A5055965377- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Helicobacter pylori-related gastroenterology studies
- MicroRNA in disease regulation
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Glioma Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Cancer-related Molecular Pathways
- Endoplasmic Reticulum Stress and Disease
- Drug Transport and Resistance Mechanisms
- PI3K/AKT/mTOR signaling in cancer
- Peptidase Inhibition and Analysis
- Metal complexes synthesis and properties
- Ferrocene Chemistry and Applications
- Histone Deacetylase Inhibitors Research
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Cytokine Signaling Pathways and Interactions
- Chemical Synthesis and Analysis
- Heat shock proteins research
- Nutrition and Health in Aging
- Frailty in Older Adults
- Epigenetics and DNA Methylation
Biogipuzkoa Health Research Institute
2015-2024
Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable
2018-2024
Instituto de Salud Carlos III
2022
Centro de Investigación Biomédica en Red
2020
Universitat de Miguel Hernández d'Elx
2004-2018
Fondazione IRCCS Istituto Nazionale dei Tumori
2012
Bayer (Germany)
2012
University of Milan
2012
Hospital General Universitario de Elche
2011
Instituto de Biologia Molecular e Celular
2005-2007
Background: SOX2 and SOX9 are commonly overexpressed in glioblastoma, regulate the activity of glioma stem cells (GSCs). Their specific overlapping roles GSCs treatment remain unclear.Methods: levels were examined human biopsies. Gain loss function determined impact altering on cell proliferation, senescence, activity, tumorigenesis chemoresistance.Results: expression correlates positively glioblastoma High bypass cellular senescence promote resistance to temozolomide. Mechanistic...
Abstract Gastric cancer remains one of the leading causes global mortality due to therapy resistance, with Helicobacter pylori (H. pylori) infection being a major risk factor. In this study, we report significance an elevation stem cell regulator SOX9 in bacteria-infected human gastritis and samples, paralleling increased levels TNFα. was more intense specimens containing pathogenically significant cagA+ strains H. pylori. Notably, found that required for bacteria-induced gastric...
Glioblastoma multiforme is the most frequent, aggressive and fatal type of brain tumor. Glioblastomas are characterized by their infiltrating nature, high proliferation rate resistance to chemotherapy radiation. Recently, oncologic therapy experienced a rapid evolution towards "targeted therapy," which employment drugs directed against particular targets that play essential roles in proliferation, survival invasiveness cancer cells. A number molecules involved signal transduction pathways...
Resistance of melanoma to targeted therapy and immunotherapy is linked metabolic rewiring. Here, we show that increased fatty acid oxidation (FAO) during prolonged BRAF inhibitor (BRAFi) treatment contributes acquired resistance in mice. Targeting FAO using the US Food Drug Administration-approved European Medicines Agency-approved anti-anginal drug ranolazine (RANO) delays tumour recurrence with BRAFi resistance. Single-cell RNA-sequencing analysis reveals RANO diminishes abundance...
Abstract Histone deacetylase 6 (HDAC6) is an epigenetic modifier that attractive pharmacological target in cancer. In this work, we show HDAC6 elevated glioblastoma, the most malignant and common brain tumor adults, which its high levels correlate with poor patient survival more abundant glioma stem cell subpopulation. Moreover, identified a new small-molecule inhibitor of HDAC6, presents strong sensitivity for inhibition exerts cytotoxic activity, alone or combination temozolomide. It also...
Abstract The developmental regulator SOX9 is linked to cancer progression mainly as a result of its role in the regulation stem cells (CSCs). However, activity differentiated that constitute heterogeneous tumor bulk has not been extensively studied. In this work, we addressed aspect gastric cancer, glioblastoma and pancreatic adenocarcinoma. silencing studies revealed required for cell survival, proliferation evasion senescence vitro growth vivo . Gain of- function showed high levels promote...
Chaperone-mediated autophagy (CMA) is a homeostatic process essential for the lysosomal degradation of selected subset proteome. CMA activity directly depends on levels LAMP2A, critical receptor substrate proteins at membrane. In glioblastoma (GBM), most common and aggressive brain cancer in adulthood, high LAMP2A tumor tumor-associated pericytes have been linked to temozolomide resistance progression. However, role hence CMA, any stem cell type or cells (GSC) remains unknown. this work, we...
