A5026257121- Advanced Chemical Sensor Technologies
- Gut microbiota and health
- Infant Nutrition and Health
- Neonatal and Maternal Infections
- Neonatal Respiratory Health Research
- Essential Oils and Antimicrobial Activity
- Streptococcal Infections and Treatments
- Biosensors and Analytical Detection
- Neonatal and fetal brain pathology
- Infant Health and Development
- Asthma and respiratory diseases
- Neuroscience of respiration and sleep
- Probiotics and Fermented Foods
- Cystic Fibrosis Research Advances
- Mycobacterium research and diagnosis
- Diphtheria, Corynebacterium, and Tetanus
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
- Clinical Nutrition and Gastroenterology
- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Inflammatory Bowel Disease
- Gastrointestinal motility and disorders
- Sulfur Compounds in Biology
- Fatty Acid Research and Health
Emma Kinderziekenhuis
2018-2025
Amsterdam University Medical Centers
2018-2025
University of Amsterdam
2018-2024
Vrije Universiteit Amsterdam
2018-2021
Amsterdam UMC Location University of Amsterdam
2018-2020
Amsterdam UMC Location Vrije Universiteit Amsterdam
2018-2020
Robert Bosch (India)
2020
<b><i>Background:</i></b> Late-onset sepsis (LOS) in preterm infants is a leading cause of mortality and morbidity. Timely recognition initiation antibiotics are important factors for improved outcomes. Identification risk could allow selection at an increased LOS. <b><i>Objectives:</i></b> The aim was to identify <b><i>Methods:</i></b> In this multicenter case-control study, born ≤30 weeks gestation were included 9...
Abstract Background The role of intestinal microbiota in the pathogenesis late-onset sepsis (LOS) preterm infants is largely unexplored but could provide opportunities for microbiota-targeted preventive and therapeutic strategies. We hypothesized that composition changes before onset sepsis, with causative bacteria are isolated later blood culture. Methods This multicenter case-control study included born under 30 weeks gestation. Fecal samples collected from 5 days preceding LOS diagnosis...
Early detection of late-onset sepsis (LOS) in preterm infants is crucial since timely treatment initiation a key prognostic factor. We hypothesized that fecal volatile organic compounds (VOCs), reflecting microbiota composition and function, could serve as non-invasive biomarker for preclinical pathogen-specific LOS detection. Fecal samples clinical data all (≤30 weeks' gestation) admitted at nine neonatal intensive care units the Netherlands Belgium were collected daily. Samples from one to...
Fecal volatile organic compounds (VOCs) are increasingly considered to be potential noninvasive, diagnostic biomarkers for various gastrointestinal diseases. Knowledge of the influence sampling conditions on VOC outcomes is limited. We aimed evaluate effects fecal profiles and assess under which an optimal accuracy in discrimination between pediatric inflammatory bowel disease (IBD) controls could obtained. samples from de novo treatment-naïve IBD patients healthy (HC) were used compared...
Abstract Necrotizing enterocolitis (NEC) is one of the most common and lethal gastrointestinal diseases in preterm infants. Early recognition infants need for surgical intervention might enable early intervention. In this multicenter case-control study, performed nine neonatal intensive care units, born (< 30 weeks gestation) diagnosed with NEC (stage ≥ IIA) between October 2014 August 2017 were divided into two groups: (1) medical (conservative treatment) (2) (sNEC). Perinatal, clinical,...
Alterations in fecal microbiota and volatile organic compound (VOC) profiles of preterm infants have been demonstrated before onset necrotizing enterocolitis (NEC). However, NEC-specific signatures need to be identified potential application as predictive biomarker clinical practice. A prospective multicenter case–control study was conducted identify preclinical VOC that developed NEC. Microbiota analysis (PCR-based IS-pro technique) (gas chromatography-mass spectrometry) were performed on...
Abstract Introduction Headspace gas chromatography–mass spectrometry (HS-GC–MS) is widely considered the gold standard of quantitative fecal VOC analysis. However, guidelines providing general recommendations for bioanalytical method application in research and clinical setting are lacking. Objectives To propose an evidence-based protocol analysis by HS-GC–MS, based on extensive testing instrumental sampling conditions detection quantification limits, linearity, accuracy repeatability...
Increasing interest is noticed in the potential of volatile organic compound (VOC) analysis as non-invasive diagnostic biomarker clinical medical practice. The spectrum VOCs, originating from (patho)physiological metabolic processes human body and detectable bodily excrements, such exhaled breath, urine feces, harbors a magnificent source information. Thus far, majority studies have focused on VOC aiming at identification disease-specific profiles. Recently, an increasing number evaluated...
