Myeloperoxidase (MPO) is an oxidative stress biomarker, with elevated MPO levels linked to chronic kidney disease (CKD) progression. Metal exposure a risk factor for CKD, and also correlated expression, specific genotypes expression. Therefore, we examined whether plasma or polymorphisms were explored these factors modified associations between CKD metal concentrations. Accordingly, recruited 395 age- sex-matched controls 215 patients (persistent estimated glomerular filtration rate (eGFR) <...

The transient receptor potential type 5 (TRPV5) channel is present in kidney and intestine important for transepithelial (re)absorption of calcium these tissues. We report that whole-cell patch clamp recording extracellular acidification inhibited rabbit TRPV5 with apparent pKa ∼6.55. two loops between the fifth sixth transmembrane segments presumably form part outer opening pore likely are binding regulation by external protons. found mutation glutamate 522 to glutamine (E522Q) decreased...

Abstract Ischemia-reperfusion (I/R) induced acute kidney injury (AKI) is regulated by transcriptional factors and microRNAs (miRs). However, modulation of miRs has not been characterized in AKI. Here, we found that urinary miR-16 was 100-fold higher AKI patients. MiR-16 detected earlier than creatinine mouse after I/R. Using TargetScan, the 3′UTR B-cell lymphoma 2 (BCL-2) complementary to decrease fluorescent reporter activity. Overexpression mice significantly attenuated renal function...

Abstract Billions of people have obesity-related metabolic syndromes such as diabetes and hyperlipidemia. Promoting the browning white adipose tissue has been suggested a potential strategy, but drug still needs to be identified. Here, genetic deletion activating transcription factor 3 ( ATF3 −/− ) in mice under high-fat diet (HFD) resulted obesity insulin resistance, which was abrogated by virus-mediated restoration. ST32da, synthetic inducer isolated from Salvia miltiorrhiza , promoted...

Prostacyclin and peroxisome proliferator-activated receptors (PPAR) protect against ischemia-reperfusion (I/R) injury by the induction of an anti-inflammatory pathway. In this study, we examined prostacyclin-enhanced protective effect PPARalpha in I/R-induced kidney injury. PPAR-alpha reduced NF-kappaB-induced overexpression TNF-alpha apoptosis cultured cells. a murine model, pretreating wild-type (WT) mice with activator, docosahexaenoic acid (DHA), significantly renal dysfunction (lowered...

Activating transcription factor 3 (ATF3), a cAMP response element-binding protein/ATF family factors member, has been implicated in the cardiovascular and inflammatory system is rapidly induced by ischemic-reperfusion injuries. We performed transverse aortic banding (TAB) experiments using ATF3 gene-deleted mice (ATF3^−/−) wild-type (WT) to determine what effect it might have on heart failure pressure overloading. Compared with WT mice, ATF3^−/− were found...

Chronic kidney disease (CKD) is inflammation-related. Patients with chronic renal failure who undergo hemodialysis (HD) have some acute adverse effects caused by dialysis-induced oxidative stress, protein adsorption, platelet adhesion, and activation of coagulation inflammation. Here, resonantly illuminated gold nanoparticles-modified artificial (AuNPs@AK) for achieving high efficiency accompanying therapeutic strategy CKD during HD proposed. The in removing uremic toxins increased...

Lipopolysaccharide (LPS) triggers innate immunity mainly via TLR4 signaling. ATF3 is a negative regulator of HMGB1 plays critical role in the final step sepsis. However, mechanisms and regulating immunity-induced sepsis are incompletely understood. In this study, we found that serum levels were 10-fold higher patients with than normal controls. We further demonstrated gene knockout mice subjected to LPS-induced endotoxemia correlates an increase mortality rate elevated expression IL-6, TNF-...

Uremic toxins are mainly represented by blood urine nitrogen (BUN) and creatinine (Crea) whose removal is critically important in hemodialysis (HD) for kidney disease. Patients undergoing HD have a complex illness, resulting from: inadequate of organic waste, dialysis-induced oxidative stress membrane-induced inflammation. Here we report innovative breakthroughs efficient safe using plasmon-induced dialysate comprising Au nanoparticles (NPs)-treated (AuNT) water that distinguishable from...

Diagnosis of acute kidney injury by detecting urinary miRNA using capped gold nanoslit SPR in a microfluidic chip.

The clinical assessment of short-stranded nucleic acid biomarkers such as miRNAs could potentially provide useful information for monitoring disease progression, prompting definitive treatment decisions. In the past decade, advancements in biosensing technology have led to a shift towards rapid, real-time and label-free detection systems; such, surface plasmon resonance (SPR) biosensor-based has become high interest. Here, we developed an automated multiplex transmissive (t-SPR) platform...

