A5057282895- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune cells in cancer
- Reproductive System and Pregnancy
- Inflammasome and immune disorders
- Immune Response and Inflammation
- Antifungal resistance and susceptibility
- Single-cell and spatial transcriptomics
- Endometriosis Research and Treatment
- Glycosylation and Glycoproteins Research
- S100 Proteins and Annexins
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Retinal Diseases and Treatments
- Hematological disorders and diagnostics
- Fibroblast Growth Factor Research
- Acute Ischemic Stroke Management
- Seed and Plant Biochemistry
- Angiogenesis and VEGF in Cancer
- Autoimmune and Inflammatory Disorders Research
- Extracellular vesicles in disease
- Atherosclerosis and Cardiovascular Diseases
- Autophagy in Disease and Therapy
- Cell Adhesion Molecules Research
- Endometrial and Cervical Cancer Treatments
- Cerebral Venous Sinus Thrombosis
Harvard University
2019-2025
Institute of Hematology & Blood Diseases Hospital
2019-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2019-2025
Sichuan Agricultural University
2025
Dana-Farber/Harvard Cancer Center
2019-2025
Mass General Brigham
2025
Chengdu University of Traditional Chinese Medicine
2025
Boston Children's Hospital
2019-2024
Brigham and Women's Hospital
2024
Dana-Farber/Boston Children's Cancer and Blood Disorders Center
2024
Plasma membrane perforation elicited by caspase cleavage of the gasdermin D (GSDMD) N-terminal domain (GSDMD-NT) triggers pyroptosis. The mechanisms underlying GSDMD translocation and pore formation are not fully understood. Here, using a proteomic approach, we identified fatty acid synthase (FASN) as GSDMD-binding partner. S-palmitoylation at Cys
Candida albicans is the most common cause of fungal sepsis. Inhibition inflammasome activity confers resistance to polymicrobial and LPS-induced sepsis; however, signaling appears protect against C. infection, so inhibitors are not clinically useful for candidiasis. Here we show disruption GSDMD, a known target key pyroptotic cell death mediator, paradoxically alleviates candidiasis, improving outcomes survival Candida-infected mice. Mechanistically, hijacked canonical inflammasome-GSDMD...
Abstract Both lytic and apoptotic cell death remove senescent damaged cells in living organisms. However, they elicit contrasting pro- anti-inflammatory responses, respectively. The precise cellular mechanism that governs the choice between these two modes of remains incompletely understood. Here we identify Gasdermin E (GSDME) as a master switch for neutrophil pyroptotic death. tightly regulated GSDME cleavage activation aging neutrophils are mediated by proteinase-3 caspase-3, leading to...
Significance Immunotherapy benefits some aggressive breast cancers, but many tumors do not respond to checkpoint blockade. Novel strategies increase cancer immunogenicity are needed improve immunotherapy. Here, we used epithelial cell adhesion molecule (EpCAM) aptamer-linked small-interfering RNA chimeras (AsiC) selectively knock down genes in mouse cancers induce tumor neoantigens or overcome immune evasion. Individual gene knockdown markedly delayed growth and enhanced antitumor immunity....
Diabetes mellitus is considered to be a major risk factor for cardiovascular disease, the most common cause of death in diabetes. However, therapeutic strategies myocardial protection patients with diabetes are still limited. Cordycepin traditional Tibetan medicine long history widespread use, and exerts wide range anti-tumor, anti-inflammatory, anti-oxidative effects. In recent years, although potential cordycepin has attracted attention researchers, it remains unknown whether plays...
Activated natural killer (NK) cells proliferate in large numbers murine mesometrial endometrium from Day 6 to 12 of gestation (term = 19 days) become the most abundant uterine lymphocytes. Early human decidua contains analogous CD56+/CD16- cells. Murine (u)NK localize basalis and lymphoid aggregate pregnancy (MLAp). Decidua MLAp are transient, pregnancy-associated tissues traversed by maternal arteries placentas. Uterine NK sensitize these arteries, facilitating their structural changes into...
