A5011712469- Estrogen and related hormone effects
- Genetics and Neurodevelopmental Disorders
- Reproductive System and Pregnancy
- Reproductive biology and impacts on aquatic species
- Developmental Biology and Gene Regulation
- Neurogenesis and neuroplasticity mechanisms
- Autism Spectrum Disorder Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ichthyology and Marine Biology
- Genomic variations and chromosomal abnormalities
- IL-33, ST2, and ILC Pathways
- Congenital heart defects research
- MicroRNA in disease regulation
- Subterranean biodiversity and taxonomy
- Zebrafish Biomedical Research Applications
- Pesticide Exposure and Toxicity
- Genomics and Rare Diseases
- Marine Ecology and Invasive Species
- Aquaculture Nutrition and Growth
- Microtubule and mitosis dynamics
- Carcinogens and Genotoxicity Assessment
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neuroendocrine regulation and behavior
- Effects and risks of endocrine disrupting chemicals
Centre National de la Recherche Scientifique
2013-2024
Immunologie et Neurogénétique Expérimentales et Moléculaires
2015-2024
Université d'Orléans
2013-2023
Centre de Biophysique Moléculaire
2016
University of Geneva
2016
Biomedical Research Institute
2016
Institut de Génomique Fonctionnelle
2016
Centre National pour la Recherche Scientifique et Technique (CNRST)
2015
Sorbonne Université
2010
Université Paris Cité
2010
There are two estrogen receptor (ER) subtypes in fish, ERα and ERβ, increasing evidence that the ERβ subtype has more than one form. However, there is little information on characteristics functional significance of these ERs adults during development. Here, we report cloning characterization three ER forms, zfERα, zfERβ1, zfERβ2, zebrafish. The percentages identity between receptors suggest existence distinct genes. Each cDNA encoded a protein specifically bound estradiol with dissociation...
Abstract Compared with other vertebrates, the brain of adult teleost fish exhibits two unique features: it unusually high neurogenic activity and strongly expresses aromatase, a key enzyme that converts aromatizable androgens into estrogens. Until now, these features, aromatase activities, have never been related to each other. Recently, was shown is expressed in radial glial cells forebrain not neurons. Here, we further document Aromatase B detected expressing markers postmitotic neurons,...
Compared with adult mammals, the brain of teleost fish is characterized by an extremely high capacity to aromatize androgens into estrogens, and this metabolic activity results from expression a specific aromatase (AroB) generated cyp19b gene. In study, we first antibodies zebrafish AroB used them map AroB-positive structures in zebrafish. We show that exclusively expressed radial glial cells, mainly olfactory bulbs, telencephalon, preoptic area, hypothalamus. Second, investigated vivo vitro...
Neuropathic pain from injury to the peripheral and CNS represents a major health care issue. We have investigated role of IL-33/IL-33 receptor (ST2) signaling in experimental models neuropathic mice. Chronic constriction (CCI) sciatic nerve induced IL-33 production spinal cord. IL-33/citrine reporter mice revealed that oligodendrocytes are main cells expressing within cord together with minor expression by neurons, microglia. astrocytes. CCI-induced mechanical hyperalgesia was reduced IL-33R...
Abstract Fragile X syndrome (FXS) is an inherited form of intellectual disability caused by the loss mRNA-binding fragile mental retardation protein (FMRP). FXS characterized neuronal hyperexcitability and behavioral defects, however mechanisms underlying these critical dysfunctions remain unclear. Here, using male Fmr1 knockout mouse model FXS, we identify abnormal extracellular potassium homeostasis, along with impaired channel Kir4.1 expression function in astrocytes. Further, reveal that...
Abstract Recent data indicate that estrogens locally produced in the brain by aromatization of androgens could be important for neurogenesis and repair. In this respect, fish are interesting because extremely high aromatase activity their brain. study, rainbow trout was cloned riboprobes were used to map distribution cells expressing corresponding mRNAs. A very strong hybridization signal detected pituitary bordering ventricles telencephalon ventral diencephalon, with highest expression...
Ventral midline Sonic Hedgehog (Shh) signalling is crucial for growth and patterning of the embryonic forebrain. Here, we report how enhanced Shh affects evolution telencephalic diencephalic neuronal in blind cavefish Astyanax mexicanus, a teleost fish closely related to zebrafish. A comparison between cave- surface-dwelling forms shows that display larger expression all anterior domains throughout development. This does not affect global forebrain regional patterning, but has several...
Endocrine-disrupting effects of phthalates are understood primarily from in utero exposures within the fetal rat testis. Nevertheless, their path action, dose-response character, and cellular target(s) testis not known.In this study we investigated di-(2-ethylhexyl) phthalate (DEHP), mono-(2-ethylhexyl) (MEHP), several metabolites on development organo-cultured testes fetus.We removed 14.5-day-old fetuses cultured them for 1-3 days with or without DEHP, MEHP, metabolites.DEHP (10(-5) M)...
Fragile X Syndrome (FXS) is the most common form of inherited intellectual disability and also associated with autism spectrum disorders. Previous studies implicated BKCa channels in neuropathogenesis FXS, but main question was whether pharmacological stimulation would be able to rescue FXS neurobehavioral phenotypes.We used a selective channel opener molecule (BMS-204352) address this issue Fmr1 KO mice, modeling pathophysiology. In vitro, acute BMS-204352 treatment (10 μM) restored...
Abstract The sighted surface‐dwelling (surface fish, SF) and the blind cave‐living (cavefish, CF) forms of Astyanax mexicanus offer a unique opportunity to study evolutionary changes in developmental mechanisms that lead retinal degeneration. Previous data have shown role increased midline Sonic Hedgehog (Shh) signalling cavefish eye degeneration (Yamamoto et al. [ 2004 ] Nature 431:844–847). Here, we compared major steps development SF CF between 14 hours 5 days development. We analyzed...
