A5058077800- Nanoplatforms for cancer theranostics
- Ubiquitin and proteasome pathways
- Cervical Cancer and HPV Research
- PARP inhibition in cancer therapy
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Lung Cancer Treatments and Mutations
- RNA modifications and cancer
- Cancer Cells and Metastasis
- Ovarian cancer diagnosis and treatment
- Lung Cancer Research Studies
- Cancer-related Molecular Pathways
- Cancer Research and Treatments
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- Renal cell carcinoma treatment
- Genomics and Chromatin Dynamics
- BRCA gene mutations in cancer
- Epigenetics and DNA Methylation
- Nutrition, Genetics, and Disease
- Uterine Myomas and Treatments
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. - Hans-Knöll-Institut (HKI)
2024
Palacký University Olomouc
2012-2023
University of Birmingham
2023
Washington University in St. Louis
2023
Molecular Partners (Switzerland)
2021-2023
Tbilisi State Medical University
2019-2022
Institute of Molecular and Translational Medicine
2014-2021
Hudson Institute
2019
John Wiley & Sons (United States)
2019
Software based analyses of immunohistochemical staining are designed for obtaining quantitative, reproducible, and objective data. However, often times only a certain type positive cells or structures need to be quantified thus whole image analysis cannot performed. Such an example is Hofbauer placental cells, which show positivity some antigens together with trophoblast, but represent the regions interest (ROIs). Two independent observers evaluated intensity in placenta samples stained...
Abstract The efficacy of antitumor immunity is associated with the metabolic state cytotoxic T cells, which sensitive to tumor microenvironment. Whether ionic signals affect adaptive immune responses unclear. In present study, we show that there an enrichment sodium in solid tumors from patients breast cancer. Sodium chloride (NaCl) enhances activation and effector functions human CD8 + enhanced fitness. These NaCl-induced effects translate into increased cell killing vitro vivo....
The immune microenvironment in breast cancer (BCa) is controlled by a complex network of communication between various cell types. Here, we find that recruitment B lymphocytes to BCa tissues via mechanisms associated with cell-derived extracellular vesicles (CCD-EVs). Gene expression profiling identifies the Liver X receptor (LXR)-dependent transcriptional as key pathway controls both CCD-EVs-induced migration cells and accumulation tissues. increased oxysterol ligands for LXR (i.e.,...
The CD40 receptor is an attractive target for cancer immunotherapy. Although a modest pharmacodynamic effect seen in patients following administration of CD40-targeting monoclonal antibodies (mAb), the doses that could be safely administered do not result meaningful clinical response, most likely due to limited therapeutic window associated with systemic activation. To overcome this issue, we developed multispecific DARPin construct, α-FAPxCD40, which has conditional activity at site...
Background Castration‐resistant prostate cancer (PCa) represents a serious health challenge. Based on mechanistically‐supported rationale we explored new therapeutic options based clinically available drugs with anticancer effects, including inhibitors of PARP1 enzyme (PARPi), and histone deacetylases (vorinostat), respectively, disulfiram (DSF, known as alcohol‐abuse drug Antabuse) its copper‐chelating metabolite CuET that inhibit protein turnover. Methods Drugs their combination ionizing...
Sphingosine 1‑phosphate (S1P) is a bioactive lipid metabolite associated with cancer cell proliferation, survival, migration and regulation of tumor angiogenesis in various cellular animal models. kinase‑1 (SphK1) S1P lyase are the main enzymes that respectively control synthesis degradation S1P. The present study analyzed prognostic predictive value SphK1 expression patients non‑small lung (NSCLC), treated either surgery alone or combination adjuvant carboplatin navelbine. Formalin‑fixed,...
Prostate cancer (PCa) is the second leading cause of cancer-related deaths in men Western countries, and there still an urgent need for a better understanding PCa progression to inspire new treatment strategies. Skp2 substrate-recruiting component E3 ubiquitin ligase complex, whose activity regulated through neddylation. Slug transcriptional repressor involved epithelial-to-mesenchymal transition, which may contribute therapy resistance. Although has previously been associated with...
