A5101591931- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- Animal Disease Management and Epidemiology
- Neonatal Respiratory Health Research
- Viral Infections and Vectors
- Herpesvirus Infections and Treatments
- Respiratory Support and Mechanisms
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- vaccines and immunoinformatics approaches
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Parvovirus B19 Infection Studies
- Congenital Diaphragmatic Hernia Studies
- Plant Virus Research Studies
- Graphene and Nanomaterials Applications
- Cancer, Lipids, and Metabolism
- Hepatitis B Virus Studies
- Vascular Malformations Diagnosis and Treatment
- Neurological Complications and Syndromes
- Trigeminal Neuralgia and Treatments
Shanghai Veterinary Research Institute
2015-2024
Chinese Academy of Agricultural Sciences
2015-2024
Huanghuai University
2015-2024
Qujing Normal University
2022-2024
Qingdao University
2013-2022
Qingdao Municipal Hospital
2014-2022
Yunnan Maternal and Child Health
2020-2022
Innovation Team (China)
2019-2022
Anhui Agricultural University
2020
Ningbo Center for Disease Control and Prevention
2020
Current studies have revealed the immune effects of graphene oxide (GO) and utilized them as vaccine carriers adjuvants. However, GO easily induces strong oxidative stress inflammatory reaction at site injection. It is very necessary to develop an alternative adjuvant based on derivatives for improving responses decreasing side effects. Carnosine (Car) outstanding safe antioxidant. Herein, feasibility efficiency ultrasmall decorated with carnosine were explored. OVA@GO-Car was prepared by...
AIM:To develop a simple and convenient method for extracting genomic DNA from intestinal microflora enterobacterial repetitive intergenic consensus (ERIC)-PCR detection. METHODS:Five methods of bacterial DNA, including Tris-EDTA buffer, chelex-100, ultrapure water, 2% sodium dodecyl sulfate 10% Triton-100 with without sonication, were compared the commercial fecal extraction kit method, which is considered as gold standard extraction.The comparison was based on yield purity indexes structure...
The NASONE (Nasal Oscillation Post-Extubation) trial showed that noninvasive high-frequency oscillatory ventilation (NHFOV) slightly reduces the duration of invasive mechanical (IMV) in preterm infants, whereas NHFOV and intermittent positive pressure (NIPPV) result fewer reintubations than nasal continuous airway (NCPAP). It is unknown whether similarly effective extremely neonates or those with more severe respiratory failure (based on previous CO2 levels).To clarify better NIPPV NCPAP...
ABSTRACT It has been reported that lentogenic Newcastle disease virus (NDV) isolates have the potential to become velogenic after their transmission and circulation in chickens, but underlying mechanism is unclear. In this study, a highly NDV variant, JS10-A10, was generated from duck-origin isolate JS10 through 10 consecutive passages chicken air sacs. The properties of selected variant were determined using mean death time (MDT) assays, intracerebral pathogenicity index (ICPI), intravenous...
The present study was designed and conducted to compare the efficacy between nasal continuous positive airway pressure (NCPAP), intermittent positive-pressure ventilation (NIPPV), noninvasive high-frequency oscillatory (NHFOV) as primary in preterm infants with respiratory distress syndrome (RDS).This multicenter retrospective cohort performed using data from four tertiary neonatal intensive care units (NICUs) China 2016 2018. 512 RDS who received early non-invasive (NIV) were analyzed....
Abstract Background Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, it severely affects geese production worldwide. The objective of this study was to develop fluorescent quantitative real-time polymerase chain reaction (PCR) (FQ-PCR) assay for fast accurate quantification GPV DNA infected goslings, which can aid the understanding regular distribution pattern nosogenesis vivo. Results detection limit 2.8 × 10 1 standard copies,...
To evaluate clinical response to initial corticosteroid (CS) treatment in Chinese ulcerative colitis patients (UC) and identify predictors of response.Four hundred twenty-three UC who were initially treated with oral or intravenous CS from 2007 2011 retrospectively reviewed at eight inflammatory bowel disease centers China, 101 consecutive cases one-year follow-up analyzed further for predictors. Short-term outcomes within one month classified as primary non-response. Long-term year...
Rabbit hemorrhagic disease virus (RHDV), the causative agent of rabbit disease, is an important member caliciviridae family. Currently, no suitable tissue culture system available for proliferating RHDV, limiting study pathogenesis RHDV. In addition, mechanisms underlying RHDV translation and replication are largely unknown compared with other viruses. The replicon recently constructed in our laboratory provides appropriate model to without vitro propagation culture. Using this replicon, we...
Toll-like receptors (TLRs) play an important role in detecting pathogen-associated molecular patterns (PAMPs). Among the TLRs, TLR3 is involved recognition of double-stranded RNA. This study was designed to explore relationship between duTLR3 and duck reovirus (DRV) infection. In this study, we cloned performed a characterization complete sequence Peking (duTLR3). The expression level also determined, along with relative levels Mx IFN-α mRNA after DRV gene 2776-bp long encodes...
The fact that rabbit hemorrhagic disease virus (RHDV), an important member of the Caliciviridae family, cannot be propagated in vitro has greatly impeded progress investigations into mechanisms pathogenesis, translation, and replication this related viruses. In study, we have successfully bypassed obstacle by constructing a mutant RHDV (mRHDV) using reverse genetics technique. By changing two amino acids (S305R,N307D), produced specific receptor-recognition motif (Arg-Gly-Asp; called RGD) on...
Abstract Novel duck reovirus (NDRV) disease emerged in China 2011 and continues to cause high morbidity about 5.0 50% mortality ducklings. Currently there are no approved vaccines for the virus. This study aimed assess efficacy of a new vaccine created from baculovirus sigma C gene against NDRV. In this study, recombinant containing was constructed, purified protein used as candidate The estimated according humoral immune responses, cellular response protection NDRV challenge. results showed...
The present study aimed to investigate the effects of propofol on neonatal acute lung injury (ALI) in a rat model and examine molecular mechanisms underlying function. A ALI was established by intraperitoneal injection lipopolysaccharides (LPS). rats were treated with various concentrations score assessed. protein expression levels pro‑inflammatory cytokines detected using ELISA. In study, oxidative stress determined measuring level malondialdehyde (MDA) activity superoxide dismutase (SOD)...
Currently, both goose astrovirus (GoAstV) and goose-origin Newcastle disease virus (NDV) are widely infectious agents for goslings. There is no vaccine GoAstV. Capsid protein can elicit a neutralizing antibody in human astroviruses (HAstV). Molecular analysis of the genomic region encoding capsid protein(ORF2) has revealed that it contains epitopes. Goose-origin NDV also an agent. A wide range strains exist be commonly used as vectors. In present study, fusion cleavage site RRQKR↓F backbone...
A multilocus GWAS was performed to explore the genetic architecture of four growth traits in yak. In total, 354 female yaks for which measurements body weight (BW), withers height (WH), length (BL) and chest girth (CG) at weaning were available underwent genotyping with Illumina BovineHD BeadChip (770K). After quality control, we retained 98 688 SNPs animals analysis. We identified seven, 18, seven nine (corresponding 17, eight candidate genes) associated BW, WH, BL CG respectively....
A cell is the basic unit of life, and death inevitable for any cell. However, cancer cells that deviate from normal track can resist survive. Ferroptosis recently discovered as a modulated different other known forms in morphology, biochemistry, genetics. It characterized by iron-dependent lipid peroxidation regulated various metabolic pathways. The incidence mortality genitourinary system have been increasing recently. Although clinical practice therapy techniques improved, no plan with...