A5011045979- Zebrafish Biomedical Research Applications
- MicroRNA in disease regulation
- RNA modifications and cancer
- Ferroptosis and cancer prognosis
- Cancer-related molecular mechanisms research
- Glioma Diagnosis and Treatment
- CRISPR and Genetic Engineering
- Molecular Sensors and Ion Detection
- X-ray Diffraction in Crystallography
- Neuroinflammation and Neurodegeneration Mechanisms
- Hepatitis C virus research
- Advanced biosensing and bioanalysis techniques
- Effects and risks of endocrine disrupting chemicals
- Epigenetics and DNA Methylation
- Nanoparticles: synthesis and applications
- Phosphodiesterase function and regulation
- Hippo pathway signaling and YAP/TAZ
- Crystallization and Solubility Studies
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Phytochemical compounds biological activities
- Pharmacological Receptor Mechanisms and Effects
- Pluripotent Stem Cells Research
- Virus-based gene therapy research
- Developmental Biology and Gene Regulation
Shantou University Medical College
2017-2025
Shantou University
2013-2025
Guangdong Medical College
2025
First Affiliated Hospital of Shantou University Medical College
2024
First People's Hospital of Chongqing
2023-2024
Jiangnan University
2024
Shanghai Academy of Agricultural Sciences
2024
Beijing Normal University
2012-2024
Southern Medical University
2024
Nanfang Hospital
2024
Invasion and metastasis of solid tumors are the major causes death in cancer patients. Cancer stem cells (CSCs) constitute a small fraction tumor cell population, but play critical role invasion metastasis. The xenograft immunodeficient mice is one commonly used vivo models to study cells. However, this model time-consuming labor intensive. Zebrafish (Danio rerio) their transparent embryos emerging as promising system for studies invasion. In study, we established by using zebrafish embryo...
The widespread accumulation of nanoplastics is a growing concern for the environmental and human health. However, studies on mechanisms nanoplastic-induced developmental toxicity are still limited. Here, we systematically investigated potential biological roles nanoplastic exposure in zebrafish during early stage. embryos were subjected to 100 nm polystyrene with different concentrations (0, 100, 200, 400 mg/L). results indicated that could decrease hatching survival rates embryos. In...
Malfunctioned gap junctional intercellular communication (GJIC) has been thought associated with malignant transformation of normal cells. However, the role GJIC-related proteins such as connexins in sustaining behavior cancer stem cells remains unclear. In this study, we obtained tumorspheres formed by glioma (GSCs) and adherent GSCs then examined their GJIC. All showed reduced GJIC, differentiated had more junction-like structures than GSCs. expressed very low level connexins, Cx43...
Epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) resistance is a major obstacle in the treatment of non-small cell lung cancer (NSCLC). Epigenetic alterations have been shown to be involved NSCLC oncogenesis; however, their function EGFR-TKI remains uncharacterized. Here, we found that an EHMT2 inhibitor, UNC0638, can significantly inhibit and induce apoptosis EGFR-TKI-resistant cells. Additionally, also expression enzymatic activity levels were elevated Moreover,...
Sliver nanoparticles (AgNPs) are widely used in industry, agriculture, and medicine, potentially resulting adverse effects on human health aquatic environments. Here, we investigated the developmental toxicity of zebrafish embryos with acute exposure to AgNPs. Our results demonstrated defects 4 hpf after different concentrations AgNPs for 72 h. In addition, RNA-seq profiling treatment. Further Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses showed that...
The base excision repair (BER) pathway is an important defense response to oxidative DNA damage. It known that exposures phthalate esters (PAEs), including Dibutyl (DBP), Mono-(2-ethylhexyl) (MEHP), and Di-(2-ethylhexyl) (DEHP), cause reactive oxygen species-induced damage stress. Here, we determined the mRNA levels of BER pathway-related genes (ogg1, nthl1, apex1, parp1, xrcc1, lig3, ung, pcna, polb, pold, fen1, lig1), pro-apoptotic gene (bax), apoptotic suppressor (bcl2) in different...
Abstract Motivation Many studies have shown that IDH mutation and 1p/19q co-deletion can serve as prognostic signatures of glioma. Although these genetic variations affect the expression one or more genes, value gene related to status is still unclear. Results We constructed an ensemble pair signature for risk evaluation survival prediction glioma based on prior knowledge status. First, we separately built two IDH-GPS 1p/19q-GPS elucidated they were useful transcriptome markers projecting...