Abstract A group of organotin(IV) complexes were prepared: [SnCy 3 (DMNI)] ( 1 ), (BZDO)] 2 (DMFU)] and [SnPh (BZDO) ] 4 for which DMNIH=2,6‐dimethoxynicotinic acid, BZDOH=1,4‐benzodioxane‐6‐carboxylic DMFUH=2,5‐dimethyl‐3‐furoic acid. The cytotoxic activities compounds – tested against pancreatic carcinoma (PANC‐1), erythroleukemia (K562), two glioblastoma multiform (U87 LN‐229) human cell lines; they show very high antiproliferative activity, with IC 50 values in the 150–700 n M range...
Abstract The cancer stem cell (CSC) hypothesis proposes a hierarchical organization of tumors, in which stem-like cells sustain tumors and drive metastasis. molecular mechanisms underlying the acquisition CSCs metastatic traits are not well understood. SOX9 is transcription factor linked to maintenance commonly overexpressed solid cancers including colorectal cancer. In this study, we show that levels higher (SW620) than primary (SW480) derived from same patient. This elevated expression...
The antitumor activity of the histone deacetylase inhibitors was tested in three well-characterized pancreatic adenocarcinoma cell lines, IMIM-PC-1, IMIM-PC-2, and RWP-1. These lines have been previously characterized terms their origin, status relevant molecular markers for this kind tumor, resistance to other antineoplastic drugs, expression differentiation markers. In study, we report that induce apoptosis cancer independently intrinsic conventional agents. inhibitor-induced is due a...
Selumetinib (AZD6244, ARRY-142886) is a MEK1/2 inhibitor that has gained interest as an anti-tumour agent. We have determined the degree of sensitivity/resistance to in panel colorectal cancer cell lines using proliferation and soft agar assays. Sensitive underwent G1 arrest, whereas had no effect on cycle resistant cells. Some showed high levels ERK1/2 phosphorylation absence serum. inhibited RSK AKT both sensitive Furthermore, mutations KRAS, BRAF, or PIK3CA were not clearly associated...
The impairment of the activity brain is a major feature aging, which coincides with decrease in function neural stem cells. We have previously shown that an extra copy regulated Ink4/Arf and p53 activity, s-Ink4/Arf/p53 mice, elongates lifespan delays aging. In this work, we examined physiology focusing on cell (NSC) population. show cells derived from old mice display enhanced neurosphere formation self-renewal compared wt controls. This correlates augmented expression Sox2, Sox9, Glast,...
Abstract Glioblastoma remains the most common and deadliest type of brain tumor contains a population self-renewing, highly tumorigenic glioma stem cells (GSCs), which contributes to initiation treatment resistance. Developmental programs participating in tissue development homeostasis re-emerge GSCs, supporting progression glioblastoma. SOX1 plays an important role neural progenitor pool maintenance. Its impact on glioblastoma largely unknown. In this study, we have found that high levels...
The use of heat shock protein 90 (Hsp90) inhibitors is an attractive antineoplastic therapy. We wanted to compare the effects benzoquinone 17-allylamino-17-demethoxygeldanamycin (17-AAG, tanespimycin) and novel isoxazole resorcinol–based Hsp90 inhibitor NVP-AUY922 in a panel pancreatic colorectal carcinoma cell lines primary cultures derived from tumors excised patients. PANC-1, CFPAC-1, Caco-2 cells were intrinsically resistant 17-AAG but sensitive NVP-AUY922. Other cellular models both...
Abstract The present study of inhibitors shows that the histone deacetylase–induced increase in P-glycoprotein (Pgp) mRNA (MDR1 mRNA) does not parallel either an Pgp protein or activity several colon carcinoma cell lines. Furthermore, studying polysome profile distribution, we show a translational control these In addition, MDR1 produced lines is shorter its 5′ end MCF-7/Adr (human breast carcinoma) and K562/Adr erythroleukemia) lines, both them expressing Pgp. different size due to use...