A5041200972- Neuroblastoma Research and Treatments
- Biomarkers in Disease Mechanisms
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Congenital heart defects research
- Circular RNAs in diseases
- Extracellular vesicles in disease
- Traumatic Brain Injury and Neurovascular Disturbances
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Atherosclerosis and Cardiovascular Diseases
- Photodynamic Therapy Research Studies
- Renal and related cancers
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
- 3D Printing in Biomedical Research
- Cholesterol and Lipid Metabolism
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Signaling Pathways in Disease
- Neuropeptides and Animal Physiology
- Reproductive System and Pregnancy
- Neurogenesis and neuroplasticity mechanisms
- Viral Infections and Immunology Research
Lovelace Respiratory Research Institute
2021-2025
Biomedical Research Institute
2021-2025
Candiolo Cancer Institute
2011-2024
Mind Research Network
2023
University of Turin
2008-2021
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2020
Azienda Ospedaliero Universitaria San Giovanni Battista
2012
Mario Negri Sud Foundation
2012
Centre National de la Recherche Scientifique
2009
Sorbonne Université
2009
Article27 December 2018Open Access Source DataTransparent process TFEB controls vascular development by regulating the proliferation of endothelial cells Gabriella Doronzo Corresponding Author [email protected] orcid.org/0000-0002-3693-8178 Department Oncology, University Turin, Candiolo, Italy Candiolo Cancer Institute-FPO-IRCCS, Search for more papers this author Elena Astanina Davide Corà Translational Medicine, Piemonte Orientale University, Novara, Giulia Chiabotto Medical Sciences,...
Liver X receptors (LXRα, LXRβ) are master regulators of cholesterol homeostasis. In the endothelium, perturbations cell have an impact on fundamental processes. We, therefore, assessed effects LXR activation endothelial functions related to angiogenesis in vitro and vivo.LXR agonists (T0901317, GW3965) blunted migration, tubulogenesis, proliferation human umbilical vein cells. By affecting homeostasis, impaired compartmentation vascular growth factor receptor-2 lipid rafts/caveolae led...
Angiogenesis requires the temporal coordination of proliferation and migration endothelial cells. Here, we investigated regulatory role microRNAs (miRNAs) in harmonizing angiogenesis processes a three-dimensional vitro model. We described microRNA network which contributes to observed down- upregulation proliferative migratory genes, respectively. Global analysis miRNA–target gene interactions identified two sub-network modules, first organized upregulated miRNAs connected with downregulated...
The tyrosine kinase encoded by the MET oncogene is activated gene mutation or amplification in tumors, which most instances maintain addiction, i.e., dependency, to activation. This makes an attractive candidate for targeted therapies. Here we show that, 3/3 MET-addicted human gastric cancer cell lines, inhibition resulted a 3- 4-fold increased expression of antiapoptotic small heat-shock protein 27 kDa (HSP27, HSPB1). HSP27 increase depended on MEK/ERK pathway and factor 1 (HSF1)...
Enterovirus D68 (EV-D68) is a non-polio enterovirus that causes respiratory illness and linked to acute flaccid myelitis (AFM) in infants children. Recent demonstration of association EV-D68 with extracellular vesicles (EVs) released from infected cells vitro suggests role for these non-lytic dissemination virus beyond the tract. We previously reported permissiveness cotton rat (Sigmodon hispidus) infection different strains clades A B, but did not investigate EVs. present model clinical...
Semen-derived extracellular vesicles (SEVs) have been shown to inhibit transactivation of the long terminal repeat (LTR) in human immunodeficiency virus type 1 (HIV-1, or HIV) and, hence, viral replication by blocking interaction transcriptional activator Tat and host transcription factors NF-kB Sp1. The ability SEVs regulate activities suggests that may contain activators repressors. Here, we identified proteins interacted with subunit p65. Integrative network pathway enrichment analyses...
Over sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly neuroscience, neurodegenerative diseases brain tumors. Glioblastoma is most lethal form cancer, with very limited treatment options poor prognosis. In this study on rats, we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitizer-free (PS-free-LT) using quantum-dot-based 1267 nm diode. This wavelength, highly absorbed oxygen,...
