A5012619428- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Cell death mechanisms and regulation
- Cancer, Lipids, and Metabolism
- Natural product bioactivities and synthesis
- Phytochemicals and Antioxidant Activities
- interferon and immune responses
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Ferroptosis and cancer prognosis
- Cancer Cells and Metastasis
- Plant biochemistry and biosynthesis
- Metastasis and carcinoma case studies
- Genomics, phytochemicals, and oxidative stress
- Mathematical Biology Tumor Growth
- Flavonoids in Medical Research
- Phytochemical compounds biological activities
- Cancer, Stress, Anesthesia, and Immune Response
- Medicinal plant effects and applications
- Plant responses to elevated CO2
- Nanoplatforms for cancer theranostics
- Cell Adhesion Molecules Research
- Plant Stress Responses and Tolerance
- Bioactive natural compounds
- Plant Toxicity and Pharmacological Properties
University of Missouri
2022-2025
Gyeongsang National University
2017-2024
Gyeongsang National University Hospital
2019-2022
National Institute of Plant Genome Research
2016
In nature plants are often simultaneously challenged by different biotic and abiotic stresses. Although the mechanisms underlying plant responses against single stress have been studied considerably, tolerance under combined is not understood. Also, mechanism used to combat independently sequentially occurring many number of stresses has also systematically studied. From this context, in study, we attempted explore shared response sunflower independent using meta-analysis publically...
Emerging evidence suggests that breast cancer stem cells (BCSCs), and epithelial-mesenchymal transition (EMT) may be involved in resistance to doxorubicin. However, it is unlear whether the doxorubicin-induced EMT expansion of BCSCs related dormancy, or outgrowing with maintaining doxorubicin, phenotypes can transferred other doxorubicin-sensitive cells. Here, we characterized phenotype doxorubicin-resistant TNBC while monitoring process CSCs during establishment MDA-MB-231 human (DRM...
Abstract Introduction: Cisplatin (CDDP) and its analogs are widely used in lung cancer (LC) treatment. However, despite their effectiveness, advanced LC has a poor 5-year survival rate of less than 10% due to chemoresistance. This resistance is often driven by disrupting apoptosis, key hallmark cancer. Ferroptosis, an iron-dependent, non-apoptotic cell death pathway lipid peroxidation, presents promising alternative bypass apoptotic resistance. In this study, we developed novel,...
We previously demonstrated that anthocyanins from the fruits of Vitis coignetiae Pulliat (AIMs) induced apoptosis hepatocellular carcinoma cells. However, many researchers argued concentrations AIMs were too high for in vivo experiments. Therefore, we performed vitro at lower and experiments anti-cancer effects AIMs. inhibited cell proliferation Hep3B cells a dose-dependent manner with maximum concentration 100 µg/mL. also invasion migration µg/mL or without presence TNF-α. To establish...
Vitis coignetiae Pulliat (Meoru in Korea) has been used Korean folk medicine for the treatment of inflammatory diseases and cancers. Evidence suggests that NF-κB activation is mainly involved cancer cell proliferation, invasion, angiogenesis, metastasis. TNF-α also enhances process tumor development. Recently, flavonoids from plants have reported to inhibitory effects on activities. We investigated anthocyanins extracted fruits (AIM, isolated Meoru (AIM)) TNF-α-induced activities MCF-7 human...
Anthocyanins isolated from Vitis coignetiae Pulliat (Meoru in Korea) (AIMs) have various anti-cancer properties by inhibiting Akt and NF-κB which are involved drug resistance. Cisplatin (CDDP) is one of the popular agents. Studies reported that MCF-7 human breast cancer cells high resistance to CDDP compared other cell lines. In this study, we confirmed tested whether AIMs can overcome cells. Cell viability assay revealed were more resistant treatment than MDA-MB-231 exhibiting aggressive...
Breast cancer is one of the major causes deaths due to cancer, especially in women. The crucial barrier for breast treatment resistance radiation therapy, important local regional therapies. We previously established and characterized radio-resistant MDA-MB-231 cells (RT-R-MDA-MB-231 cells) that harbor a high expression stem (CSCs) EMT phenotype. In this study, we performed antibody array analysis identify hub signaling mechanism RT-R-MDA-MB-231 by comparing parental (p-MDA-MB-231) cells....
