A5077533634- Advanced biosensing and bioanalysis techniques
- Molecular Biology Techniques and Applications
- Glioma Diagnosis and Treatment
- Gene expression and cancer classification
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Advanced Fiber Optic Sensors
- Photonic and Optical Devices
- Biosensors and Analytical Detection
- Microfluidic and Capillary Electrophoresis Applications
- Herpesvirus Infections and Treatments
- Probiotics and Fermented Foods
- Poxvirus research and outbreaks
- 3D Printing in Biomedical Research
- Enterobacteriaceae and Cronobacter Research
- Extracellular vesicles in disease
- Viral gastroenteritis research and epidemiology
- Nanopore and Nanochannel Transport Studies
- Neonatal and Maternal Infections
- Bacillus and Francisella bacterial research
- Photonic Crystals and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Systemic Lupus Erythematosus Research
- Carbon and Quantum Dots Applications
- Cancer Genomics and Diagnostics
St. Michael's Hospital
2022-2025
Unity Health Toronto
2022-2024
Centre hospitalier de l'Université Laval
2005-2021
Centre Hospitalier Universitaire Sainte-Justine
2017
Centre hospitalier universitaire de Québec
2005-2014
Université Laval
2005-2012
Current hybridization protocols on microarrays are slow and need skilled personnel. Microfluidics is an emerging science that enables the processing of minute volumes liquids to perform chemical, biochemical, or enzymatic analyzes. The merging microfluidics microarray technologies constitutes elegant solution will automate speed up hybridization.We developed a microfluidic flow cell consisting network chambers channels molded into polydimethylsiloxane substrate. substrate was aligned...
ABSTRACT The presence of Enterococcus -associated vancomycin resistance genes vanA , vanB vanD vanE and vanG in rectal swabs was investigated two hospitals using PCR. All detected were associated with vancomycin-resistant enterococci (VRE), whereas VRE-associated only one hospital (4.7%). However, both hospitals, high prevalences (6.2 2.3%), (43.8 26.7%), (10.5 6.9%) not found.
Abstract The emergence of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) was met with rapid development robust molecular-based detection assays. Many SARS-CoV-2 molecular tests target multiple genetic regions the virus to maximize and protect against diagnostic escape. Despite relatively moderate mutational rate SARS-CoV-2, numerous mutations known negative impact on assays have been identified. In early 2021, we identified four samples positive for a nucleocapsid (N) gene drop...
In 2022, the global dissemination of mpox virus (MPXV) outside endemic regions prompted expansion diagnostic testing worldwide. This study assesses performance characteristics 5 real-time polymerase chain reaction (PCR) assays in detecting MPXV during 2022 outbreak.
Abstract We developed a single diagnostic assay to detect Glioma biomarkers including whole chromosomal and gene copy number variations (CNVs), nucleotide (SNVs). CNVs SNVs are traditionally detected using different large/high-throughput platforms that can only exist in big genomic facilities. This leads very high molecular costs long turnaround times. The current complex algorithm creates delays patient management while increasing inequity healthcare as smaller non-centralized labs unable...
The hybridization behavior of small oligonucleotides arrayed on glass slides is currently unpredictable. In order to examine the efficiency capture probes along target nucleic acid, 20-mer oligonucleotide were designed hybridize at different distances from 5′ end two overlapping 402- and 432-bp ermB products amplified DNA. These immobilized via their onto hybridized with labeled products. Evaluation signal for each probe revealed an inverse correlation length overhanging captured strand...
We explore a new calibration-free approach to biodetection based on whispering gallery modes (WGMs) without reference measure and relative shifts. Thus, the requirement keep track of sensor position is removed, freely moving population fluorophore-doped polystyrene microspheres can now fulfill this role sensing resonator. Breaking free from fixed surface-based biosensing promotes adhesion between microsphere sensors analytes since both be thoroughly mixed. The 70-nm-wide spectrum green...
aggregates the DNA nanospheres.After being spotted on a C18 reverse-phase silica thin-layer chromatography plate, aggregated nanospheres yield blue spot (positive) and monodisperse red (negative).We are currently able to detect 1 pmol/L HSV1-vector1 (6 ϫ 10 6 copies in L-reaction volume) after 4-min amplification at 55 °C (Fig. 1F) with good intra-and interassay reproducibility time °C.These results based 3 different experiments conducted days, each duplicate.Longer times produce nonspecific...
A structured oligonucleotide is irreversibly digested in the presence of its complementary target during PCR, releasing a short tag for microarray hybridization single vessel and reaction mixture.
We propose new parameters to characterize a whispering gallery mode spectrum, which can be regarded as reliable independent biodetection indicators for an instantaneous microsphere's spectrum. With these parameters, since there is no need spectral comparison analyte-free reference, three-dimensional stirring of fluorescent microspheres in fluidic setup allowed. Through analysis the presence bacterial spores, we found five insightful quantitative biodetection. Unveiled by this analysis,...
Hybridization of <italic>Streptococcus agalactiae cfb</italic> gene amplicons with bead-grafted capture probes is studied in relation to SNPs, dangling end's length, and presence non-specific DNA clinical samples.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) hospital outbreaks have been common and devastating during the disease 2019 (COVID-19) pandemic. Understanding SARS-CoV-2 transmission in these environments is critical for preventing managing outbreaks.Outbreak investigation through epidemiological mapping whole-genome sequencing phylogeny.Hospital in-patient medical unit outbreak Toronto, Canada, from November 2020 to January 2021.The involved 8 patients 10 staff was associated...
Abstract We aimed to develop a single streamlined assay detect all Glioma biomarkers. The current diagnosis of Gliomas incorporates molecular biomarkers that include whole chromosomal copy number variations (CNVs) and nucleotide (SNVs). Testing CNVs SNVs are traditionally done using different large platforms performed in genomic facilities have the suitable infrastructure. This creates delays testing, where samples undergo lengthy referrals tissue is often split between institutions, which...
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This study compared the Altona RealStar™ VZV Kit 1.0 real time quantitative PCR with in‐house qualitative conventional on cerebrospinal fluid, mucocutaneous, and other uncommon clinical specimens. Overall, positive negative agreement percentages were respectively 97.9% (95%CI: 93.8‐99.6), 100.0% 93.1‐100.0), 96.3% 89.4‐99.2) while Cohen's kappa statistic value was 0.96 0.91‐1.00). assay reported average viral loads of 4.4 × 10 5 1.1 7 copies/mL in fluid cutaneous specimens, ( P < 0.01)....
The World Health Organization (WHO) introduced molecular identifiers for the diagnosis and prognosis of CNS tumors including mutational status isocitrate dehydrogenase or IDHgenes in glial tumors. Currently used immunohistochemistry (IHC) is not capable detecting non-canonical mutations, sequencing often required as a follow-up. Current next-generation (NGS) technologies tumor marker detection introduce key challenges high capital cost, complex infrastructure requirements, long turnaround...