A5100323445- Brucella: diagnosis, epidemiology, treatment
- Burkholderia infections and melioidosis
- Galectins and Cancer Biology
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Autophagy in Disease and Therapy
- Atherosclerosis and Cardiovascular Diseases
- Trace Elements in Health
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Advanced Manufacturing and Logistics Optimization
- Mosquito-borne diseases and control
- Animal Diversity and Health Studies
- Viral gastroenteritis research and epidemiology
- Circular RNAs in diseases
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Industrial Technology and Control Systems
- Vibrio bacteria research studies
- Animal health and immunology
- Helminth infection and control
- Fungal Infections and Studies
Shihezi University
2015-2025
Fujian Academy of Agricultural Sciences
2021
Wenzhou Vocational College of Science and Technology
2016
Nanjing Agricultural University
2015
MicroRNA (miRNA) is small non-coding RNA with approximate 22 nt in length.Recent studies indicate that miRNAs play significant roles pathogen-host interactions.Brucella organisms are Gram-negative facultative intracellular bacteria cause Brucellosis.Brucella strains infect macrophages and establish chronic infection by altering host life activities including apoptosis autophagy.Here, we report a comprehensive analysis of miRNA expression profiles mock-and Brucella-infected RAW264.7 cells...
Foot-and-mouth disease virus (FMDV) is an economically devastating viral leading to a substantial loss the swine industry worldwide. A novel alternative strategy develop pigs that are genetically resistant infection. Here, we produce transgenic (TG) constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin (shRNA). In vitro challenge of TG fibroblasts showed shRNA suppressed growth. and non-TG were challenged by intramuscular injection with 100 LD50...
IL-10, an anti-inflammatory cytokine, plays a crucial role in limiting immune responses to pathogens, preventing host damage. However, the mechanisms underlying Brucella-mediated IL-10 production remain incompletely understood. In this study, we demonstrate that proline racemase protein A (PrpA) of Brucella melitensis M5-90 induces macrophages secrete by activating Tpl2-ERK signaling pathway, thereby promoting chronic infection. Moreover, Tpl2 deletion impairs macrophage bactericidal...
Integrating single-cell omics data at an atlas scale enhances our understanding of cell types and disease mechanisms. However, the integration processed by different normalisation methods can lead to biases, such as unexpected batch effects gene expression distortion, leading misinterpretations in downstream analysis. To address these challenges, we present scDenorm, algorithm that reverts normalised raw counts, preserving integrity original measurements ensuring consistent processing during...
This study investigated the role of autophagy in survival invasive bacterium Brucella melitensis strain 16M murine macrophages. Here, was found to trigger autophagosome formation, enhance flux and increase expression level marker protein LC3-II. When pharmacologically inhibited by 3-methyladenine (3-MA), replication efficiency significantly decreased (p < 0.05). These results suggest that favors
Hco-gal-m and -f were two isoforms of galectin cloned from male female Haemonchus contortus, respectively, it was demonstrated that recombinant could act as immune suppressors. However, little is known about the receptors or binding partners these galectins in host. The research molecular mechanisms govern interactions between host molecules will fill a gap our understanding how parasite interact with cells.A yeast two-hybrid system used to identify this research. interaction rHco-gal-m...
Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. Although effective, the current Brucella vaccines (Rev.1 M5-90) have several drawbacks. The first involves residual virulence for animals humans second inability to differentiate natural infection from caused by vaccination. Therefore, melitensis 16M hfq mutant (16MΔhfq) was constructed overcome these Similarly Rev.1 M5-90, 16MΔhfq reduces survival in macrophages mice induces strong protective...
Objectives The underlying mechanism of the inflammatory response against Brucellosis caused by Brucella remains poorly understood. This study aimed to determine role long non-coding RNAs (lncRNAs) in regulating and anti- responses. Materials methods Microarray analysis was performed detect differentially expressed lncRNAs THP-1 cells infected with an S2308 strain. candidate were screened using bioinformatic siRNAs; prediction luciferase reporter assay also conducted, while responses assessed...
Autophagy (or autophagocytosis) is an essential and precise control process by which cells degrade unnecessary or dysfunctional cellular components organelles in the cytoplasm response to nutrient depletion, exogenous pathogens, other stimuli. This results removal of damaged surplus macromolecular complexes via a lysosome-dependent mechanism. Bovine viral diarrhea virus (BVDV) ssRNA Flaviviridae family (genus Pestivirus). BVDV infection major economic losses due poor reproductive performance...
Bovine viral diarrhea-mucosal disease (BVD-MD) is caused by bovine diarrhea virus (BVDV), and results in abortion, stillbirth, fetal malformation cows. Here, we constructed the phage display vector pCANTAB 5E-VHH then transformed it into Escherichia coli TG1-competent cells, to construct an initial anti-BVDV nanobody gene library. We obtained a BVDV-E2 antigen epitope bait protein prokaryotic expression using nucleotide sequence of E2 BVDV-NADL strain published GenBank. Phage was used screen...
MicroRNAs (miRNAs) are small, endogenous, noncoding RNA molecules that serve as powerful regulators of multiple cellular processes, including apoptosis, differentiation, growth, and proliferation. Bovine viral diarrhea virus (BVDV) contributes significantly to health-related economic losses in the beef dairy industries. Although BVDV-induced apoptosis correlates with increased intracellular accumulation bta-miR-29b (miR-29b) expression upregulation Madin-Darby bovine kidney (MDBK) cells...
Outer membrane protein 25 (OMP25), a virulence factor from Brucella, plays an important role in maintaining the structural stability of Brucella. MAPK signal pathway widely exists eukaryotic cells. In present study, human trophoblast cell line HPT-8 and BALB/c mice were infected with Brucella abortus (B. abortus) 2308 strain (S2308) 2308ΔOmp25 mutant strain. The expression cytokines activation mitogen-activated kinase (MAPK) detected. We found that expressions tumor necrosis factor-α...
Context: As a component of the outer membrane in Gram-negative bacteria, lipopolysaccharide (LPS)-induced proliferation and cell cycle progression monocytes/macrophages. It has been suggested that proapoptotic T-cell death-associated gene 51 (TDAG51) might be associated with progression; however, its role interaction between LPS macrophages remains unclear.Objective: We attempted to elucidate role(s) TDAG51 played macrophages.Materials methods: investigated expression RAW264.7 cells...
Brucellosis is a zoonotic disease that poses serious threat to public health and safety. Although the live attenuated vaccines targeting brucellosis, such as M5-90, are effective, there number of drawbacks their use. For example, unable differentiate between natural vaccinated forms infection, these have also been shown cause abortion in pregnant animals. Therefore, safer more potent vaccine required. In present study, B. melitensis 16M TcfSR promoter mutant (16MΔTcfSR) was constructed an...
Abstract The purpose of this study was to investigate whether the Dictyophora echinovolvata spore polysaccharides (DESP) affect immunity in immunocompromised mice induced by cyclophosphamide (CTX). healthy female Kunming were randomly divided into six groups, including a normal control (NC) group, positive model (MC) and three groups treated with low-, intermediate-, high-dose polysaccharide, respectively. A series immunoregulatory properties determined, humoral cellular immunity, immune...
Purpose Reducing production auxiliary time is the key to improve efficiency of existing mixed-flow assembly line. This paper proposes a method combining improved genetic algorithm (GA) and Flexsim software. It also investigates line scheduling just-in-time (JIT) parts feeding scheme reduce waste in while taking hill-drop as an example verify effectiveness method. Design/methodology/approach In this research, presented optimize present The multi-objective mathematical model established based...