A5012979592- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer Genomics and Diagnostics
- Hepatocellular Carcinoma Treatment and Prognosis
- Pancreatic and Hepatic Oncology Research
- Epigenetics and DNA Methylation
- Gallbladder and Bile Duct Disorders
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Mathematical Biology Tumor Growth
- Hepatitis B Virus Studies
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Cancer Mechanisms and Therapy
- Peptidase Inhibition and Analysis
- Neuroendocrine Tumor Research Advances
- Viral-associated cancers and disorders
- Liver physiology and pathology
- Pediatric Hepatobiliary Diseases and Treatments
- Cancer-related molecular mechanisms research
- Liver Disease and Transplantation
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Nutrition, Genetics, and Disease
- Genomics and Chromatin Dynamics
- Genomics and Phylogenetic Studies
Keio University
2015-2024
Keio University Hospital
2018-2022
National Cancer Centre Japan
2005-2019
National Cancer Research Institute
2005-2018
Japan Agency for Medical Research and Development
2018
Tokyo Medical and Dental University
2017
Creative Research Enterprises (United States)
2017
Tokyo National Hospital
2008-2017
National Cancer Center Hospital East
2017
National Cancer Center
2007-2014
Cholangiocarcinoma is an intractable cancer, with limited therapeutic options, in which the molecular mechanisms underlying tumor development remain poorly understood. Identification of a novel driver oncogene and applying it to targeted therapies for molecularly defined cancers might lead improvements outcome patients. We performed massively parallel whole transcriptome sequencing eight specimens from cholangiocarcinoma patients without KRAS/BRAF/ROS1 alterations identified two fusion...
Abstract Purpose: Tumor-infiltrating lymphocytes represent the host immune response to cancer. CD4+CD25+FOXP3+ regulatory T cells (Tregs) suppress reaction. The aim of present study was investigate clinicopathologic significance and roles Tregs CD8+ during hepatocarcinogenesis. Experimental Design: We examined infiltration FOXP3+ in tumor stroma nontumorous liver parenchyma using 323 hepatic nodules including precursor lesions, early hepatocellular carcinoma (HCC), advanced HCC, along with...
Epidermal growth factor receptor (EGFR), vascular endothelial (VEGF), and human epidermal 2 (HER2) have been considered as potential therapeutic targets in cholangiocarcinoma, but no studies yet clarified the clinicopathological or prognostic significance of these molecules. Immunohistochemical expression molecules was assessed retrospectively 236 cases well associations between factors clinical outcome. The proportions positive for EGFR, VEGF, HER2 overexpression were 27.4, 53.8, 0.9%...
Immune cells constitute an important element of tumor tissue. Accumulating evidence indicates their clinicopathological significance in predicting prognosis and therapeutic efficacy. Nonetheless, the combinations immune forming microenvironment association with histological findings remain largely unknown. Moreover, it is unclear which or microenvironments are most prognostically significant. Here, we comprehensively analyzed its intratumor heterogeneity 919 regions 158 hepatocellular...
Intrahepatic cholangiocarcinoma and combined hepatocellular show varying degrees of biliary epithelial differentiation, which can be defined as liver cancer displaying phenotype (LCB). LCB is second in the incidence for cancers with without chronic hepatitis background more aggressive than carcinoma (HCC). To gain insight into its molecular alterations, we performed whole-genome sequencing analysis on 30 LCBs. Here show, genome-wide substitution patterns LCBs developed livers overlapped...
Biliary tract carcinomas (BTCs) are among the most aggressive malignancies and have a poor prognosis. Here, we successfully established organoid lines derived from intrahepatic cholangiocarcinoma, gallbladder cancer, neuroendocrine carcinoma of ampulla Vater. These organoids BTCs were cultured stably for >1 year closely recapitulated histopathology, gene expression, genetic alterations evident in primary tumors. Gene expression profiling revealed that SOX2 could be potential prognostic...
Combined hepatocellular and cholangiocarcinoma (cHCC-CC) is an uncommon subtype of primary liver cancer, the clinicopathological features which have rarely been reported in detail. The aim this study was to clarify characteristics cHCC-CC comparison with carcinoma (HCC) (CC).The 26 patients, who were surgically treated, reviewed by comparing them patients suffering from ordinary showed greater similarity HCC than CC regard male/female ratio, status hepatitis viral infection, serum...
Recently the authors proposed a new staging and grading system for primary biliary cirrhosis (PBC) that takes into account necroinflammatory activity histological heterogeneity. Herein is convenient version of this system. Scores fibrosis, bile duct loss, chronic cholestasis were combined staging: stage 1, total score 0; 2, 1–3; 3, 4–6; 4, 7–9. Cholangitis (CA) hepatitis (HA) graded as CA0–3, HA0–3, respectively. Analysis interobserver agreement was then conducted. Digital images 62 needle...
The outcome of patients with advanced hepatocellular carcinoma (HCC) has remained unsatisfactory. Patients HCC suffer from chronic hepatitis or liver cirrhosis, and their reserve function is often limited.To develop new therapeutic agents that act specifically on but interfere only minimally residual function, we searched for genes were upregulated in 20 cases [namely, discovery sets 1 (n = 10) 2 10)] comparison corresponding nontumorous a panel representing normal organs using high-density...
Cholangiocarcinoma (CC) is a highly malignant carcinoma. We attempted to clarify the prognostic significance of c-Met overexpression and its association with clinicopathological factors in patients CC. One hundred eleven intrahepatic CC (IHCC) 136 extrahepatic (EHCC) who had undergone curative surgery were divided immunohistologically into c-Methigh c-Metlow groups. Clinicopathological outcomes compared between epidermal growth factor receptor (EGFR) expression was also examined 10 cell...
The hallmark of most cancer cells is the metabolic shift from mitochondrial to glycolytic metabolism for adapting surrounding environment. Although epigenetic modification intimately linked cancer, molecular mechanism, by which factors regulate metabolism, poorly understood. Here, we show that lysine-specific demethylase-1 (LSD1, KDM1A) has an essential role in maintaining human hepatocellular carcinoma cells. Inhibition LSD1 reduced glucose uptake and activity, with a concurrent activation...
Immune cells and tumor vessels constitute important elements in tissue; however, their detailed relationship human tumors, including HCC, is still largely unknown. Consequently, we expanded our previous study on the immune microenvironment of HCC analyzed among microenvironment, inflammatory/angiostatic factor expression, angiogenic vessel findings, encapsulating clusters (VETC) macrotrabecular-massive (MTM) patterns.We classified into four distinct immunovascular subtypes...
Cholangiocarcinoma is an intractable cancer, with no effective therapy other than surgical resection. Elevated vascular endothelial growth factor (VEGF) and epidermal receptor (EGFR) expressions are associated the progression of cholangiocarcinoma. We therefore examined whether inhibition VEGFR EGFR could be a potential therapeutic target for Vandetanib (ZD6474, ZACTIMA), VEGFR-2/EGFR inhibitor, was evaluated. Four human cholangiocarcinoma cell lines were molecularly characterised...
Genetic alterations and deregulation of the miRNA biogenesis pathway components have been reported in human tumors. Tissue-specific deletion Dicer gene, which encodes an essential processing enzyme, promotes carcinogenesis animal models. These features indicate that aberrant are directly associated with cancer. For present study, we conducted quantitative RT-PCR 14 genes related to 47 paired samples primary hepatocellular carcinoma (HCC) matched non-cancerous liver. Expression seven (Dgcr8,...