To study the regulatory relationship between ozone-induced mitophagy and pyroptosis in lung epithelial cells.First, type I primary alveolar cells male Wistar rats were treated with ozone at different dosages. The ATP content mitochondrial membrane potential significantly decreased cells. mitophagy-related markers PINK1/Parkin pathway-related proteins, co-localization of LC3, Parkin, mitochondria indicated that exposure triggered mitophagy. On other hand, reactive oxygen species (ROS)...

Oxidative stress is one of the main causes myocardial injury, which associated with cardiomyocyte death. Mitochondria play a key role in triggering necrosis and apoptosis pathway cardiomyocytes under oxidative stress. Although prohibitin (PHB) has been acknowledged as mitochondrial chaperone, its functions are poorly characterized. The present research was designed to investigate cardioprotective PHB mitochondria. can increase content mitochondria time-dependent manner. Overexpression...

Although bone morphogenetic protein 9 (BMP9) is highly capable of promoting the osteogenic differentiation mesenchymal stem cells (MSCs) both in vitro and vivo, molecular mechanisms involved remain to be fully elucidated. Runt-related transcription factor (RUNX)3 an essential regulator osteoblast/chondrocyte maturation. However, exact role RUNX3 BMP9 osteoinductive activity unknown. In this study, we sought investigate functional BMP9-induced MSCs. We found that upregulated endogenous...

Four new phenolic compounds, hemerodiphenylethanes A-D (1-4), along with eight known compounds methyl 2,4-dihydroxy-6-[2-(4-hydroxyphenyl) ethyl] benzoate (5), 2,4-dihydroxy-6-(4-hydroxyphenethyl)-3-methylbenzoate (6), neochlorogenic acid (7), isoquercetin (8), isorhamnetin-3-O-β-D-galactoside (9), velutin (10), rutin (11), and (+)-syringaresinol (12), were isolated from the stems leaves of Hemerocallis citrina Baroni (Daylily). Their structures elucidated based on HR-ESI-MS, 1D- 2D-NMR...

As one of the least studied bone morphogenetic proteins (BMPs), BMP9 is highly capable promoting osteogenic differentiation. However, underlying mechanism involved remains largely unknown. Recent studies have demonstrated that RUNX1 (runt-related transcription factor 1) essential in osteoblast/chondrocyte maturation. In this study, we investigated function BMP9-induced murine mesenchymal stem cell line (C3H10T1/2) and multi-lineage lines (C2C12 MEFs). Our data showed promoted endogenous...

Abstract Mesenchymal stem cells (MSCs) are multipotent progenitors that can differentiate into a variety of cell types under proper stimuli. Bone morphogenetic protein 9 (BMP9) is able to simultaneously induce both adipogenic and osteogenic differentiation MSCs although the regulatory molecules involved remain be fully identified characterized. Heme oxygenase 1 (Hmox1) plays an essential role not only in fat metabolism, but also bone development. In present study, we investigated functional...

Chemical examination of the methanolic extract from stem bark Daphne aurantiaca led to isolation three new flavonoids (1-3), and 29 known flavonoids. All 32 compounds were isolated for first time aurantiaca. The isolates tested inhibitory activities against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages. Compounds 21 24 showed potent NO with IC₅₀ values 0.006 0.076 µM, respectively.

The barks and roots of Edgeworthia chrysantha LINDL., which have been used as the folk medicine "Zhu shima" in southern China due to their detumescence acesodyne effects, were investigated for anti-inflammatory analgesic activities using a xylene-induced ear edema assay mice Freund's complete adjuvant-induced paw inflammation models, acetic acid-induced writhing test an model. Fractions effective terms obtained from E. chrysantha. chloroform-soluble fraction (CHF) showed significant...

Myelin and lymphocyte protein 2 (MAL2) is mainly involved in endocytosis under physiological conditions mediates the transport of materials across membranes cell organelle. It has been reported that MAL2 significantly upregulated diverse cancers. This study aimed to investigate role breast cancer (BC).Bioinformatics analysis Immunohistochemical assay were applied detect correlation between expression tissues prognosis patients. Functional experiments carried out vitro vivo. The molecular...

Long noncoding RNAs (lncRNAs) have been demonstrated to regulate reproduction in mammals. Our previous study revealed that the expression level of lncRNA-Gm2044 was obviously elevated nonobstructive azoospermia with spermatogonial arrest. Here, a transgenic mouse model spermatogonia using Stra8 promoter constructed explore roles upregulated male fertility. Testicular morphology and fertility weren't affected mice expressing lncRNA-Gm2044. However, overexpression partially impaired...

The diagnosis and treatment of unexplained recurrent spontaneous abortion (URSA) are subject to debate, because the exact underlying mechanisms remain unclear. To address this issue, we elucidated expression profiles dysregulated circRNAs, miRNAs, mRNAs constructed circRNA-associated competitive endogenous RNA (ceRNA) networks by comparing decidua URSA with that normal early pregnancy (NEP) using RNA-sequencing. In total, 550 mRNAs, 88 139 circRNAs were differentially expressed (DE) in URSA....

Eukaryotic translation initiation factor 4E (eIF4E) is a key regulator of protein synthesis. Changes in eIF4E activity disproportionally affect the subset oncogenic mRNAs some cancers.We have assessed expression levels vascular endothelial growth C (VEGFC), eIF4E, eIF4E-binding proteins (4E-BPs) and phospho-4E-BP1 clear cell renal carcinoma (ccRCC; n=101) using immunohistochemistry analyzed relevant mRNA survival online databases.The VEGFC, an eIF4E-regulated gene, were upregulated ccRCC...

Ubiquitin carboxyl terminal hydrolase L1 (UCH-L1) is one of the deubiquitinating enzymes in ubiquitin-proteasome system. It has been shown that UCH-L1 could markedly decrease neointima formation through suppressing vascular smooth muscle cell (VSMC) proliferation balloon-injured rat carotid. However, whether plays roles VSMC migration remains to be determined. In this study, primary VSMCs were isolated from aortic media rats and TNF-α was used induce migration. Using a modified Boyden...

To observe the changes of Prohibitin1(PHB1) contents in rat brain, heart, skeletal muscle tissue and mitochondria with acute exhaustive exercise effects on mitochondrial function rats, to explore relationship among PHB1 energy metabolism.Acute model:The rats carried after 8 weeks feeding. The brain samples were collected detect respiratory reactive oxygen species(ROS). expression protein tissues was detected by Western blot. ATP content organs activity complexes (ATP synthase activity)...

ADVERTISEMENT RETURN TO ISSUEPREVAddition/CorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to First Ruthenium(II) Polypyridyl Complex As True Molecular "Light Switch" for Triplex RNA Structure: [Ru(phen)2(mdpz)]2+ Enhances the Stability of Poly(U)·Poly(A)*Poly(U)Li-Feng Tan*, Jing Liu, Jian-Liang Shen, Xiao-hua Le-Li Zeng, and Lian-He JinCite this: Inorg. Chem. 2013, 52, 10, 6230Publication Date (Web):May 9, 2013Publication History Published online9 May 2013Published...