BackgroundPathological neovascularization in neovascular age-related macular degeneration (nAMD) is the leading cause of vision loss elderly. Increasing evidence shows that cells myeloid lineage play important roles controlling pathological endothelium formation. Suppressor cytokine signaling 3 (SOCS3) pathway has been linked to neovascularization.MethodsWe utilised a laser-induced choroidal (CNV) mouse model investigate aspect human AMD. In several cell reporter mice, bone marrow chimeric...
Wound healing is related to proliferation, migration, and angiogenesis of keratinocytes. Insulin-like growth factor 2 mRNA binding protein (IGF2BP2) an important N6-methyladenosine (m6A) reader, which involved in multiple processes, including wound healing. However, the function mechanism IGF2BP2 keratinocyte processes are largely uncertain. In present study, expression levels heparanase (HPSE) were detected by quantitative reverse transcription polymerase chain reaction western blotting...
Problem: Dynamic changes occur in endometrial immune cell populations during pregnancy but regulatory events promoting recruitment to the uterus are not established. Ovarian steroid hormones promote l ‐selectin and α 4‐integrin‐mediated interactions between human peripheral natural killer (NK) cells uterine endothelium while CXCR3, CXCR4 their ligands implicated homing of uNK decidua. Method study: Mice genetically‐ablated for or CXCR3 were studied. Morphometric analyses implantation sites...
Abstract In addition to Ag recognition, some Abs are capable of killing target organisms in the absence phagocytes and complement. this study, we report that an anti-Pseudomonas aeruginosa O6ad LPS IgG1, tobacco-expressed human S20 IgG1 (te-hS20), as well its recombinant Fab single-chain variable fragment (scFv) fragments have cellular- complement-independent bactericidal activity. te-hS20 scFv significantly reduced viability P. dose- time-dependent manners vitro also showed lower levels...
Summary The full neutrophil heterogeneity and differentiation landscape remains incompletely characterized. Here we profiled >25,000 differentiating mature mouse neutrophils using single-cell RNA sequencing to provide a comprehensive transcriptional of maturation, function, fate decision in their steady state during bacterial infection. Eight populations were defined by distinct molecular signatures. three peripheral blood subsets arise from maturing bone marrow subsets. Driven both known...
The MRP8-Cre-ires/EGFP transgenic mouse (Mrp8creTg, on C57BL/6J genetic background) is popular in immunological and hematological research for specifically expressing Cre recombinase an EGFP reporter neutrophils. It often crossed with other lines carrying loxP-flanked genes to achieve restricted gene knockout However, due the way which line was created, basic knowledge about transgene host genome, such as its integration site(s) flanking sequences, remains largely unknown, hampering robust...
Decidualization of the mouse and human uterus is accompanied by influx large numbers natural killer lymphocytes (uterine [uNK] cells). Adoptive cell transfer to mated, alymphoid mice a general model suitable for analysis homing, differentiation function uNK lineage. Simultaneous two populations, tagged with different fluorescent tracker dyes, permits in vivo key mechanisms regulating lymphocyte homing described. cells are central initiation spiral arterial modification (i.e., structural...
Emergency myelopoiesis (EM) represents a general hematopoietic response to infection, but how infection frequency and duration are sensed is unknown. Here we describe IL1R1-mediated trained program, termed emergency (TEM), in recurring E.coli sustained C. albicans infection. IL1R1 upregulation, triggered by primary via TLR2 specific BM stem progenitor cells, formed an intrinsic transplantable short-lived hematological memory that contributed IL-1 signaling upregulation reinforced...
Background: Pathological neovascularization in neovascular age-related macular degeneration (nAMD) is the leading cause of vision loss elderly. Increasing evidence shows that cells myeloid lineage play important roles controlling pathological endothelium formation. SOCS3 pathway has been linked to neovascularization. Methods: We utilized a laser-induced CNV mouse model investigate aspect human AMD. In several cell reporter mice, bone marrow chimeric mice and Socs3 loss-of-function (knockout)...