A5100745538- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Bioactive natural compounds
- Biological Activity of Diterpenoids and Biflavonoids
- Alkaloids: synthesis and pharmacology
- Traditional and Medicinal Uses of Annonaceae
- Immune Response and Inflammation
- Parkinson's Disease Mechanisms and Treatments
- Natural product bioactivities and synthesis
- Cancer-related molecular mechanisms research
- Biochemical and Structural Characterization
- Wound Healing and Treatments
- Neurological disorders and treatments
- Chemical Synthesis and Analysis
- RNA regulation and disease
- RNA Research and Splicing
- Chemical synthesis and alkaloids
- Antibiotic Resistance in Bacteria
- Nerve injury and regeneration
- RNA modifications and cancer
- Skin Protection and Aging
- Berberine and alkaloids research
- Biological and pharmacological studies of plants
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
Kunming Medical University
2018-2025
Fujian Medical University
2025
Chinese Academy of Sciences
2016-2025
Nanjing University
2024-2025
Beijing Institute of Genomics
2021-2024
Kunming Institute of Zoology
2009-2024
University of Hong Kong
2023-2024
Fifth Hospital In Wuhan
2024
Wuhan University
2023-2024
University of Chinese Academy of Sciences
2014-2024
Abstract Prime editor (PE), which is developed by combining Cas9 nickase and an engineered reverse transcriptase, can mediate all twelve types of base substitutions small insertions or deletions in living cells but its efficiency remains low. Here, we develop spegRNA introducing same-sense mutations at proper positions the reverse-transcription template pegRNA to increase PE’s base-editing up-to 4,976-fold (on-average 353-fold). We also apegRNA altering secondary structure indel-editing...
It is generally agreed that reactive oxygen species (ROS) contribute to skin aging, disorders, and diseases. Skin possesses an extremely efficient antioxidant system. This activity conferred by two systems: enzymes small molecules can scavenge ROS donating electrons. No gene-encoded secreted scavengers have been reported. Amphibian a multifunctional organ acting in defense, respiration, water regulation, although it seems susceptible. skins are easily harmed biological or non-biological...
Long noncoding RNAs (lncRNAs) mediate important epigenetic regulation in a wide range of biological processes and diseases. We applied comprehensive analyses RNA-seq CAGE-seq (cap analysis gene expression sequencing) to characterize the dynamic changes lncRNA rhesus macaque ( Macaca mulatta ) brain four representative age groups. identified 18 anatomically diverse modules 14 mRNA representing spatial, age, sex specificities. Spatiotemporal- sex-biased were generally higher than those...
The advent of base editors (BEs) holds great potential for correcting pathogenic-related point mutations to treat relevant diseases. However, Cas9 nickase (nCas9)-derived BEs lead DNA double-strand breaks, which can trigger unwanted damage response (DDR). Here, we show that the original version catalytically dead Cas12a (dCas12a)-conjugated induce a basal level breaks and minimally activate DDR proteins, including H2AX, ATM, ATR, p53. By fusing dCas12a with engineered human apolipoprotein B...
Prime editors (PEs) were developed to induce versatile edits at a guide-specified genomic locus. With all RNA-guided genome editors, guide-dependent off-target (OT) mutations can occur other sites bearing similarity the intended target. However, whether PEs carry additional risk of guide-independent elicited by their unique enzymatic moiety (i.e., reverse transcriptase) has not been examined systematically in mammalian cells. Here, we cost-effective sensitive platform profile OT effects...
Parkinson's disease (PD) is an age-dependent neurodegenerative that often occurs in those over age 60. Although rodents and small animals have been used widely to model PD investigate its pathology, their short life span makes it difficult assess the aging-related pathology likely occur patient brains. Here, we brain tissues from rhesus monkeys at 2–3, 7–8, >15 years of examine expression Parkin, PINK1, α-synuclein, which are known cause via loss- or gain-of-function mechanisms. We found...
Although cathelicidins in mammals have been well characterized, little is known about the function of cathelicidin amphibians. In present study, a novel 24-residue peptide (cathelicidin-NV, ARGKKECKDDRCRLLMKRGSFSYV) belonging to family was identified from skin plateau frog Nanorana ventripunctata. Cathelicidin-NV showed strong wound healing-promoting activity murine model with full-thickness dermal wound. It directly enhanced proliferation keratinocyte cells, resulting accelerated...
Cathelicidin antimicrobial peptides (mouse, CRAMP; human, LL-37) have broad-spectrum antiviral activities against enveloped viruses, but their mechanisms of action nonenveloped viruses remain to be elucidated. Coxsackievirus B3 (CVB3), a member virus belonging the Enterovirus genus Picornaviridae, is an important pathogen viral myocarditis and dilated cardiomyopathy. Here, we observed that cardiac CRAMP expression was significantly upregulated in mice after CVB3 infection. The administration...
