A5040376689- Computational Drug Discovery Methods
- Analytical Chemistry and Chromatography
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Metabolomics and Mass Spectrometry Studies
- Traditional Chinese Medicine Analysis
- Plant-based Medicinal Research
- Chromatography in Natural Products
- Advanced Biosensing Techniques and Applications
- Identification and Quantification in Food
- Cancer, Lipids, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Pharmaceutical Quality and Counterfeiting
- Protein purification and stability
- Nicotinic Acetylcholine Receptors Study
- Hemoglobinopathies and Related Disorders
- Iron Metabolism and Disorders
- Phytochemical Studies and Bioactivities
- Synthesis and biological activity
- Phytochemistry and Biological Activities
- Medicinal Plant Pharmacodynamics Research
- Flavonoids in Medical Research
- Pesticide Residue Analysis and Safety
- Toxin Mechanisms and Immunotoxins
- Immune Response and Inflammation
Shanghai Business School
2025
Second Military Medical University
2015-2024
Shanghai Research Institute of Chemical Industry
2022
Cell membrane chromatography (CMC) derived from pathological tissues is ideal for screening specific components acting on diseases complex medicines owing to the maximum simulation of in vivo drug-receptor interactions. However, there are no tissue-derived CMC models that have ever been developed, as well visualized affinity comparison potential active between normal and columns. In this study, a novel comparative normal/failing rat myocardium analysis system based online column selection...
Cell membrane affinity chromatography has been widely applied in protein (MP)-targeted drug screening and interaction analysis. However, current methods, the MP sources are derived from cell lines or recombinant expression, which time-consuming for culture purification, also difficult to ensure purity consistent orientation of MPs chromatographic stationary phase. In this study, a novel situ synthesis (iSMAC) method was developed utilizing cell-free expression (CFE) covalent immobilized...
Elucidating the active components of traditional Chinese medicine (TCM) is essential for understanding mechanisms TCM and promote its rational use as well TCM-derived drug development. Recent studies have shown that surface plasmon resonance (SPR) technology promising in this field. In present study, we propose an SPR-based integrated strategy to screen analyze major TCM. We used Radix Paeoniae Alba (RPA) example identify compounds can account anti-inflammatory mechanism via tumor necrosis...
Sorafenib is the first-line therapeutic agent for hepatocellular carcinoma (HCC), but drug resistance has become a major impediment. Previously we found that abnormal iron metabolism in HCC led to deficiency, whether it induces sorafenib during treatment of not yet disclosed. In this study, observed effects deficiency on and explored underlying mechanisms. The results revealed killing cells were weakened by effectively restored re-supplementation. ferroptosis indicators, including contents...
A surface plasmon resonance (SPR) biosensor-based active ingredients recognition system (SPR-AIRS) was developed, validated, and applied to screen signal transducer activator of transcription 3 (STAT3) ligands. First, features the screening were investigated in four aspects: (1) specificity STAT3-immobilized chip, it shows that chip could be STAT3 ligands from complex mixture; (2) linearity limit detection (LOD) system, minimum recovery cycle number determined as 5 cycles; (3) saturability...
According to the Chinese Pharmacopoeia 2015, only processed Aconitum tubers can be clinically applied, and effect of processing is unclear. This research aimed explore on cardiac efficacy alkaloids in tubers. First, chemical ingredients unprocessed were identified compared by using high performance liquid chromatography time-of-flight mass spectrometry (HPLC-TOF/MS) multivariate pattern recognition methods. Then representative tubers, aconitine, benzoylaconine, aconine, which belong...
Therapeutic drug monitoring (TDM) has played an important role in clinical medicine for precise dosing. Currently, chromatographic technology and immunoassay detection are widely used TDM have met most of the needs therapy. However, some problems still exist practical applications, such as complicated operation influence endogenous substances. Surface plasmon resonance (SPR) been applied to detect concentrations small molecules, including pesticide residues crops antibiotics milk, which...
Abstract Second near‐infrared (NIR‐II) fluorescence imaging has emerged as a breakthrough technology for accurately revealing complex mechanisms in vivo owing to its high sensitivity, deeper tissue penetration, spatiotemporal resolution, and throughput. This review provides comprehensive overview of NIR‐II imaging, specifically focusing on the materials used, including single‐walled carbon nanotubes (SWCNTs), quantum dots (QDs), rare‐earth nanoparticles (RENPs), organic fluorophores (OFs)....
The cellular target of matrine is identified.
Membrane proteins (MPs) are playing important roles in several biological processes. Screening new candidate compounds targeting MPs is for drug discovery. However, it remains challenging to characterize the interactions between and small-molecule ligands a label-free method. In this study, surface plasmon resonance (SPR)-based membrane protein-targeted active ingredients recognition strategy was constructed. This contains two major modules: affinity detection module ligand screening module....
Herbal medicines have long been widely used in the treatment of various complex diseases China. However, active constituents and therapeutic mechanisms many herbal remain undefined. Therefore, identification components target proteins these is a formidable task medicine research. In this study, we proposed strategy, which integrates network pharmacology with biomedical analysis surface plasmon resonance (SPR) to predict ingredients potential targets Sophora flavescens or Kushen Chinese,...
Candida albicans, the most common fungal pathogen related to colonization and biofilm formation on surfaces of indwelling medical devices, shows high resistance commonly used antifungal drugs. In this study, an ion-pairing chromatography-porous graphitic carbon column coupled with a time-of-flight mass spectrometry (IP-PGC-TOF/MS) system was developed for targeted untargeted profiling metabolites involved in planktonic growth C. albicans. Using analysis, 16 differential were screened...