A5020833067- Microbial Natural Products and Biosynthesis
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Fungal and yeast genetics research
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Plant tissue culture and regeneration
- Entomopathogenic Microorganisms in Pest Control
- Cancer Research and Treatments
- Mycotoxins in Agriculture and Food
- Fungal Infections and Studies
- Nematode management and characterization studies
- Signaling Pathways in Disease
- Fungal Biology and Applications
- Genetic Mapping and Diversity in Plants and Animals
- Occupational and environmental lung diseases
- RNA and protein synthesis mechanisms
- Cancer Genomics and Diagnostics
- Insect-Plant Interactions and Control
- Peptidase Inhibition and Analysis
- Fermentation and Sensory Analysis
Duke Medical Center
2024-2025
Hainan Medical University
2025
University of Chicago
2025
Wuhan University
2025
Kansas State University
2019-2024
Fujian Agriculture and Forestry University
2016-2024
Sichuan University
2018-2024
Duke University Hospital
2024
Hangzhou DAC Biotech (China)
2019-2024
Revolution Medicines (United States)
2021-2023
SUMMARY In eukaryotes, alternative splicing ( AS ) greatly expands the diversity of transcripts. However, it is challenging to accurately determine full‐length isoforms. Recently, more studies have taken advantage Pacific Bioscience (PacBio) long‐read sequencing identify Nevertheless, high error rate PacBio reads seriously offsets advantages long reads, especially for identifying junctions. To best capitalize on features we used Illumina RNA ‐seq improve circular consensus sequence CCS...
Transcriptional dynamic in response to environmental and developmental cues are fundamental biology, yet many mechanistic aspects poorly understood. One such example is fungal plant pathogens, which use secreted proteins small molecules, termed effectors, suppress host immunity promote colonization. Effectors highly expressed planta but remain transcriptionally repressed ex , our understanding of these transcriptional dynamics remains limited. We tested the hypothesis that repressive histone...
Abstract Background RNA-targeting CRISPR-Cas can provide potential advantages over DNA editing, such as avoiding pleiotropic effects of genome providing precise spatiotemporal regulation, and expanded function including antiviral immunity. Results Here, we report the use CRISPR-Cas13 in plants to reduce both viral endogenous RNA. Unexpectedly, observe that crRNA designed guide Cas13 could, absence protein, cause substantial reduction RNA levels well. We demonstrate Cas13-independent...
Podoplanin (Aggrus), which is a type I transmembrane sialomucin-like glycoprotein, highly expressed in malignant pleural mesothelioma (MPM). We previously reported the generation of rat anti-human podoplanin Ab, NZ-1, inhibited podoplanin-induced platelet aggregation and hematogenous metastasis. In this study, we examined antitumor effector functions NZ-1 NZ-8, novel rat-human chimeric Ab generated from including Ab-dependent cellular cytotoxicity (ADCC) complement-dependent against MPM...
One major threat to global food security that requires immediate attention, is the increasing incidence of host shift and expansion in growing number pathogenic fungi emergence new pathogens. The more alarming because, yield quality quantity improvement efforts are encouraging cultivation uniform plants with low genetic diversity increasingly susceptible emerging However, influence genome differentiation on pathogen its contribution adaptability still obscure. Here, we compared sequence 6...
Brain size and cognitive skills are the most dramatically changed traits in humans during evolution yet genetic mechanisms underlying these human-specific changes remain elusive. Here, we successfully generated 11 transgenic rhesus monkeys (8 first-generation 3 second-generation) carrying human copies of
Abstract CRISPR-Cas mediated genome engineering has revolutionized functional genomics. However, understanding of DNA repair following Cas-mediated cleavage remains incomplete. Using Cas12a ribonucleoprotein editing in the fungal pathogen, Magnaporthe oryzae , we detail non-canonical outcomes from hundreds transformants. Sanger and nanopore sequencing analysis reveals significant variation profiles, ranging small INDELs to kilobase size deletions insertions. Furthermore, find frequency...