Essentially complete atropdiastereoselectivity was realized in the preparation of biaryl diphosphine dioxide by asymmetric intramolecular Ullmann coupling and oxidative with central-to-axial chirality transfer. A bridged C(2)-symmetric biphenyl phosphine ligand possessing additional chiral centers on linking unit groups synthesized. No resolution step required for enantiomerically pure ligand. These findings offer a general practical tool development previously uninvestigated...
Horseflies are economically important blood-feeding arthropods and also a nuisance for humans vectors filariasis. They rely heavily on the pharmacological properties of their saliva to get blood meal suppress immune reactions hosts. Little information is available antihemostatic substances in horsefly salivary glands; especially no suppressants have been reported. By proteomics or peptidomics coupling transcriptome analysis with testing, several families proteins peptides, which act mainly...
Wound healing-promoting peptides exhibit excellent therapeutic potential in regenerative medicine. However, amphibian-derived wound and their mechanism of action remain to be further elucidated. We hereby characterized a novel peptide, Ot-WHP, derived from Chinese concave-eared frog Odorrana tormota. It efficiently promoted healing mouse model full-thickness wounds. Ot-WHP significantly increased the number neutrophils wounds, modestly neutrophil phagocytosis phorbol myristate acetate...
Abstract Whether direct manipulation of Parkinson’s disease (PD) risk genes in the adult monkey brain can elicit a Parkinsonian phenotype remains an unsolved issue. Here, we used adeno-associated virus serotype 9 (AAV9)-delivered CRISPR/Cas9 system to directly co-edit PINK1 and DJ-1 substantia nigras (SNs) two groups: old group middle-aged group. After operation, exhibited all classic PD symptoms, including bradykinesia, tremor, postural instability, accompanied by key pathological hallmarks...
The roles of bactericidal cathelicidins against bacterial infection have been extensively studied. However, the antibacterial property and mechanism action non-bactericidal are rarely known. Herein, a novel naturally occurring cathelicidin ( Popu CATH) from tree frog Polypedates puerensis ) did not show any direct anti-bacterial activity in vitro. Intriguingly, intraperitoneal injection CATH before inoculation significantly reduced load frogs mice, inflammatory response induced by mice....
Although bactericidal cationic antimicrobial peptides (AMPs) have been well characterized, less information is available about the antibacterial properties and mechanisms of action nonbactericidal AMPs, especially anionic AMPs. Herein, a novel peptide (Gy-CATH) with net charge -4 was identified from skin frog
Many gene-encoded neurotoxins with various functions have been discovered in fish, reptiles, and mammals. A novel 60-residue neurotoxin peptide (anntoxin) that inhibited tetrodotoxin-sensitive (TTX-S) voltage-gated sodium channel (VGSC) was purified characterized from the skin secretions of tree frog Hyla annectans (Jerdon). This is first found amphibians. The IC50 anntoxin for TTX-S about 3.4 μm. Anntoxin shares sequence homology Kunitz-type toxins but contains only two three highly...
Antimicrobial peptides (AMPs) play pivotal roles in protecting against microbial infection fish. However, AMPs from topmouth culter ( Erythroculter ilishaeformis ) are rarely known. In our study, we isolated an AMP the head kidney of culter, which belonged to liver-expressed antimicrobial peptide 2 (LEAP-2) family. Topmouth LEAP-2 showed inhibitory effects on aquatic bacterial growth, including antibiotic-resistant bacteria, with minimal concentration values ranging 18.75 150 μg/ml. It was...
Abstract The first catalytic synthesis of β,γ‐alkynyl α‐amino acid derivatives was achieved by direct addition terminal alkynes to α‐imino esters in the presence an Ag(I) salt under mild reaction conditions.
Ticks are blood-feeding arthropods that may secrete immunosuppressant molecules, which inhibit host inflammatory and immune responses provide survival advantages to pathogens at tick bleeding sites in hosts. In the current work, two families of immunoregulatory peptides, hyalomin-A -B, were first identified from salivary glands hard Hyalomma asiaticum asiaticum. Three copies encoded by an identical gene released same protein precursor. Both -B can exert significant anti-inflammatory...
Chiral bis(sulfonamide)-diamine served as new type of ligand for a Cu(OTf)2-catalyzed asymmetric Friedel–Crafts alkylation reaction indoles with nitroalkenes. The desired products were obtained up to 99% yield and 97% ee.