A5041990073- Genetic diversity and population structure
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Phylogenetic Studies
- Cocoa and Sweet Potato Agronomy
- Peanut Plant Research Studies
- Aquaculture disease management and microbiota
- Coconut Research and Applications
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Genetic Mapping and Diversity in Plants and Animals
- Quantum Dots Synthesis And Properties
- Fish Ecology and Management Studies
- Genetic and phenotypic traits in livestock
- Agricultural pest management studies
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- Angiogenesis and VEGF in Cancer
- Insect symbiosis and bacterial influences
- Occupational Health and Performance
- Plant Toxicity and Pharmacological Properties
- Wheat and Barley Genetics and Pathology
Mississippi State University
2011-2025
Biocom
2020-2023
Currently, there is still a need to improve the contiguity of rainbow trout reference genome and use multiple genetic backgrounds that will represent diversity this species. The Arlee doubled haploid line was originated from domesticated hatchery strain originally collected northern California coast. Canu pipeline used generate de-novo assembly high coverage PacBio long-reads sequence data. further improved with Bionano optical maps Hi-C proximity ligation data 32 major scaffolds...
Various obstacles are encountered by mammalian spermatozoa during their journey through the female genital tract, and only few or none will reach site of fertilization. Currently, there limited technical approaches for non-invasive investigation migration after insemination. As knowledge surrounding sperm behavior throughout tract still remains elusive, recent development self-illuminating quantum dot nanoparticles may present a potential means real-time in vitro vivo monitoring...
The fertility performance of animals is still a mystery and the full comprehension mammalian gametes maturation early embryonic development remains to be elucidated. recent in nanotechnology offers new opportunity for real-time study reproductive cells their physiological environments. As first step toward that goal, we evaluated effectiveness fluorescent luminescent nanoparticle vitro ex vivo imaging porcine gametes. Freshly harvested boar sperm were labeled with red-shifted (655 nm)...
Significance A great challenge for humanity is feeding its growing population while minimizing ecosystem damage and climate change. Here, we uncover the global benefits arising from introduction of one wild species accession to peanut-breeding programs decades ago. This work emphasizes importance biodiversity crop improvement: peanut cultivars with genetics this provided improved food security reduced use fungicide sprays. However, study also highlights perilous consequences changes in legal...
Atlantic salmon (Salmo salar) in Northeastern US and Eastern Canada has high economic value for the sport fishing aquaculture industries. Large differences exist between genomes of European origin North American (N.A.) origin. Given genetic genomic 2 lineages, it is crucial to develop unique resources N.A. salmon. Here, we describe that recently developed research aquaculture. Firstly, a new single nucleotide polymorphism (SNP) database consisting 3.1 million putative SNPs was generated...
Mango (Mangifera spp.) is a major tropical fruit crop of global economic importance, but advanced genomic resources are needed to support its breeding, conservation, and sustainable cultivation. In this study, mango pangenome was constructed using high-quality, telomere-to-telomere genomes four cultivated (M. indica) accessions representing distinct genetic origins, one wild relative, M. odorata. Genome-wide analyses revealed significant reduction in heterozygosity among elite commercial...
Abstract Efforts in genome sequencing the Aspergillus genus have led to development of quality reference genomes for several important species including A. nidulans, fumigatus, and oryzae. However, less progress has been made flavus. As part effort USDA-ARS Annual Aflatoxin Workshop Fungal Genome Project, isolate NRRL3357 was sequenced resulted a scaffold-level released 2005. Our goal biologically driven, focusing on two areas: variation aflatoxin production drought stress exacerbating by...
Virginia-type peanut, Arachis hypogaea subsp. hypogaea, is the second largest market class of peanut cultivated in United States. It mainly used for large-seeded, in-shell products. Historically, cultivars were developed through long-term recurrent phenotypic selection and wild species introgression projects. Contemporary genomic technologies represent a unique opportunity to revolutionize traditional breeding pipeline. While there are tools available peanuts, none tailored specifically...
Muscadine grape (Vitis rotundifolia) is resistant to many of the pathogens that negatively impact production common (V. vinifera), including bacterial pathogen Xylella fastidiosa subsp. (Xfsf), which causes Pierce's Disease (PD). Previous studies in have indicated Xfsf delays host immune response with a complex O-chain antigen produced by wzy gene. cultivars range from tolerant completely Xfsf, but mechanism unknown.We assembled and annotated new, long-read genome assembly for 'Carlos',...
The genome of the Swanson doubled haploid (DH) YY male line rainbow trout was de novo assembled using Canu pipeline, high-coverage PacBio long-read sequence data, Bionano optical maps, and Hi-C proximity ligation resulting in 29 major scaffolds aligning with karyotype (2 N = 58). This assembly, totaling 2.3 Gb an N50 52.4 Mb, represents approximately 95% chromosome sequences only 109 gaps between scaffolds. Notably, corrections to previous errors assembly were made, including identification...
Labeo rohita (rohu) is a carp important to aquaculture in South Asia, with production volume close Atlantic salmon. While genetic improvements rohu are ongoing, the genomic methods commonly used other improvement programs have historically been precluded rohu, partially due lack of high-quality reference genome. Here we present de novo genome produced using combination next-generation sequencing technologies, resulting 946 Mb consisting 25 chromosomes and 2,844 unplaced scaffolds. Notably,...