Endoscopic evaluation is mandatory in establishing the diagnosis of pediatric inflammatory bowel disease (IBD), but unfortunately carries a high burden on patients. Volatile organic compounds (VOC) have been proposed as alternative, noninvasive diagnostic biomarkers for IBD. The current study aimed to assess and compare potential fecal urinary VOC IBD an intention-to-diagnose cohort. In this cohort study, patients aged 4-17 years, referred outpatient clinic tertiary referral center under...
Fecal volatile organic compound (VOC) analysis has shown great potential as a noninvasive diagnostic biomarker for variety of diseases. Before clinical implementation, the factors influencing outcome VOC need to be assessed. Recent studies found that sampling conditions can influence analysis. However, dietary influences remains unknown, especially in (preterm) infants. Therefore, we assessed effects feeding composition on fecal patterns preterm infants (born at <30 weeks gestation). Two...
Emerging evidence exists that an altered gut microbiota is a key factor in the pathophysiology of variety diseases. Consequently, microbiota-targeted interventions, including administration probiotics, have increasingly been evaluated. Mechanisms on how probiotics contribute to homeostasis or reverse (effects of) dysbiosis remain yet be elucidated. In current study, we assessed effects daily Lactobacillus casei strain Shirota (LcS) ingestion healthy children aged from 12-18 years...
Fecal volatile organic compounds (VOC) reflect human and gut microbiota metabolic pathways their interaction. VOC behold potential as non-invasive preclinical diagnostic biomarkers in various diseases, e.g., necrotizing enterocolitis late onset sepsis. There is a need for standardization assessment of the influence clinical environmental factors on outcome before this technique can be applied practice. The aim study was to investigate gestational age (GA) mode delivery fecal pattern preterm...
Abstract Background The fecal microbiota and metabolome are hypothesized to be altered before late-onset neonatal meningitis (LOM), analogous sepsis (LOS). present study aimed identify composition volatile metabolomics preceding LOM. Methods Cases gestational age-matched controls were selected from a prospective, longitudinal preterm cohort (born &lt;30 weeks’ gestation) at 9 intensive care units. microbial (16S rRNA sequencing) (gas chromatography-ion mobility spectrometry [GC-IMS]...
Infants developing necrotizing enterocolitis (NEC) have a different metabolomic profile compared to controls. The potential of specific metabolomics, i.e. amino acids and alcohols (AAA), as early diagnostic biomarkers for NEC is largely unexplored. In this multicenter prospective case-control study, longitudinally collected fecal samples from preterm infants (born <30 weeks gestation) 1-3 days before diagnosis severe (Bell's stage IIIA/IIIB), were analyzed by targeted high-performance liquid...
ABSTRACT In this prospective intention‐to‐diagnose pilot study, we aimed to assess accuracy of serum and fecal amino‐acids discriminate de novo pediatric inflammatory bowel disease (IBD) non‐IBD children. Patients with suspected IBD were allocated the (n = 11) or group 8) following laboratory testing endoscopy according revised Porto‐criteria. Fecal calprotectin levels obtained, an additional blood sample collected. amino‐acid profiles analyzed using high performance‐liquid chromatography....
Accurate prediction of preterm birth is currently challenging, resulting in unnecessary maternal hospital admittance and fetal overexposure to antenatal corticosteroids. Novel biomarkers like volatile organic compounds (VOCs) hold potential for predictive, bed-side clinical applicability. In a proof principle study, we aimed assess the predictive urinary identification pregnant women at risk birth. Urine samples with high (≧24 + 0 until 36 6 weeks) were collected prospectively analyzed VOCs...
Bloodstream infections (BSIs) cause treatment-related mortality in pediatric acute leukemia. We explored the potential of intestinal microbiota and fecal volatile organic compounds (VOCs) analyses to predict BSI.In this case-control study, samples leukemia patients were collected. Microbiota composition VOC profiles BSI cases matched non-BSI controls compared.In total, 6 included, which 1 developed neutropenic fever. Both showed reduced microbial diversity stability Bacteroidetes. In case,...
Objective: Prediction of preterm birth is currently not feasible, resulting in maternal and fetal overexposure to prenatal corticosteroids unnecessary hospital admittance. Novel biomarkers seem hold potential for predictive applicability, including non-invasive volatile organic compounds. In this study, we aimed assess the urinary compound profiles (VOCs) identification pregnant women at risk birth. Design, setting, population: We prospectively collected urine admitted imminent (≧ 24+0 weeks...