A5102952921- Cardiac, Anesthesia and Surgical Outcomes
- Extracellular vesicles in disease
- Moyamoya disease diagnosis and treatment
- Biochemical and Molecular Research
- Cancer, Hypoxia, and Metabolism
- Intracranial Aneurysms: Treatment and Complications
- Hepatocellular Carcinoma Treatment and Prognosis
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Atrial Fibrillation Management and Outcomes
- Bioactive Compounds and Antitumor Agents
- Neuroinflammation and Neurodegeneration Mechanisms
- Neurological Complications and Syndromes
- S100 Proteins and Annexins
- Cardiac and Coronary Surgery Techniques
- MicroRNA in disease regulation
- Cardiovascular Disease and Adiposity
- Spinal Cord Injury Research
- Genomics, phytochemicals, and oxidative stress
- Cardiovascular Health and Risk Factors
- Inflammasome and immune disorders
- vaccines and immunoinformatics approaches
- Image Processing Techniques and Applications
- Redox biology and oxidative stress
- Biochemical and Structural Characterization
- Child and Adolescent Psychosocial and Emotional Development
Liaocheng People's Hospital
2020-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2021-2025
Shandong University
2019-2024
Hubei University
2021-2024
Zhongshan Hospital
2021-2024
Fu Wai Hospital
2024
State Key Laboratory of Cardiovascular Disease
2024
Shandong Provincial Hospital
2020-2024
Nanjing University of Aeronautics and Astronautics
2024
Fudan University
2021-2024
Remnant cholesterol (remnant-C) contributes to atherosclerotic cardiovascular disease (ASCVD), particularly in individuals with impaired glucose metabolism. Patients metabolism and ASCVD remain at significant residual risk after coronary artery bypass grafting (CABG). However, the role of remnant-C this population has not yet been investigated. Adult patients prediabetes or diabetes undergoing isolated CABG were consecutively enrolled a longitudinal cohort between 2013 2018. The impact on...
Exosomes are reported to mediate several disease-related microRNAs (miRNAs) affect the progression of diseases, including atherosclerosis.Here, we aimed screen atherosclerosis-associated miRNAs and preliminarily investigate potential regulatory mechanism atherosclerosis.First, lesion model for human umbilical vein endothelial cells (HUVECs) was favorably constructed.Later, through RNA-sequencing bioinformatics analyses, miR-342-5p identified in HUVECs.MiR-342-5p overexpression or knockdown...
Stress hyperglycemia ratio (SHR) is recognized as a reliable indicator of acute during stress. Patients undergoing coronary artery bypass grafting (CABG) are at high risk stress hyperglycemia, but little attention has been paid to this population. This study the first investigate association between SHR and both short-term long-term prognosis in CABG patients, with further exploration impact across different glucose metabolic states. A total 18,307 patients isolated were consecutively...
IL-10-producing regulatory B cells (IL-10 + cells), a dominant cell (Breg) subset, foster tumor progression. However, the mechanisms underlying their generation in HCC are poorly understood. Ten-eleven translocation-2 (TET2), predominant epigenetic enzyme cells, regulates gene expression by catalyzing demethylation of 5-methylcytosine into 5-hydroxymethyl cytosine (5hmC). In this study, we investigated role TET2 IL-10 and its prospects for clinical application.TET2 activation triggered...
Cerebral ischemia-reperfusion injury (CIRI) can cause neuronal apoptosis and lead to irreversible brain injury. Numerous lncRNAs have been reported play important roles in CIRI, but it is unclear whether these function through exosomes.
N-glycosylation-modified immunoglobulin G (IgG) is crucial for managing the inflammatory response balance and significantly influences progression of many disorders. IgG N-glycosylation has been demonstrated to correlate with risk factors moyamoya disease (MMD), such as hypertension, diabetes, dyslipidemia. This research aimed evaluate diagnostic efficacy MMD. Ultra-high-performance liquid chromatography (UPLC) was employed examine properties N-glycans in blood samples from 116 patients MMD...
Background The gut microbiota (GM) is believed to be closely associated with symptomatic carotid atherosclerosis (SCAS), yet more evidence needed substantiate the significant role of GM in SCAS. This study, based on detection bacterial DNA plaques, explores characteristics SCAS patients plaque genetic material positivity, aiming provide a reference for subsequent research. Methods We enrolled 27 healthy individuals (NHF group) and 23 (PFBS group). utilized 16S rDNA V3-V4 region gene...