Abstract Background Channel catfish and blue are the most important aquacultured species in USA. The do not readily intermate naturally but F 1 hybrids can be produced through artificial spawning. by mating channel female with male exhibit heterosis provide an ideal system to study reproductive isolation hybrid vigor. purpose of was generate high-quality chromosome level reference genome sequences determine their genomic similarities differences. Results We present for both catfish,...
Abstract The Hydrangea genus belongs to the Hydrangeaceae family, in Cornales order of flowering plants, which early diverged among Asterids, and includes several species that are commonly used ornamental plants. Of them, macrophylla is one most valuable nursery trade, yet few genomic resources available for this crop or closely related Asterid species. Two high-quality haplotype-resolved reference genomes hydrangea cultivars ‘Veitchii’ ‘Endless Summer’ [highest quality at 2.22 gigabase...
A bstract : Relaxin is known to promote vascular endothelial growth factor (VEGF) expression in reproductive tissue, and successful wound healing depends on good vascularization of sites, a process that relaxin may facilitate. Thus, the objective this study was evaluate efficacy development tissue at sites novel VEGF receptor 2‐ luc (VEGFR2‐ ) transgenic mouse model by monitoring rate VEGFR2‐ ‐mediated gene using bioluminescence real‐time imaging. To end, 12 FVB/N male mice were assigned...
Consumption of wild-type (toxic) endophyte-infected tall fescue (E+) by horses during late gestation is known to adversely affect pregnancy outcome; however, little the potential disruptive consequences E+ consumption mares critical phases placentation and fetal development in early pregnancy. The objective this study was evaluate detrimental effects feeding gestation. Mares (n = 12) paired stage (d 65 100) were assigned diets (six per diet) consisting endophyte-free (E−) or seed (50% E−...
Cotton leaf curl virus (CLCuV) causes devastating losses to fiber production in Central Asia. Viral spread across Asia the last decade is causing concern that will further before resistant varieties can be bred. Current development depends on screening each generation under disease pressure a country where endemic. We utilized quantitative trait loci (QTL) mapping four crosses with different sources of resistance identify single nucleotide polymorphism (SNP) markers associated allow without...
The genetic basis of general plant vigor is major interest to food producers, yet the trait recalcitrant mapping because number loci involved, their small effects, and linkage. Observations heterosis in many crops suggests that recessive, malfunctioning versions genes are a cause poor performance, we have little information on mutational spectrum underlying these disruptions. To address this question, generated long-read assembly tropical japonica rice ( Oryza sativa ) variety, Carolina...
As sequencing costs decrease and availability of high fidelity long-read increases, generating experiment specific de novo genome assemblies becomes feasible. In many crop species, obtaining the a hybrid or heterozygous individual is necessary for systems that do not tolerate inbreeding investigating important biological questions, such as vigor. However, most assembly methods have been used in plants result merged single sequence representation true biologically accurate either haplotype...
Cryopreservation is an excellent tool for sperm banking in livestock animals. Although its utilisation widespread species such as cattle, application pigs limited due to various factors associated with either cryopreservation techniques or male-to-male variations. Indeed, the existence of so-called “good freezer” (or GF) and “poor (PF) males has been well established swine yet, potential screen these characteristics still being investigated. Few studies have reported molecular differences...
Abstract Given the genetic and genomic differences between Atlantic salmon of European origin North American (N.A.) origin, it is crucial to develop unique resources for each lineage. Here we describe that recently developed research in N.A. salmon. Firstly, a new single nucleotide polymorphism (SNP) database consisting 3.1 million putative SNPs was generated using data from whole genome resequencing 80 individuals; Secondly, high density 50K SNP array enriched genic regions containing three...
Peanut smut, caused by Thecaphora frezzii Carranza & J.C. Lindq., is an emerging threat for the global peanut industry. The disease’s destructive potential can be exemplified pod incidence values as high 70% and yield losses reaching 30%. Because fungicides have shown moderate but highly variable levels of control levels, development deployment smut resistant cultivars are best strategies disease management. Screening smut-resistant germplasm requires years field trials currently only...
Abstract Labeo rohita (rohu) is a carp important to aquaculture in South Asia, with production volume close Atlantic salmon. While genetic improvements rohu are ongoing, the genomic methods commonly used other improvement programs have historically been precluded rohu, partially due lack of high quality reference genome. Here we present high-quality de novo genome produced using combination next-generation sequencing technologies, resulting 946 Mb consisting 25 chromosomes and 2,844 unplaced...
Background: Vascular endothelial growth factor (VEGF) is essential for normal vascular and development during wound repair.VEGF estrogen responsive capable of regulating its own receptor, receptor-2 (VEGFR-2).Several agricultural pesticides (e.g., methoxychlor) have estrogenic potential that can initiate inappropriate physiological responses in estrogenic-sensitive tissues following exposure vivo.Thus, the current study was designed to determine whether VEGFR-2-Luciferase (Luc) reporter...