A5026834716- Pancreatic function and diabetes
- Congenital Diaphragmatic Hernia Studies
- Digestive system and related health
- Gut microbiota and health
- Endoplasmic Reticulum Stress and Disease
- Biochemical and Molecular Research
- Infant Nutrition and Health
- Neonatal Respiratory Health Research
- Peptidase Inhibition and Analysis
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- 14-3-3 protein interactions
- Hernia repair and management
- Diet and metabolism studies
- Acute Lymphoblastic Leukemia research
- Cancer-related Molecular Pathways
- Agriculture Sustainability and Environmental Impact
- Cancer, Hypoxia, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Renal function and acid-base balance
- Neurological Disorders and Treatments
- Cerebrospinal fluid and hydrocephalus
- Protein Kinase Regulation and GTPase Signaling
- Assisted Reproductive Technology and Twin Pregnancy
- Birth, Development, and Health
- Sleep and related disorders
University of Cincinnati Medical Center
2022-2024
Cincinnati Children's Hospital Medical Center
2019-2024
Sabin Vaccine Institute
2022
The gut microbiota, via the production of metabolites entering circulation, plays a role in blood pressure regulation. Blood is also affected by characteristics sleep. To date, no studies have examined relationships among microbiota/metabolites, pressure, and We hypothesized that fragmented sleep associated with elevated mean arterial an altered dysbiotic microbial community, changes fecal metabolites. In our model system, rats were randomized to 8 h fragmentation during rest phase (light...
Background & AimsThe intestinal stem cell niche is exquisitely sensitive to changes in diet, with high-fat caloric restriction, and fasting resulting altered crypt metabolism function. Unlike cells on the villus, are not immediately exposed dynamically changing contents of lumen. We hypothesized that enteroendocrine (EECs), which sense environmental cues response release hormones metabolites, essential for relaying luminal nutritional status animal deep crypt.MethodsWe used...
Maternal seeding of the microbiome in neonates promotes a long-lasting biological footprint, but how it impacts disease susceptibility early life remains unknown. We hypothesized that feeding butyrate to pregnant mice influences newborn's biliary atresia, severe cholangiopathy neonates. Here, we show administration mothers renders newborn resistant inflammation and injury bile ducts improves survival. The prevention hepatic immune cell activation survival trait is linked fecal signatures...
Iron overload disorder (IOD) affects many wildlife species cared for ex situ. Two of the four rhinoceros in human care, Sumatran (Dicerorhinus sumatrensis) and black (Diceros bicornis), are susceptible, whereas other two, white (Ceratotherium simum) greater one-horned (GOH) (Rhinoceros unicornis), relatively resistant to IOD. Complex interrelationships exist between mammalian hosts, their indigenous gut microbiota, metabolome, physical condition, iron availability. The goal this study was...
Congenital diaphragmatic hernia (CDH) is characterized by the herniation of abdominal contents into thoracic cavity during fetal period. This competition for space results in lung hypoplasia and vascular maldevelopment that can generate severe pulmonary hypertension (PH). The detailed mechanisms CDH pathogenesis are yet to be understood. Acknowledgment metabolism in-utero development help discern pathophysiology changes. Timed-pregnant dams received nitrofen or vehicle (olive oil) on E9.5...
Acute kidney injury (AKI) is common in critically ill patients, and sepsis its leading cause. Sepsis-associated AKI (SA-AKI) causes greater morbidity mortality than other etiologies, yet the underlying mechanisms are incompletely understood. Metabolomic technologies can characterize cellular energy derangements, but few discovery analyses have evaluated metabolomic profile of SA-AKI. To identify metabolic derangements amenable to therapeutic intervention, we assessed plasma urine metabolites...
Ras GTPases in complex with Guanosine triphosphate (GTP) or GTP analog exhibit dynamic equilibrium between two interconvertible conformations-an inactive state 1 and an active 2. Unlike Ras, it remains unclear if the GTP-bound form of Rho also exhibits multiple conformational states. Here, we describe a protocol for structural biochemical analyses RhoA GTPase. This can be adapted characterization other GTPases. For details on use execution this protocol, please refer to Lin et al. (2021).
Oncogenic kinase inhibitors show short-lived responses in the clinic due to high rate of acquired resistance. We previously showed that pharmacologically exploiting oncogene-induced proteotoxic stress can be a viable alternative oncogene-targeted therapy. Here, we performed extensive analyses transcriptomic, metabolomic and proteostatic perturbations during course treatment Her2+ breast cancer cells with Her2 inhibitor covering drug response, resistance, relapse withdrawal phases. found...
Fetuses undergo major surgical stress as well fluid shifts secondary to both twin-twin transfusion (TTTS) the fetoscopic surgery for treatment of TTTS. While pathophysiology TTTS is understood, acute metabolic changes that fetuses experience from are not. We sought evaluate in recipient metabolomic profile surgery. Amniotic was collected at beginning and end four cases performed 12/2022-2/2023. Samples were immediately processed evaluated via NMR-based Metabolomics Facility protocol. In...
Spina bifida, known more commonly as myelomeningocele, is a neural tube defect that results in herniation of the cerebellum through foramen magnum into central canal part Chiari II malformation. Effects stemming from herniated and its metabolic profile have not been extensively studied. The objective this study to examine effects disease on utero utilization retinoid acid-induced bifida rat model. Analysis model at mid-late (day 15) term 20) gestation comparison both non-exposed retinoic...
Abstract Background and aims The intestinal stem cell niche is exquisitely sensitive to changes in diet, with high fat caloric restriction, fasting resulting altered crypt metabolism function. Unlike cells on the villus, are not immediately exposed dynamically changing contents of lumen. We hypothesized that enteroendocrine (EECs), which sense environmental cues response release hormones metabolites, essential for relaying nutrient status animal deep crypt. Methods used tamoxifen-inducible...
Abstract Oncogenic kinase inhibitors show short-lived responses in the clinic due to high rate of acquired resistance. We previously showed that pharmacologically exploiting oncogene-induced proteotoxic stress can be a viable alternative oncogene-targeted therapy. Here, we performed extensive analyses transcriptomic, metabolomic and proteostatic perturbations during course treatment Her2+ breast cancer cells with Her2 inhibitor covering drug response, resistance, relapse withdrawal phases....