Primary primitive neuroectodermal tumors (PNETs) are extremely rare in the lung and especially adult women. We describe a case of PNET with aggressive behavior 31-year-old woman. Diagnosis was based on histopathological immunohistochemical studies, confirmed by molecular genetic analysis chromosome rearrangements EWSR1 gene region. Clinical follow-up, post-mortem findings, differential diagnosis also discussed.
An actin-binding protein filamin A connects the actin filament network to cell membrane receptors, and acts as a scaffold for various signaling pathways related cancer growth progression. Recently, it has been reported that is required efficient regulation of early stages DNA repair process. Moreover, some in vitro studies showed overexpression determines resistance cytotoxic drugs, including cisplatin. We aimed analyse expression resected NSCLC (Non Small Cell Lung Cancer) specimens,...
Abstract Calling Cards is a platform technology to record cumulative history of transient protein‐DNA interactions in the genome genetically targeted cell types. The these recovered by next‐generation sequencing. Compared with other genomic assays, readouts which provide snapshot at time harvest, enables correlation historical molecular states eventual outcomes or phenotypes. To achieve this, uses piggyBac transposase insert self‐reporting transposon “Calling Cards” into genome, leaving...
Previously identified as a breast and ovarian cancer susceptibility gene, BRCA1 has gained major scientific interest potential prognostic and/or predictive marker for various tumors, including non-small-cell lung (NSCLC), the leading cause of related mortality worldwide. plays central role in DNA damage response (DDR. It undergoes phosphorylation by DDR kinases at different serine residues, which ser1524 is known to be specifically phosphorylated ATM genotoxic stress.We performed...
Abstract Introduction: Complex structural remodelling of cells and extracellular matrix during cancer initiation progression elicit substantial biomechanical alterations, which can be measured by Atomic Force Microscopy (AFM) at the nanoscale level. Clinical translational evidence indicates that tissue nanomechanical signature (NS) a useful tool for rapid diagnosis, assessment aggressiveness. We demonstrated AFM-based Automated Reliable Tissue Diagnostics (ARTIDIS) platform represents valid...
Abstract Background: Recently, the successful integration of immunotherapy with other treatment modalities has demonstrated its efficacy in enhancing immune activation and suppressing tumors solid cancers. The most promising such efforts include use low dose radiation (LD-XRT) to prime tumor microenvironment enhance T cell infiltration [Barsoumian HB, et al., 2020; Patel RR, 2021; He K, 2023]. With these advancements, there is an urgent need develop clinical biomarkers predict response...
Abstract Background: Neoadjuvant chemotherapy (NACT) is increasingly used in patients with breast cancer. However, predicting response to NACT remains a challenge everyday clinical practice. Increasing (pre)- and evidence demonstrate the importance of cancer biomechanics mediating therapy response. We others have previously demonstrated that complex biomechanical alterations tissues, can be measured by Atomic Force Microscopy (AFM) at nanoscale level. Incorporating AFM measurements biopsies...
Abstract Background: The conventional diagnosis of breast cancer followed by molecular subtyping for therapy selection is a lengthy process and associated with patient clinician anxiety. Efficient, bedside approaches are desirable to drive fast early planning clinical management. This trial (NCT06085833) tackles the novel concept automated reliable tissue diagnostics (ARTIDIS) based on measurement nanomechanical signature fresh biopsies from patients lesions. study our previous single center...
Abstract Background: With the recent advancements of spatial omics, it has become increasingly recognized that analysis tumor architecture is key to decipher heterogenous intratumoral relationships between different components and provide better understanding cancer therapy resistance, as well help identify potential targets for personalized therapy. The challenge discovery implementation such biomarkers still lays in technological methodological difficulties, their translation into clinical...