Abstract To explore the relationship between mental health of left-behind middle school students and awareness health. Using cluster sampling method to select 428 in Jinan, using scale self-compiled cognitive attitude questionnaire. We found students’ level force, paranoia, hostility interpersonal sensitivity, depression, anxiety, learning pressure, maladjustment, mood swings, psychological imbalance total score were significant difference compared with norm (t = 10.67, 3.86, 3.78, 7.54,...
Phospholipids are widely utilized in various industries, including food, medicine, and cosmetics, due to their unique chemical properties healthcare benefits. Phospholipase D (PLD) plays a crucial role the biotransformation of phospholipids. Here, we have constructed super-folder green fluorescent protein (sfGFP)-based phospholipase expression surface-display system Escherichia coli, enabling surface display sfGFP-PLDr34 on bacteria. The displayed showed maximum enzymatic activity at pH 5.0...
Background: Globally, ischemic stroke (IS) is ranked as the second most prevailing cause of mortality and considered lethal to human health. This study aimed identify genes pathways involved in onset progression IS. Methods: GSE16561 GSE22255 were downloaded from Gene Expression Omnibus (GEO) database, merged, subjected batch effect removal using ComBat method. The limma package was employed differentially expressed (DEGs), followed by enrichment analysis protein-protein interaction (PPI)...
In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to heat-resistant CL7 tag using SLOPE method, cloned into Escherichia coli Pichia pastoris vectors. Twenty E. ten P. vectors constructed. Expression supernatants heated, heteroproteins removed, fusion proteins purified by nickel affinity (Ni-NTA) chromatography. Fusion digested on column human rhinovirus (HRV) 3C protease, AMPs released further...
Adenosine triphosphate (ATP) and S-adenosyl-L-methionine (SAM) are important intermediates that widely present in living organisms. Large-scale preparation application of ATP or SAM is limited by expensive raw materials. To lower the production costs for ATP/SAM, this study we used strategies applying engineered multidomain scaffold proteins to synthesize SAM. An artificial protein containing CBM3 domain, IM CL-labeled was assembled form complex 1 catalytic reactions increase production. The...
While acknowledging carotid atherosclerosis (CAS) as a risk factor for ischemic stroke, reports on its pathogenesis are scarce. This study aimed to explore the potential mechanism of CAS through RNA-seq data analysis. Carotid intima tissue samples from patients and healthy subjects were subjected analysis, which yielded, 1,427 differentially expressed genes (DEGs) related CAS. Further, enrichment analysis (Gene Ontology, KEGG pathway, MOCDE analysis) was performed DEGs. Hub identified via...
Background: Oxidative stress and endoplasmic reticulum are important components of the cellular process, which plays a critical role in tumor initiation progression. Methods: First, correlation between oxidative was detected 68 human hepatocellular carcinoma (HCC) tissue microarray samples by immunohistochemistry. Differentially expressed stress- stressrelated genes (OESGs) then were screened HCC. Next, an OESGs prognostic signature constructed for HCC training cohort (TCGA-LIHC from The...
<title>Abstract</title> <bold>Background: </bold>Bypass surgery is a leading treatment strategy for moyamoya disease. Antiplatelet therapy (APT) has the potential to prevent thrombosis and possibly enhance bypass graft patency. However, efficacy safety of APT following remain debatable. This study aims assess clinical outcomes after surgery. <bold>Methods: </bold>A systematic review was conducted evaluate impact associated complications Electronic databases, including PubMed, Embase,...
DNA polymerases are important enzymes that synthesize molecules and therefore critical to various scientific fields as essential components of in vitro synthesis reactions, including PCR. Modern diagnostics, molecular biology, genetic engineering require with improved performance. This study aimed obtain characterize a new CL7-Taq fusion polymerase, which the coding sequence Taq polymerase was fused CL7, variant CE7 (Colicin E7 DNase) from Escherichia coli. The resulting novel recombinant...
This study aims to establish a prognostic nomogram for patients who underwent transarterial chemoembolization (TACE) recurrent hepatocellular carcinoma (HCC) after hepatectomy.
Cardiac surgery-associated acute kidney injury (CSA-AKI) is prevalent and increasingly reported. Its diagnosis traditionally follows the Kidney Disease: Improving Global Outcomes (KDIGO) AKI criteria. However, little evidence supports its appropriateness for cardiac surgery patients, particularly regarding subtle serum creatinine change (ΔSCr) that defines mild AKI.
Background: Biomarkers like coronary artery calcium (CAC) and traditional risk factors are well-validated for disease (CAD) assessment but not always available, with limited workup efficiency potential radiation risk. Facial features contain biological information related to atherosclerosis. Aims: We aim extract CAC factor-associated digital biomarkers from facial images evaluate their value in predicting CAD status compared conventional clinical approaches. Methods: Suspected individuals...