Peroxisome proliferator-activated receptor (PPAR)-<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math>, a member of large nuclear superfamily, plays major role in the regulation lipid metabolism. Recently, PPAR<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mi>α</mml:mi></mml:math>activation has been shown to confer additional benefits on endothelial function, kidney and anti-inflammation, suggesting that...

Pharmacological studies indicate that Salvia miltiorrhiza extract (SME) can improve cardiac and blood vessel function. However, there is limited knowledge regarding the effects (exerted through epigenetic regulation) of SME newly derived single compounds, with exception tanshinone IIA IB, on obesity-induced metabolic disorders. In this study, we administered or dimethyl sulfoxide (DMSO) as controls to male C57BL/J6 mice after they were fed a high-fat diet (HFD) for 4 weeks. treatment...

The aim of this study was to explore the molecular mechanisms underlying protective effect pravastatin against carboplatin-induced nephrotoxicity in rodents. We exposed rat NRK-52E renal tubular epithelial cells carboplatin, with or without pravastatin. Pravastatin decreased production reactive oxygen species, increased expression heme oxygenase-1 (HO-1), cyclooxygenase-2, and 6-keto prostaglandin F1α, enhanced nuclear translocation peroxisome proliferator-activated receptor-α (PPARα), HO-1...

Adiponectin is a circulating adipose-derived cytokine that may act as an antioxidative and anti-inflammatory protein. Although adiponectin has been reported to exert cytoprotective effects in acute cardiac diseases, its on chronic heart failure are less clear. Therefore, we aimed investigate whether would have beneficial effect iron-induced elucidate regulation cardiomyocytes. Mice were first treated with iron dextran for 4 weeks induce iron-overload cardiomyopathy. They exhibited decreased...

Background: Pain is one of the most common symptoms experienced by patients with endstage renal disease.Although NSAIDs may lead to adverse events, NSAID use appears be considerably high in end-stage disease.However, whether associated an increased risk mortality this population remains unknown.Aim: This study aimed investigate association between and disease.Patients methods: We used population-based Taiwan National Health Insurance Research Database disease receiving dialysis.A total 3,383...

This study hypothesized that plasma folate and vitamin B12 levels modified the association between blood lead cadmium total urinary arsenic bone loss. A of 447 subjects who received a physical examination at Wanfang Hospital Medical Center were recruited. Bone loss was defined as calcaneus mineral density T-score less than -1. Blood concentrations measured by ICP-MS. Urinary species determined using HPLC-HG-AAS. SimulTRAC-SNB radioassay used to measure folate, B12, homocysteine levels. Total...

Background: Recent studies demonstrated that iron overload could enhance the production of arachidonic acid and prostanoid, suggesting a causal connection between these signals iron-overload cardiomyopathy. However, information regarding downstream signaling is limited. Because thromboxane A2 (TXA2) prostacyclin are 2 major prostanoids in cardiovascular system, TXA2 plays role vascular atherosclerosis has pro-inflammatory characteristics, we intended to elucidate Methods Results: A 4-week...

Predicting mortality in dialysis patients based on low intact parathyroid hormone levels is difficult, because aluminum intoxication, malnutrition, older age, race, diabetes, or peritoneal may influence these levels. We investigated the clinical implications of relation to using sensitive, stratified, and adjusted models a nationwide database. analyzed data from 2005 2012 that were held Taiwan Renal Registry Data System, 94,983 hemodialysis with valid regarding their included this study. The...

Previous studies show that serum bilirubin has potent antioxidant effect and is associated with protection from kidney damage reduce cardiovascular events. The aim of this study was to examine the association total level mortality in uremia patients who underwent hemodialysis.This a nationwide retrospective long-term cohort study. Patients were registered Taiwan Renal Registry Data System (TWRDS) 2005 2012. A 115,535 hemodialysis surveyed those valid baseline (TB) data enrolled. All-cause...

Sepsis-induced lung injury is a lethal complication with no effective treatment options, affecting millions of people worldwide. Oroxylin A (OroA) natural flavonoid potent anticancer effects, but its modulating effect on inflammation through microRNAs (miRs) not apparent. In this report, we investigated the target genes miR pathway mediated by OroA and assessed potential for novel treatments septic injury. An array screening quantitative polymerase chain reaction identified that miR-155-5p...

Studies on the association of total cholesterol (TC) levels and mortality in hemodialysis (HD) patients demonstrated conflicting results. The differenct effect Hypercholesterolemia HD based presence myocardial infarction (MI) or coronary artery disease (CAD) is unknown.We analyzed data from Taiwan Renal Registry Data System (TWRDS) between 2005 2012. Patients were divided into MI/CAD non-MI/CAD group. primary outcome was three-year mortality. first year average TC change level third dialysis...