Cerebral malaria (CM) is associated with a high mortality rate and long-term neurocognitive impairment in survivors. The murine model of experimental cerebral (ECM) induced by Plasmodium berghei ANKA (PbA)-infection reproduces several these features. We reported recently increased levels IL-33 protein brain undergoing ECM the involvement IL-33/ST2 pathway development. Here we show that PbA-infection early short term spatial memory defects, prior to blood barrier (BBB) disruption, wild-type...
INTRODUCTIONDue to their large size and long generation times, chondrichthyans have been largely ignored by geneticists. However, key phylogenetic position makes them ideal subjects study the molecular bases of important morphological physiological innovations that characterize jawed vertebrates. Such analyses are crucial understanding origin complex genetic mechanisms unraveled in osteichthyans. The small spotted dogfish Scyliorhinus canicula, a representative largest order extant sharks,...
Reelin-expressing Cajal-Retzius (CR) cells are among the earliest generated in mammalian cerebral cortex and believed to be crucial for both development evolution of a laminated pattern pallial wall telencephalon. LIM-homeodomain (LIM-hd) transcription factors expressed during brain highly restricted combinatorial manner, they specify regional cellular identity. We have investigated expression LIM-hd members Lhx1/Lhx2/Lhx5/Lhx6/Lhx9 reelin-expressing cells, pallium, regions origin CR...
Delineating candidate genes at the chromosomal breakpoint regions in apparently balanced chromosome rearrangements (ABCR) has been shown to be more effective with emergence of next-generation sequencing (NGS) technologies. We employed a large-insert (7–11 kb) paired-end tag technology (DNA-PET) systematically analyze genome four patients harbouring cytogenetically defined ABCR neurodevelopmental symptoms, including developmental delay (DD) and speech disorders. characterized structural...
Left-right asymmetries in the epithalamic region of brain are widespread across vertebrates, but their magnitude and laterality varies among species. Whether these differences reflect independent origins forebrain or taxa-specific diversifications an ancient vertebrate feature remains unknown. Here we show that catshark Scyliorhinus canicula lampreys Petromyzon marinus Lampetra planeri exhibit conserved molecular between left right developing habenulae. Long-term pharmacological treatments...
Abstract Background Interleukin (IL)-33 is expressed in a healthy brain and plays pivotal role several neuropathologies, as protective or contributing to the development of cerebral diseases associated with cognitive impairments. However, IL-33 poorly understood, raising question its involvement immunoregulatory mechanisms. Methods We administered recombinant (rmIL-33) by intra-hippocampal injection C57BL/6 J (WT) IL-1αβ deficient mice. Chronic minocycline administration was performed...
Abstract The cytochrome P450 Aromatase is the key enzyme catalyzing conversion of androgens into estrogens. In zebrafish, brain aromatase encoded by cyp19b . Expression restricted to radial glial cells bordering forebrain ventricles and strongly stimulated estrogens during development. At promoter level, we have previously shown that an estrogen responsive element (ERE) required for induction Here, investigated role ERE flanking regions in control cell‐specific expression. First, show a 20...
Visual sensory impairments are common in Mental Deficiency (MD) and Autism Spectrum Disorder (ASD). These defects linked to cerebral dysfunction the visual cortical area characterized by deregulation of axon growth/guidance dendrite spine immaturity neurons. However, perception had not been addressed, although retina is part central nervous system with a embryonic origin. Therefore, we investigated retinal perception, first event vision, murine model MD autistic features. We document that...
Circulating microRNAs (miRNAs) have aroused a lot of interest as reliable blood diagnostic biomarkers Alzheimer’s disease (AD). Here, we investigated the panel expressed miRNAs in response to aggregated Aβ1–42 peptides infused hippocampus adult rats mimic events early onset non-familial AD disorder. led cognitive impairments associated with an astrogliosis and downregulation circulating miRNA-146a-5p, -29a-3p, -29c-3p, -125b-5p, and-191-5p. We established kinetics expression selected found...
In oviparous species, in addition to a full-length estrogen receptor alpha (ERα), another ERα isoform lacking the A domain and exhibiting ligand-independent transactivation function has been consistently reported. Although both isoforms are expressed liver, their respective sites of expression other potential target tissues unknown. contrast situation Xenopus chicken, two rainbow trout (Oncorhynchus mykiss) generated from classes transcripts with different 5′ untranslated sequences issued...
Glufosinate ammonium (GLA) is one of the most widely used herbicides in agriculture. As case for pesticides, potential adverse effects GLA have not been studied from perspective developmental neurotoxicity. Early pesticides exposure may weaken basic structure developing brain and cause permanent changes leading to a wide range lifelong on health and/or behavior. Here, we addressed impact by exposing female mice low dose during both pre- postnatal periods analyzed behavioral offspring infancy...
Accumulating evidence suggests that developmental exposure to environmental chemicals may modify the course of brain development, ultimately leading neuropsychiatric / neurodegenerative disorders later in life. In present study, we assessed impact one most frequently used pesticides both residential and agricultural applications - synthetic pyrethroid cypermethrin (CYP) on neurotoxicity (DNT). Female mice were perinatally exposed low doses CYP (5 20 mg/kg body weight) from gestation...
Neurogenesis, a process of generating functional neurons from neural precursors, occurs throughout life in restricted brain regions such as the subventricular zone (SVZ). During this process, newly generated migrate along rostral migratory stream to olfactory bulb replace granule cells and periglomerular neurons. This neuronal migration is pivotal not only for plasticity but also adapted based behaviors. Perturbation highly controlled system by exogenous chemicals has been associated with...