Neuronal pentraxins (NPTX) and their corresponding receptors (NPTXR) have been studied as synapse-associated proteins in the nervous system, but role cancer is largely unknown. By applying a multidisciplinary, high-throughput proteomic approach, we recently identified peptide ligand motif for targeted drug delivery to neuroblastoma. Here, report sequence similarity between this conserved portion of pentraxin domain that involved homo- hetero-oligomerization NPTX2 NPTXR. We show that,...
Traumatic brain injury (TBI) and severe blood loss resulting in hemorrhagic shock (HS) are each leading causes of mortality morbidity worldwide, present additional treatment considerations when they comorbid (TBI+HS) as a result competing pathophysiological responses. The current study rigorously quantified biomechanics with high precision sensors examined whether blood-based surrogate markers were altered general trauma well post-neurotrauma. Eighty-nine sexually mature male female Yucatan...
The observation that the architecture of cardiovascular and nervous systems is drawn by common guidance cues closeness between neural progenitors endothelial cells in vascular niche strongly suggests existence links cell fates. We identified an embryonic stem cell-derived discrete, nonclonal population expressing two growth factor receptors neuropilin-1 (Nrp1) Flk1 differentiates vitro toward or phenotypes depending on microenvironmental cues. When microinjected chick embryo, Nrp1(+)...
Glioblastomas (GBM) are the most aggressive tumors originating in brain. Histopathologic features include circuitous, disorganized, and highly permeable blood vessels with intermittent flow. These contribute to inability direct therapeutic agents tumor cells. Known targets for anti-angiogenic therapies provide minimal or no effect overall survival of 12-15 months following diagnosis. Identification novel therefore remains an important goal effective treatment vascularized such as GBM. We...
In the last several years, accumulating evidence indicates that noncoding RNAs, especially long-noncoding RNAs (lncRNAs) and microRNAs, play essential roles in regulating angiogenesis. However, contribution of lncRNA-mediated competing-endogenous RNA (ceRNA) activity control capillary sprouting from pre-existing ones has not been described so far. Here, by exploiting transcriptomic profile VEGF-A-activated endothelial cells a consolidate three-dimensional culture system, we identified list...
Graphical abstractAbstractInterferon-α (IFN-α) comprises a family of 13 cytokines involved in the modulation antiviral, immune, and anticancer responses by orchestrating complex transcriptional network. The activation IFN-α signaling pathway endothelial cells results decreased proliferation migration, ultimately leading to suppression angiogenesis. In this study, we knocked-down expression seven established or candidate modulators response reconstruct gene regulatory network investigate...
Abstract Embryonic stem cells (ES) are a valuable source of endothelial cells. By co-culturing ES with the stromal PA6 cells, commitment can be achieved by adding exogenous FGF2 or BMP4. In this work, molecular pathways that direct differentiation toward endothelium in response to evaluated and compared those activated To purpose genes expression profiles both ES/PA6 co-cultures pure cultures were obtained microarray technique at different time points. The bioinformatics processing data...
The connections existing between vessels and nerves go beyond the structural architecture of vascular nervous systems to comprise cell fate determination. analysis functional/molecular links that interconnect endothelial neural commitments requires a model in which two differentiation programs take place at same time an artificial controllable environment. To this regard, work presents vitro differentiate embryonic stem (ES) cells simultaneously into mature neurons cells. Murine ES are...
Abstract For sixty years, laser technologies have undergone a technological revolution and become one of the main tools in biomedicine, particularly neuroscience, neurodegenerative diseases brain tumours. Glioblastoma is most lethal form cancer with very limited treatment options poor prognosis. In this study on rats we demonstrate that glioblastoma (GBM) growth can be suppressed by photosensitiser-free laser-treatment (PS-free-LT) using quantum-dot based 1267 nm diode. This wavelength,...
Substance use disorder (SUD) significantly increases the risk of neurotoxicity, inflammation, oxidative stress, and impaired neuroplasticity. The activation inflammatory pathways by substances may lead to glial chronic neuroinflammation, potentially mediated release extracellular particles (EPs), such as condensates (ECs) vesicles (EVs). These particles, which reflect physiological, pathophysiological, metabolic states their cells origin, might carry molecular signatures indicative SUD. In...