A5059292272- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Cell Image Analysis Techniques
- Nicotinic Acetylcholine Receptors Study
- Herpesvirus Infections and Treatments
- Retinal Development and Disorders
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Virus-based gene therapy research
- Advanced Fluorescence Microscopy Techniques
- Mosquito-borne diseases and control
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Neuroendocrine regulation and behavior
- Neuroscience and Neural Engineering
- Neurotransmitter Receptor Influence on Behavior
- Circadian rhythm and melatonin
- Pain Mechanisms and Treatments
- Neuroscience of respiration and sleep
- Olfactory and Sensory Function Studies
- Glaucoma and retinal disorders
- Transgenic Plants and Applications
- Protein Kinase Regulation and GTPase Signaling
- Connexins and lens biology
Chinese Academy of Sciences
1999-2023
Shenzhen Institutes of Advanced Technology
2019-2023
National Medical Products Administration
2023
Wuhan Institute of Physics and Mathematics
2013-2022
State Key Laboratory of Magnetism
2022
University of Chinese Academy of Sciences
2019-2022
Suzhou Research Institute
2019-2020
Center for Excellence in Brain Science and Intelligence Technology
2017-2018
Huazhong University of Science and Technology
2018
Tufts University
2014
The lateral entorhinal cortex (LEC) receives direct input from olfactory bulb mitral cells and piriform cortical pyramidal is the gateway for to hippocampus. However, LEC also projects back bulb. Activity in shaped by perirhinal cortices, hippocampus, amygdala, thus could provide a rich contextual modulation of odor processing. present study further explored feedback anterior examining how top-down modulates evoked activity rats. Retrograde viral tracing confirmed projections both cortices....
Abstract Different emotional states lead to distinct behavioural consequences even when faced with the same challenging events. Emotions affect learning and memory capacities, but underlying neurobiological mechanisms remain elusive. Here we establish models of learned helplessness (LHL) hopefulness (LHF) by exposing animals inescapable foot shocks or anticipated avoidance trainings. The LHF show spatial potentiation excitatory monosynaptic upscaling between posterior basolateral amygdale...
Tissue optical clearing enables imaging deeper in large volumes with high-resolution. Clear T2 is a relatively rapid method no use of solvents or detergents, hence poses great advantage on preservation diverse fluorescent labels. However, this suffers from insufficient tissue transparency, especially for adult mouse brain blocks. In work, we develop and versatile based , termed RTF (Rapid Triethanolamine Formamide), aiming better capability. The results show that can not only efficiently...
The CA1, an important subregion of the hippocampus, is anatomically and functionally heterogeneous in dorsal ventral hippocampus. Here, to dissect distinctions between (dCA1) CA1 (vCA1) anatomical connections, we systematically analyzed direct inputs dCA1 vCA1 projection neurons (PNs) with rabies virus-mediated retrograde trans-monosynaptic tracing system Thy1-Cre mice. Our mapping results revealed that input proportions distributions PNs varied significantly. Inside hippocampal region,...
Hierarchy in sensory processing is reflected by the innervation balance on cortical interneurons corticothalamic network.
Aversive stimuli can impact motivation and support associative learning as reinforcers. However, the neural circuitry underlying processing of aversive reinforcers has not been elucidated. Here, we report that a subpopulation central amygdala (CeA) GABAergic neurons expressing protein kinase C-delta (PKC-δ+) displays robust responses to during negative reinforcement learning. Importantly, projections from PKC-δ+ CeA substantia innominata (SI) could bi-directionally modulate Moreover,...
Abstract The amygdala, one of the most studied brain structures, integrates brain-wide heterogeneous inputs and governs multidimensional outputs to control diverse behaviors central survival, yet how amygdalar input-output neuronal circuits are organized remains unclear. Using a simplified cell-type- projection-specific retrograde transsynaptic tracing technique, we scrutinized afferent four major output groups in basolateral complex (BLA) that project bed nucleus stria terminals (BNST),...
The basal forebrain cholinergic system (BFCS) robustly modulates many important behaviors, such as arousal, attention, learning and memory, through heavy projections to cortex hippocampus. However, the presynaptic partners governing BFCS activity still remain poorly understood. Here, we utilized a recently developed rabies virus-based cell-type-specific retrograde tracing map whole-brain afferent inputs of BFCS. We found that receives from multiple cortical areas, orbital frontal cortex,...
Nociceptive signals are usually transmitted to layer 4 neurons in somatosensory cortex via the spinothalamic-thalamocortical pathway. The 5 corticospinal sensorimotor reported receive output of superficial layers; and their descending axons innervate spinal cord regulate basic functions. Here, we show that a subset receives inputs through direct spino-cortical circuit bypassing thalamus, thus define these as recipient (SCRNs). Morphological studies revealed branches from ascending formed...
s: The mammalian basal forebrain (BF), a heterogenous structure providing the primary cholinergic inputs to cortical and limbic structures, plays crucial role in various physiological processes such as learning/memory attention. Despite involvement of BF neurons (BFCNs) olfaction related memory has been reported, underlying neural circuits remain poorly understood. Here, we combined viral trans-synaptic tracing systems ChAT-cre transgenic mice systematically reveal relationship between...
Neurotropic viral transsynaptic tracing is an increasingly powerful technique for dissecting the structure and function of neural circuits. Herpes simplex virus type 1 strain H129 has been widely used as anterograde tracer. However, HSV tracers still have several shortcomings, including high toxicity, low sensitivity non-specific retrograde labeling. Here, we aimed to construct high-brightness by increasing expression exogenous genes carried viruses. Using a Trojan horse-like strategy,...
Abstract Background Abnormal tau accumulation and cholinergic degeneration are hallmark pathologies in the brains of patients with Alzheimer’s disease (AD). However, sensitivity neurons to AD-like strategies ameliorate tau-disrupted spatial memory terms neural circuits still remain elusive. Methods To investigate effect mechanism circuit Alzheimer's disease-related hippocampal memory, overexpression human wild-type Tau (hTau) medial septum (MS)-hippocampus (HP) was achieved by specifically...
ABSTRACT Mapping the detailed cell-type-specific input networks and neuronal projectomes are essential to understand brain function in normal pathological states. However, several properties of current tracing systems, including labeling sensitivity, trans-synaptic efficiencies, reproducibility among different individuals Cre-driver animals, still remained unsatisfactory. Here, we developed MAP-ENVIVIDERS, a recombinase system-dependent vector mixing-based strategy for highly efficient...
Self-injurious behavior (SIB) is commonly observed in patients with neuropsychiatric disorders, as well nonclinical populations stress-related mental-health problems. However, the exact circuitry mechanisms underlying SIB have remained poorly understood. Here, bilateral injection of muscimol into entopeduncular nucleus (EP), we established a rat model SIB. Following injection, male rats exhibited dose-dependent manner stereotypic self-biting that lasted for hours and often resulted wounds...