Abstract N6-methyladenosine (m6A) RNA methylation is the predominant epigenetic modification for mRNAs that regulates various cancer-related pathways. However, prognostic significance of m6A regulators remains unclear in glioma. By integrating TCGA lower-grade glioma (LGG) and glioblastoma multiforme (GBM) gene expression data, we demonstrated both m6A-target genes were associated with prognosis activated Then, paired their target as m6A-related pairs (MGPs) using iPAGE algorithm, among...
Here we synthesize silica-coated NaYF4:Yb/Er nanocomposites with a photosensitizer hypericin covalently bound to silica shells (UCNPs@SiO2@hypericin) successfully, exhibiting precise size-control, good water dispersity and excellent biocompatibility. Under near-infrared light (NIR) irradiation, UCNPs convert NIR strong green which agrees well the absorbance peak of hypericin, triggers generate singlet oxygen effectively. The cell apoptosis studies by flow cytometry, fluorescence microscope...
Heparanase is an endo‐β‐ d ‐glucuronidase that cleaves heparan sulfate (HS) side chains of proteoglycans. Compelling evidence tie heparanase levels with all steps tumor formation including initiation, growth, metastasis and chemo‐resistance, likely involving augmentation signaling pathways gene transcription. In order to reveal the molecular mechanism(s) underlying protumorigenic properties heparanase, we established inducible (Tet‐on) system in U87 human glioma cells applied array...
Highlights•VEGF-A stimulation induces cGAS nuclear translocation via importin-β pathway•Nuclear modulates VEGF-A-mediated angiogenesis regulating miR-212-5p-ARPC3 cascade•cGAS loss impairs trans-Golgi network-involved VEGFR2 trafficking•Nuclear of is associated with human pathologySummaryCyclic GMP-AMP synthase (cGAS) senses cytosolic incoming DNA and consequently activates stimulator interferon response cGAMP interactor 1 (STING) to mount immune response. Here, we show could regulate in an...
Phosphodiesterase type 5 (PDE5) inhibitors are first-line therapy for pulmonary arterial hypertension (PAH) and erectile dysfunction. As a continuing work to improve the terminal half-lives oral bioavailabilities of our previously reported 4(3H)-pyrimidones, pharmacokinetics-driven optimization focusing on substituent is described. Two major congeneric series aminosulfonylphenylpyrimidones acylaminophenylpyrimidones, were designed, synthesized, pharmacologically assessed in vitro vivo. Among...
Introduction: Silver nanoparticles (AgNP) are widely used as coating materials. However, the potential risks of AgNP to human health, especially for neural and vascular systems, still poorly understood. Methods: The neurotoxicity various concentrations in zebrafish were examined using fluorescence microscopy. In addition, Illumina high-throughput global transcriptome analysis was performed explore profiles embryos after exposure AgNP. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment...
Pancreatic cancer remains one of the leading causes mortality worldwide, largely due to limitations current clinical strategies for its treatment. As a result, identifying genetic alterations and potential therapeutic targets could offer new opportunities improving diagnosis treatment pancreatic cancer. The identification differentially expressed genes (DEGs) subsequent analyses, including signaling pathway enrichment, functional classification, protein-protein interaction (PPI) network...
Cyclic nucleotide phosphodiesterase type 5 (PDE5) is a prime drug target for treating the diseases associated with lower level of cyclic guanosine monophosphate (cGMP), which specific substrate PDE5 hydrolysis. Here we report series novel inhibitors new scaffold monocyclic pyrimidin-4(3H)-one ring developed using structure-based discovery strategy. In total, 37 derivatives pyrimidin-4(3H)-ones, were designed, synthesized, and evaluated their inhibitory activities to PDE5, resulting in 25...
Erythropoietin (EPO) is an attractive protein-unfolding/folding model because of its high degree unfolding and folding reversibility intermediate size. Due to function for regulating red blood cell production by stimulating late erythroid precursor cells, EPO presents obvious values biological research. A nonemissive anthracene derivative, that 9,10-bis[4-(3-sulfonatopropoxyl)-styryl]anthracene sodium salt (BSPSA), with aggregation-induced emission (AIE) charateristics shows a novel...