Histone chaperones (HCs) are crucial for governing genome stability and gene expression in multiple cancers. However, the functioning of HCs tumor microenvironment (TME) is still not clearly understood.Self-tested single-cell RNA-seq data derived from 6 breast cancer (BC) patients with brain liver metastases were reanalyzed by nonnegative matrix factorization (NMF) algorithm 36 HCs. TME subclusters observed BC immunotherapy public cohorts to assess their prognosis immune response. The...
Abstract Preimplantation genetic screening (PGS) detects chromosomal aneuploidy from DNA extracted trophectodermal biopsy of the embryos before implantation. Although a controlled study showed no difference in pregnancy rates between this invasive cell technique and non-biopsied control group, potential long-term damage by current PGS method has not be completely ruled out. We therefore tested less-invasive protocol which utilizes spent culture medium combining with blastocoel fluid (ECB) to...
While increased mutation rates typically have negative consequences in multicellular organisms, hypermutation can be advantageous for microbes adapting to the environment. Previously, we identified two hypermutator
ABSTRACT Avermectin (AVM) and ivermectin (IVM) are potent pesticides acaricides which have been widely used during the past 30 years. As insect resistance to AVM IVM is greatly increasing, alternatives urgently needed. Here, we report two novel derivatives, tenvermectin A (TVM A) TVM B, considered a potential new generation of agricultural veterinary drugs. The molecules TVMs were designed based on structure pharmacological property comparisons among AVM, IVM, milbemycin (MBM). To produce...
Circular RNAs (circRNAs) regulate complex functional processes and play crucial roles in cancer development progression. It was reported that circKIF4 regulates the progression of triple-negative breast (TNBC). This study evaluates role distant metastasis metabolic reprogramming. RT-qPCR performed to verify expression circKIF4A cancer, liver metastatic tissues, cell lines. The function evaluated both vitro vivo through a series experiments, including migration glucose intake experiments....
Abstract Several observations indicate that compatible ends of separate, yet closely linked, transposable elements (TEs) can interact in alternative transposition reactions. First, pairs TEs cause chromosome breaks with frequencies inversely related to the intertransposon distance. Second, some combinations two produce complex rearrangements often include DNA adjacent one or both elements. In direct orientation, reactions involving external should lead a macrotransposon consisting plus...
The yield of maize grain is a highly complex quantitative trait that controlled by multiple loci (QTLs) with small effects, and frequently influenced genetic environmental factors. Thus, it challenging to clone QTL for in the genome. Previously, we identified major QTL, qKNPR6, kernel number per row (KNPR) across environments, developed two nearly isogenic lines, SL57-6 Ye478, which differ only allelic constitution at short segment harboring QTL. Recently, qKNPR6 was re-evaluated segregating...
Alternative splicing (AS) is an important regulatory mechanism that greatly contributes to eukaryotic transcriptome diversity. A substantial amount of evidence has demonstrated AS complexity relevant evolution, development, adaptation, and complexity. In this study, six teosinte ten maize transcriptomes were sequenced analyze changes signatures selection in domestication improvement.In teosinte, 13,593 highly conserved genes, including 12,030 multiexonic detected. By identifying isoforms...
Abstract KRASG12V mutant cancers represent a significant unmet medical need with nearly 44,000 new diagnoses annually in the US. The mutation occurs frequently multiple tumor histotypes; incidence NSCLC, CRC and pancreatic is 6%, 10% 26%, respectively. RAS proteins are small GTPases that drive cell proliferation survival when bound to GTP. Mutant exist predominantly GTP-bound (RAS(ON)) state, leading excessive downstream signaling via interaction effectors such as RAF. intrinsic GTP...
Uncovering key genes that drive diseases and cancers is crucial for advancing understanding developing targeted therapies. Traditional differential expression analysis often relies on arbitrary cutoffs, missing critical with subtle changes. Some methods incorporate protein-protein interactions (PPIs) but depend prior disease knowledge. To address these challenges, we developed DiCE (Differential Centrality-Ensemble), a novel approach combines network centrality analysis, independent of...
To investigate the repellent efficacy of commercially available mosquito repellents in China against H. longicornis, four representative DEET-based and biont-derived were selected. The study utilized a modified circular filter paper method testing device to establish an evaluation system for assessing effects each product three developmental stages longicornis: larvae, nymphs, adults. In our study, repellents, Johnson demonstrated highest repellency larvae within 1 hour, with average rate...