A5100397916- Heat shock proteins research
- Sperm and Testicular Function
- Peroxisome Proliferator-Activated Receptors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Adipose Tissue and Metabolism
- Physiological and biochemical adaptations
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Eicosanoids and Hypertension Pharmacology
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Medicinal Plants and Bioactive Compounds
- NF-κB Signaling Pathways
- Cell death mechanisms and regulation
- Intensive Care Unit Cognitive Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Hydrogen's biological and therapeutic effects
- Computational Drug Discovery Methods
- Nuclear Structure and Function
- Milk Quality and Mastitis in Dairy Cows
- Plant and fungal interactions
- Viral gastroenteritis research and epidemiology
- Insect symbiosis and bacterial influences
Gansu Agricultural University
2019-2025
West China Medical Center of Sichuan University
2006-2025
Third Xiangya Hospital
2024
Central South University
2017-2024
Beijing Obstetrics and Gynecology Hospital
2023
Capital Medical University
2021-2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2020-2023
Ministry of Education
2023
Ministry of Education of the People's Republic of China
2022
Qingdao Agricultural University
2021
Abstract Nuclear factor-κB (NF-κB), a survival signal induced by tumor necrosis factor (TNF), contributes substantially to the resistance TNF-induced cell death. Previous studies suggest that heat shock protein 90 (Hsp90) regulates stability and function of receptor-interaction proteins (RIP) IκB kinase β (IKKβ), key components NF-κB activation pathway. In this study, we showed Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin (17AAG) was synergistic with TNF induce apoptotic death in...
Freezing stress is one of the most detrimental environmental factors that can seriously impact growth, development, and distribution alfalfa (Medicago sativa L.). Exogenous salicylic acid (SA) has been revealed as a cost-effective method improving defense against freezing due to its predominant role in biotic abiotic resistance. However, how molecular mechanisms SA improve resistance still unclear. Therefore, this study, we used leaf samples seedlings pretreatment with 200 μM 0 SA, which...
Circular RNAs (circRNAs) have been shown to function in the reproductive systems including testis. However, their expression, as well testicular development of sheep remain undefined. Herein, we performed RNA sequencing reveal circRNA temporal expression patterns testes Tibetan from different stages maturation (3M, 3-month-old; 1Y, 1-year-old; 3Y, 3-year-old). A total 3,982, 414, and 4,060 differentially expressed (DE) circRNAs were uncovered 3M vs 1Y respectively. Functional enrichment...
Hsp90 is involved in correcting, folding, maturation and activation of a diverse array client proteins; it has also been implicated the treatment cancer recent years. In this work, comparative molecular field analysis (CoMFA), similarity indices (CoMSIA), docking dynamics were performed on three different series inhibitors to build 3D-QSAR models, which based ligand-based or receptor-based methods. The optimum models exhibited reasonable statistical characteristics with averaging internal q2...
In the oral epithelium, peripheral stores of Epstein-Barr virus (EBV) are transmitted from infiltrating B cells to epithelial cells. Once is cells, highly permissive nature this cell type for lytic replication allows amplification and exchange other hosts. Since initial transfer EBV requires transitioning B-cell a state that induces reactivation, we hypothesized there might be epithelium-specific signals allow sense appropriate environment initiate reactivation begin process. We previously...
Difenoconazole (DFZ) is a broad-spectrum triazole fungicide that widely utilized in agriculture. Although DFZ has been demonstrated to induce reproductive toxicity aquatic species, its toxic effects on the mammalian system have yet be fully elucidated. In vivo, male mice were administered 0, 20 or 40 mg/kg/d of via oral gavage for 35 days. Consequently, significantly decreased testicular organ coefficient, sperm count and testosterone levels, augmented malformation rates, elicited...
Continuous cropping obstacles pose significant constraints and urgent challenges in the production of Tussilago farfara L. This experiment investigated effects consecutive on T. over periods 1, 2, 3 years. It assessed yield quality flower buds, addition to physicochemical properties rhizosphere soil. The microbial community was analyzed through 16S rDNA ITS sequencing using Illumina Novaseq high-throughput technology, while also examining correlations among these factors. results reveal that...
Freezing stress is a major limiting environmental factor that affects the productivity and distribution of alfalfa (Medicago sativa L.). There growing evidence enhancing freezing tolerance through resistance-related genes one most efficient methods for solving this problem, whereas little known about complex regulatory mechanism stress. Herein, we performed transcriptome profiling leaves from two genotypes alfalfa, "Gannong NO.3" freezing-sensitive "WL326GZ" exposure to -10°C investigate...
