A5034525099- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Immunotherapy and Immune Responses
- interferon and immune responses
- Influenza Virus Research Studies
- Gut microbiota and health
- Virology and Viral Diseases
- vaccines and immunoinformatics approaches
- RNA Research and Splicing
- Immune Cell Function and Interaction
- RNA regulation and disease
- Whipple's Disease and Interleukins
- RNA and protein synthesis mechanisms
- Long-Term Effects of COVID-19
- Immune Response and Inflammation
- Diet and metabolism studies
- SARS-CoV-2 detection and testing
- Inflammasome and immune disorders
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Institute of Medical Biology
2014-2024
Peking Union Medical College Hospital
2014-2023
Kunming University
2014
Academy of Medicine
2014
Institut de Recherche Vaccinale
2014
Enterovirus 71 (EV71) is a major cause of hand, foot, and mouth disease in children may be fatal. A vaccine against EV71 needed.We conducted randomized, double-blind, placebo-controlled phase 3 trial involving healthy 6 to months age Guangxi Zhuang Autonomous Region, China. Two doses an inactivated or placebo were administered intramuscularly, with 4-week interval between doses, monitored for up 11 months. The primary end point was protection caused by EV71.A total 12,000 randomly assigned...
While lymphocytopenia is a common characteristic of coronavirus disease 2019 (COVID-19), the mechanisms responsible for this lymphocyte depletion are unclear. Here, we retrospectively reviewed clinical and immunological data from 18 fatal COVID-19 cases, results showed that these patients had severe lymphocytopenia, together with high serum levels inflammatory cytokines (IL-6, IL-8 IL-10), elevation many other mediators in routine laboratory tests, including C-reactive protein, lactate...
The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. novel combination of DNA vaccine encoding the full-length Spike (S) protein SARS-CoV-2 recombinant S1 induced high level neutralizing antibody T cell immune responses in both small large animal models. More significantly, co-delivery components at same time elicited full protection against intratracheal challenge viruses immunized rhesus macaques. As vaccines have been proven previous human studies, are capable...
Coronavirus disease 2019, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), leads to a series of clinical symptoms and pulmonary inflammatory reactions via unknown pathologic mechanisms related the viral infection process in tracheal or bronchial epithelial cells. Investigation this human cell line (16HBE) suggests that SARS-CoV-2 can enter these cells through interaction between its membrane-localized S protein with angiotensin-converting enzyme 2 molecule on host...
Enterovirus 71 (EV71), a major causative agent of hand-foot-and-mouth disease (HFMD), causes outbreaks among children in the Asia-Pacific region. A vaccine is urgently needed. Based on successful pre-clinical work, phase I and II clinical trials an inactivated EV71 vaccine, which included participants 288 660 respectively, have been conducted. In present study, immune response correlated modulation gene expression peripheral blood mononuclear cells (PBMCs) 30 infants (6 to 11 months)...
UL7, a tegument protein of Herpes Simplex Virus type I (HSV-1), is highly conserved in viral infection and proliferation has an unknown mechanism action. A HSV-1 UL7 mutant (UL7-MU) was constructed using the CRISPR-cas9 system. The replication rate plaque morphology were used to analyze biological characteristics wild-type (WT), UL7-MU MU-complemented P1 viruses. virulence viruses evaluated mice. Real-time RT-qPCR ChIP assays determine expression levels relevant genes. capacity recombinant...
SARS-CoV-2 caused the COVID-19 pandemic that lasted for more than a year. Globally, there is an urgent need to use safe and effective vaccines immunization achieve comprehensive protection against infection. Focusing on developing rapid vaccine platform with significant immunogenicity as well broad high efficiency, we designed spike protein receptor-binding domain (RBD) displayed self-assembled ferritin nanoparticles. In 293i cells eukaryotic expression system, this candidate was prepared...
An ideal vaccine against SARS-CoV-2 is expected to elicit broad immunity prevent viral infection and disease, with efficient clearance in the upper respiratory tract (URT). Here, N protein prefusion-full S (SFLmut) are combined flagellin (KF) cyclic GMP-AMP (cGAMP) generate a candidate vaccine, this elicits stronger systemic mucosal humoral than vaccines containing other forms of protein. Furthermore, administered via intranasal route can enhance local immune responses tract. Importantly,...
To investigate the long-term effects on immunity of an inactivated enterovirus 71 (EV71) vaccine and its protective efficacy. A sub-cohort 1,100 volunteers from Guangxi Province in China was eligible for enrolment randomly administered either EV71 or a placebo days 0 28 phase III clinical trial then observed following 2 years with approval by independent ethics committee Zhuang Autonomous Region, China. Serum samples 350 participants who provided full series blood (at all sampling points)...
The pathological manifestations of fatal cases human hand, foot and mouth disease (HFMD) caused by enterovirus 71 (EV71) are characterized inflammatory damage to the central nervous system (CNS). Here, dynamic distribution EV71 in CNS subsequent characteristics within different regions neonatal rhesus macaque brain tissue were studied using a chimeric expressing green fluorescence protein. results compared with obtained from autopsies deceased EV71-infected HFMD patients. These observations...
Respirovirus such as influenza virus infection induces pulmonary anti-viral immune response, orchestration of innate and adaptive immunity restrain viral infection, otherwise causes severe diseases pneumonia. Chemokines regulate leukocyte recruitment to the inflammation site. One chemokine CXCL5, plays a scavenging role host defense against bacterial but its in is underdetermined. Here, using an (H1N1) infected CXCL5 -/- mouse model, we found that not only responds neutrophil infiltration...
Neutralizing antibodies in the subjects of an inactivated SARS-CoV-2 vaccine clinical trial showed a decreasing trend over months. An investigation studying third immunization suggested that waning neutralizing individuals administered two doses does not mean disappearance immunity.
Herpes simplex virus type 1 (HSV-1) is a very concerning pathogen due to its ability persist in the host’s nervous system and continuously interfere with immune system, which complicates treatment. Therefore, development of an effective HSV-1 vaccine crucial. In this study, we focused on mutant strain, M6, includes several deleted genes associated viral infection virulence latent function, explored macrophages immunological characteristics. The study found that both attenuated strain M6...
The coxsackie A16 virus (CA16), along with enterovirus 71 (EV71), is a primary pathogen that causes hand, foot, and mouth disease (HFMD). To control HFMD, CA16, EV71 vaccines are needed. In this study, an experimental inactivated CA16 vaccine was prepared using human diploid cells, the vaccine's immunogenicity analyzed in mice rhesus monkeys. results showed neutralizing antibody developed dose-dependent manner, sustained for 70 days average GMT (geometric mean titer) level of 80 to 90...
Enterovirus 71 (EV-A71) and coxsackievirus A16 (CV-A16) remain the predominant etiological agents of hand, foot, mouth disease (HFMD). The clinical manifestations caused by two viruses are obviously different. CV-A16 usually triggers a repeated infection, airway epithelial integrity is often potential causative factor respiratory infections. Our previous studies have demonstrated that there were some differentially expressed miRNAs involved in regulation adhesion function barrier EV-A71 In...
Platelets have been implicated in pulmonary inflammation following exposure to bacterial stimuli. The mechanisms involved the platelet-mediated host response respiratory infection remain incompletely understood. In this study, we demonstrate that platelet-derived chemokine (C-X-C motif) ligand 4 (CXCL4) plays critical roles a mouse model of acute pneumonia using Pseudomonas aeruginosa. are activated during P. aeruginosa infection, and mice depleted platelets display markedly increased...