- RNA and protein synthesis mechanisms
- Interconnection Networks and Systems
- Game Theory and Applications
- Drug Solubulity and Delivery Systems
- Radiation Dose and Imaging
Shihezi University
2018-2025
Sichuan Agricultural University
2023-2024
Huazhong Agricultural University
2020-2021
Xinjiang Production and Construction Corps
2020
National Taipei University of Business
2017
Guangdong Medical College
2017
Linyi People's Hospital
2015
National Taiwan University of Science and Technology
2004-2014
National Chi Nan University
2006-2007
IL-10, an anti-inflammatory cytokine, plays a crucial role in limiting immune responses to pathogens, preventing host damage. However, the mechanisms underlying Brucella-mediated IL-10 production remain incompletely understood. In this study, we demonstrate that proline racemase protein A (PrpA) of Brucella melitensis M5-90 induces macrophages secrete by activating Tpl2-ERK signaling pathway, thereby promoting chronic infection. Moreover, Tpl2 deletion impairs macrophage bactericidal...
Brucellosis is one of the most common zoonotic diseases caused by Brucella spp. However, there currently no vaccine available for humans. Although some attenuated live vaccines have been approved animals, their protective efficacy suboptimal. In previous studies, we utilized an epitope- and structure-based vaccinology platform to identify immunodominant epitopes antigens OMP19, OMP16, OMP25, L7/L12, constructed multi-epitope MEV-Fc against Brucella. this study, was expressed purified via...
The porcine reproductive and respiratory syndrome virus (PRRSV) possesses an inherent ability to adapt environmental transformations undergo evolutionary changes, which has imposed significant economic pressure on the global pig industry. Given potential for recombination among PRRSV genomes variations in pathogenicity, newly emerging isolates are of considerable clinical importance. In this study, we successfully isolated a novel strain named XJ-Z5 from PRRSV-positive samples collected...
The aim of the present study was to determine whether expression levels interleukin (IL)‑2 and IL‑10 may be used as biological markers in Graves' disease (GD) patients. A total 256 individuals, including 118 GD patients 138 healthy were enrolled into study. Blood samples collected from each patient individual, which then subjected enzyme‑linked immunosorbent assay (ELISA). Total RNA proteins determined using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) western blot...
Abstract Background This case report describes the clinical process of a shepherd who suffered brucellosis-related endocarditis (BE) and spondylitis (BS) was infected with Brucella melitensis biovar 3 ( B. 3). Case presentation A 55-year-old male patient admitted to The First Affiliated Hospital Shihezi University on October 11, 2018, due over months intermittent fever, back pain, heart trouble. Rose Bengal Plate test positive, standard agglutination titer for brucellosis 1/800, blood...
Exosomes, membrane vesicles released extracellularly from cells, contain nucleic acids, proteins, lipids and other components, allowing the transfer of material information between cells. Recent studies reported role exosomes in pathogenic microbial infection host immune mechanisms. Brucella-invasive bodies can survive cells for a long time cause chronic infection, which causes tissue damage. Whether are involved anti-Brucella congenital responses has not been reported. Here, we extracted...
Exosomes are small extracellular vesicles that released from cells and function in intercellular communication. Recently, interferon-inducible transmembrane protein 3 (IFITM3) has been identified as a highly effective anti-intracellular pathogen can inhibit the invasion of wide range pathogenic microorganisms. However, whether Brucella infection induces secretion exosomes these contain IFITM3 remain unknown. Here, we focused on immune process infection. This study is first to show melitensis...
Brucellosis, a zoonosis caused by Brucella, is highly detrimental to both humans and animals. Most existing vaccines are live attenuated with safety flaws for people Therefore, it advantageous design multi-epitope subunit vaccine (MEV) prevent Brucella infection. To this end, we applied reverse vaccinology approach. Six cytotoxic T cell (CTL) epitopes, seven helper (HTL) four linear B epitopes from CU/ZN-SOD, Omp31, BP26 were obtained. We linked the CTL, HTL, B-cell appropriate CTB molecular...
Existing Brucella vaccines are attenuated and can cause vaccine-associated brucellosis; these safety concerns have affected their application. Although subunit the advantages of safety, efficacy, low cost, rapid production, they usually poorly immunogenic insufficient to trigger persistent immunity. Therefore, we added layered double hydroxide (LDH) as an adjuvant vaccine formulations enhance immune response antigen.LDH Freund's were combined with outer-membrane vesicles (OMVs)...
Iron is an essential element required for all organisms. response regulator (Irr) a crucial transcriptional and can affect the growth iron uptake of Brucella . The rate melitensis M5-90 irr mutant was significantly lower than that B under normal or iron-sufficient conditions, however, higher iron-limited conditions. In addition, mutation reduced Previous studies suggested Irr protein has multiple target genes in genome are involved metabolism. Therefore, present study, Dap-seq approach used...
Brucella is a genus of Gram-negative intracellular pathogens that cause animal and human diseases. survival replication inside immune cells critical for the establishment chronic infections. Protein modifications by small ubiquitin-related modifier proteins NF-κB pathway are involved in many cellular activities, playing major roles regulating protein function essential pathogenic bacteria during infection. However, relationship between them still largely unknown. We demonstrated abortus 2308...
Brucella abortus is a gram-negative intracellular parasite bacteria that causes serious health hazards in humans and animals. The type IV secretion system (T4SS), encoded by the virB promoter, has been identified as an important virulence factor for abortus, but its impact on A19 remains unclear. In this study, T4SS of was inactivated deletion resulting mutant strain A19ΔvirB. Real-time PCR western blotting analysis demonstrated T4SS-related proteins were not expressed after promoter...
Brucellosis, caused by Brucella spp., is one of the most widespread bacterial zoonoses worldwide. Vaccination still considered best way to control brucellosis. An investigation into differential proteome expression patterns wild and vaccine strains may help researchers clinicians differentiate between diagnose better understand mechanism(s) underlying differences in virulence. In present study, a mass spectrometry-based, label-free relative quantitative proteomics approach was used...
The aim of the present study was to construct a fast-acting, eukaryotic expression vector in cells based on transmembrane-tumor necrosis factor‑α (TM‑TNF‑α) structure. Two types recombinant vectors were constructed, pcDNA3.1-TM-enterokinase-TNF‑α and pcDNA3.1‑TM‑Factor Xa‑TNF‑α, according TNF‑α transmembrane segments. Following generation these vectors, mouse embryonic 3T3 fibroblasts transfected reverse transcription‑polymerase chain reaction western blotting analyses used analyze mTNF‑α...
Abstract Foot-and-mouth disease (FMD) is a pathological caused by the foot- and-mouth virus (FMDV), which mainly affects cloven-hoofed animals. This study was conducted to meta-analysis and experiment on effect of bacteriophages used in development FMDV vaccines. A systematic search for collection protection phage-based vaccine using sensitive strategies. The extracted data were analyzed Rev-Man 5.4 software. T7 phage express capsid protein VP1 OHM-02 strain, recombinant termed OHM-T7....
Introduction Brucella , a type of intracellular Gram-negative bacterium, has unique features and acts as zoonotic pathogen. It can lead to abortion infertility in animals. Eliminating brucellosis becomes very challenging once it spreads among both humans animals, putting heavy burden on livestock people worldwide. Given the increasing spread brucellosis, is crucial develop improved vaccines for susceptible animals reduce disease’s impact. Methods In this study, we effectively used an...
Florfenicol(FF) is an excellent veterinary antibiotic, limited by poor solubility and bioavailability.