Rodents respond to a chronic high-fat diet (HFD) in two ways: some readily become obese (obesity prone, OP) and others do not resistant, OR). Although several hypotheses have been proposed, the mechanisms underlying inter-individual susceptibility diet-induced obesity remain be fully defined. In present study, two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption ionisation time-of-flight MS was carried out for identification of differentially expressed...
One of the most studied and widely accepted conjectures aging process is oxidative stress theory. Previous studies have shown that salidroside can protect D-galactose-induced mouse model against a formulation Rhodiola rosea extracts (SHR-5) containing increases lifespan fruit fly. However, direct evidence linking itself with observed anti-aging effect in vivo relevant molecular mechanisms are poorly defined. In this study, we first demonstrated exhibited time-dependent effect, late-onset...
Two different mechanisms are considered to be related aging. Cumulative molecular damage caused by reactive oxygen species (ROS), the by-products of oxidative phosphorylation, is one these (ROS concept). Deregulated nutrient sensing insulin/insulin-like growth factor 1 (IGF-1) signaling (IIS) pathway second mechanism (IIS Temperature reduction (TR) known modulate aging and prolong life span in a variety organisms, but remain poorly defined. Here we first demonstrate that late-onset TR from...
BOLL is implicated in mammalian testicular function maintenance and spermatogenesis. To understand the expression patterns biological functions of sheep BOLL, we examined immunolocalization developing testes Small-Tail Han aged 0 days (D0), 2 months (2M), 5 (5M), 1 year (1Y), years (2Y), by qPCR, Western blot, immunohistochemistry methods. Firstly, morphological studies revealed that, addition to spermatogonia, ordered clear spermatocytes, as well round elongated spermatids sperm, were found...
Ark shells ( Scapharca subcrenata ) grown on the tidal flats are often exposed to high temperature stresses in summer. In order better understand their adaption extreme or natural temperature, we first determined 96-h upper lethal of ark shell and then investigated physiological transcriptional responses acute chronic thermal stress at median (32°C). A significantly higher cumulative mortality (52% 96 h) was observed heating treatment (AHT) group than that (22% 7 days) (CHT) group. The...
We have previously reported that glyoxalase domain-containing protein 4 (GLOD4) is expressed in sheep testes by proteome analysis, but its roles during testicular development remain unclear. The aim of this study was to understand the expression characteristics and biological functions GLOD4 gene developmental Tibetan testes. cDNA sequence cloned RT-PCR method, structural were analyzed using relevant bioinformatics software, including ProtParam, TMHMM, Signal P 4.1, SOPMA, phyre2. patterns...
The “ Photorhabdus virulence cassettes” (PVCs) secreted by are defined as “extracellular contractile injection systems” (eCISs) and can deliver effectors to eukaryotic hosts for cytotoxicity. Previously, we demonstrated the cryogenic electron microscopy (cryo-EM) structure assembly process of an intact PVC particle from asymbiotica . In this work, characterized biological functions a effector, which is homologous protein Ras/Rap1-specific endopeptidase domain (RRSP) in multifunctional...
Vascular remodeling is the fundamental basis for hypertensive disease, in which vascular smooth muscle cell (VSMC) dysfunction plays an essential role. Previous studies suggest that activation of peroxisome proliferator-activated receptor α (PPARα) by fibrate drugs has cardiovascular benefits independent lipid-lowering effects. However, underlying mechanism remains incompletely understood. This study explored role PPARα angiotensin II (Ang II)-induced and hypertension using VSMC-specific...
Beyond its well-known role in spermatogenesis and androgen production, mammalian testes are increasingly recognized as an immune-privileged organ for protecting autoantigenic germ cells, especially meiotic postmeiotic from systemic immune responses. Despite importance, the molecular mechanisms underlying this regulation mammals, including sheep, far known. In study, we searched genes associated with testicular privilege assessed their possible modulating by analyzing systematic profiling of...
Cerebral venous abnormalities, distinct from traditional arterial diseases, have been linked to brain atrophy in a previous community-based cohort study, specifically relation the reduction of deep medullary veins (r-DMVs). To better understand properties and biological functions serum extracellular vesicles (EVs) cerebral disease-associated atrophy, EVs are extracted both participants with r-DMV normal controls analyzed their proteomic profiles using Tandem Mass Tag label quantitation...