A5100752547- Ion Transport and Channel Regulation
- Caveolin-1 and cellular processes
- Pluripotent Stem Cells Research
- Immune cells in cancer
- Magnesium in Health and Disease
- Peroxisome Proliferator-Activated Receptors
- CRISPR and Genetic Engineering
- Electrospun Nanofibers in Biomedical Applications
- Cancer, Hypoxia, and Metabolism
- Bone Metabolism and Diseases
- Pancreatitis Pathology and Treatment
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Cancer, Lipids, and Metabolism
- Atherosclerosis and Cardiovascular Diseases
- Bone health and osteoporosis research
- Bone health and treatments
- Renal and related cancers
- Dyeing and Modifying Textile Fibers
- Adipokines, Inflammation, and Metabolic Diseases
- Phagocytosis and Immune Regulation
- Fibroblast Growth Factor Research
- Advanced Glycation End Products research
- Ferroptosis and cancer prognosis
- Cell Image Analysis Techniques
Versiti Blood Center of Wisconsin
2020-2025
Sichuan University
2025
West China Hospital of Sichuan University
2025
Guangzhou University of Chinese Medicine
2025
Foshan Hospital of TCM
2025
Peking University
2024
Center for Interdisciplinary Studies
2024
Morgridge Institute for Research
2013-2024
Medical College of Wisconsin
2024
Nanjing Medical University
2013-2022
Human pluripotent stem cells offer the best available model to study underlying cellular and molecular mechanisms of human embryonic lineage specification. However, it is not fully understood how individual exit state transition towards their respective progenitor states. Here, we analyze transcriptomes cell-derived lineage-specific progenitors by single-cell RNA-sequencing (scRNA-seq). We identify a definitive endoderm (DE) transcriptomic signature that leads us pinpoint critical time...
Rationale: A hallmark of chronic inflammatory disorders is persistence proinflammatory macrophages in diseased tissues. In atherosclerosis, this associated with dyslipidemia and oxidative stress, but mechanisms linking these phenomena to macrophage activation remain incompletely understood. Objective: To investigate dyslipidemia, through modulation immunometabolism explore therapeutic potential targeting specific metabolic pathways. Methods Results: Using a combination biochemical,...
Helicobacter pylori (H. pylori) infection was identified as a substantial risk factor for gastric cancer development, but the eradication of H. did not necessarily lead to reduction in incidence cancer. Non-Helicobacter (non-H. bacteria stomach are involved transformation gastritis carcinoma. The aim this study characterize microbiome composition mucosa and its functions non-H. pylori-negative) patients with chronic atrophic (CAG) non-atrophic (CNAG). Fourteen CNAG samples twenty-three CAG...
Fibroblast growth factor receptor 2 (Fgfr2) signaling is critical in maintaining ureteric branching architecture and mesenchymal stromal morphogenesis the kidney. substrate 2α (Frs2α) a major docking protein for Fgfr2 with downstream targets including Ets variant (Etv) 4 Etv5 other systems. Furthermore, global deletion of Frs2α causes early embryonic lethality. The purpose study was to determine role mediating epithelium. To that end, we generated mice conditional epithelium ( UB−/− ) point...
Contractile to synthetic phenotypic switching of smooth muscle cells (SMCs) contributes stenosis in vascular disease and transplants. To generate more contractile SMCs, we performed a high-throughput differentiation screen using MYH11-NLuc-tdTomato human embryonic stem cell reporter line. We identified RepSox as factor that promotes MYH11-positive by promoting NOTCH signaling. induces SMCs exhibit phenotype than generated PDGF-BB TGF-β1, two factors previously used for SMC but which also...
Objective: Metastasis and therapeutic resistance are the major determinants of lung cancer progression high mortality. Epithelial-mesenchymal transition (EMT) plays a key role in metastasis resistance. Highly expressed glucose-regulated protein 78 (GRP78) is poor prognostic factor possibly correlated with EMT. This study aims to examine whether up-regulation GRP78 involved EMT adenocarcinoma explore underlying downstream molecular pathways. Study Design: was assessed by analysis cell...
Of the four Na-K-ATPase α-isoforms, ubiquitous α1 possesses both ion transport and Src-dependent signaling functions. Mechanistically, we have identified two putative pairs of domain interactions between Src that are critical for function. Our subsequent report α2 lacks these Src-binding sites fails to carry on further supported our proposed model direct interaction but fell short providing evidence a causative role. This hypothesis was specifically tested here by introducing key residues...
Hypoimmune gene edited human pluripotent stem cells (hPSCs) are a promising platform for developing reparative cellular therapies that evade immune rejection. Existing first-generation hypoimmune strategies have used CRISPR/Cas9 editing to modulate genes associated with adaptive (e.g., T cell) responses, but largely not addressed the innate monocytes, neutrophils) mediate inflammation and rejection processes occurring early after graft transplantation. We identified adhesion molecule ICAM-1...
Recent studies have highlighted a critical role for CD40 in the pathogenesis of renal injury and fibrosis. However, little is currently understood about regulation this setting.We use novel Na/K-ATPase cell lines inhibitors order to demonstrate regulatory function with regards expression function. We utilize 5/6 partial nephrectomy as well direct infusion ligand mechanism exists vivo.We that knockdown α1 isoform causes reduction while rescue but not α2 restores epithelial cells. Second,...
We have reported that the reduction in plasma membrane cholesterol could decrease cellular Na/K-ATPase α1-expression through a Src-dependent pathway. However, it is unclear whether regulate other α-isoforms and molecular mechanisms of this regulation are not fully understood. Here we used cells expressing different α isoforms found by U18666A decreased expression α1-isoform but α2- or α3-isoform. Imaging analyses showed redistribution α1 α3 α2. Moreover, led to late endosomes/lysosomes,...
Several signaling events have been recognized as essential for regulating cell lineage specification and organogenesis in animals. We find that the gain of an amino-terminal caveolin binding motif (CBM) α subunit Na/K-adenosine triphosphatase (ATPase) (NKA) is required early stages both mice Caenorhabditis elegans. The evolutionary CBM occurred at same time acquisition sites Na+/K+. Loss this does not affect or initiation organogenesis, but arrests further organ development. Mechanistically,...
A Na/K-ATPase α1 caveolin-binding motif regulates adipogenesis. Mutation of this binding in the mouse leads to reduced fat with increased extracellular matrix production and inflammation. RNA-seq analysis pharmacological interventions human iPSC-derived adipocytes revealed that TGF-β signal, rather than Na/K-ATPase-mediated ion transport, is a key mediator NKA regulation
In the present study, we investigated whether high dietary Ca and exogenous parathyroid hormone 1–34 fragments (PTH 1–34) have synergistic effects on bone formation in adult mice, explored related mechanisms. Adult male mice were fed a normal diet, high-Ca PTH-treated or diet combined with subcutaneously injected PTH (80 μg/kg per d) for 4 weeks. Bone mineral density, trabecular volume, osteoblast number, alkaline phosphatase (ALP)- type I collagen-positive areas, expression levels of...