The Korean Petasites japonicus is a perennial plant used in folk medicine as remedy for many diseases and popularly consumed spring greens. Ten polyphenols were characterized from the leaves, stems roots of this via high-performance liquid chromatography-tandem mass spectrometry. Individual quantified first time using calibration curves six structurally related external standards. Validation data indicated that coefficients determinations (R2 ) ≥0.9702 all Recoveries measured at 50 100 mg/L...
c-Jun N-terminal kinase (JNK) is activated by chemotherapeutic reagents including natural plant polyphenols, and cell fate determined phospho-JNK as survival or death depending on stimuli types. The purpose of this study was to elucidate the role JNK anticancer effects Korean Artemisia annua L. (pKAL) polyphenols in p53 wild-type HCT116 human colorectal cancer cells. Cell morphology, protein expression levels, apoptosis/necrosis, reactive oxygen species (ROS), acidic vesicles,...
The anticancer effects of natural phytochemicals are relevant to the modulation cytokine signaling pathways in various cancer cells with stem-like properties as well immune cells. aim this study was elucidate a novel mechanism Artemisia annua L. polyphenols (pKAL) involved regulation growth factors, cytokines and mediators HCT116 colorectal Through RayBiotech human L-1000 antibody array bioinformatics analysis, we show here that pKAL-induced associated downregulation factor proteins...
Abstract Extracellular vesicles (EVs) hold potential as effective carriers for drug delivery, providing a promising approach to resolving challenges in lung cancer treatment. Traditional treatments, such with the chemotherapy cisplatin, encounter resistance standard cell death pathways like apoptosis, prompting need explore alternative approaches. This study investigates of iron oxide nanoparticles (IONP) and EVs induce ferroptosis—a regulated mechanism—in cells. We formulated novel EV...
Plant-derived natural polyphenols exhibit anticancer activity without showing any noticeable toxicities to normal cells. The aim of this study was investigate the role p53 on effect isolated from Korean Artemisia annua L. (pKAL) in HCT116 human colorectal cancer We confirmed that pKAL induced reactive oxygen species (ROS) production, propidium iodide (PI) uptake, nuclear structure change, and acidic vesicles a p53-independent manner p53-null cells through fluorescence microscopy analysis...
Small extracellular vesicles (sEVs) are cargo-carrying cellular nano-vesicles that have been explored for developing organic drug delivery modalities (DVMs), an alternative to synthetic liposomes.
Worldwide, liver cancer is the most frequent fatal malignancy. Liver prognosis poor because patients frequently receive advanced-stage diagnoses. The current study aimed to establish potential pharmacological targets and biological networks of scutellarein (SCU) in cancer, a natural product known have low toxicity side effects. To identify differentially expressed genes between SCU-treated SCU-untreated HepG2 cells, RNA sequencing (RNA-seq) was carried out. A total 463 were revealed...
Abstract Artemisia annua L., (Gaddongsook in Korean), an annual herb, has been used for a long time Korean folk medicine the treatment of infectious or chronic disorder In addition, it possesses anti-inflammatory, and anti-cancer activity. However, molecular mechanism properties is still unclear. Here, we investigated effects polyphenols from (pKAL) on HCT116 human colon cancer cells. pKAL induced cell death associated with alteration morphology DNA content. Annexin V PI staining revealed...
Western blot analysis of relative protein expression relies on appropriate reference proteins for data normalization. Small extracellular vesicles (sEVs), or exosomes, are increasingly recognized as potential indicators the physiological state cells due to their composition. Therefore, accurate sEVs quantification is crucial disease detection and prognosis applications. Currently, no documented ubiquitous identified precise normalization a interest in sEVs. Here we showed use total staining...
Artemisia annua L., (Gaddongsook in Korean), an annual herb, has been used for a long time Korean folk medicine the treatment of infectious or chronic disorder In addition, it possesses anti-inflammatory, and anti-cancer activity. However, molecular mechanism properties is still unclear. Here, we investigated effects polyphenols from (pKAL) on HCT116 human colon cancer cells. pKAL induced cell death associated with alteration morphology DNA content. Annexin V PI staining revealed that late...
Abstract Artemisia annua L., (Gaddongsook in Korean) has been used as Korean folk medicine for anti-inflammatory, and anticancer activities. However, the molecular mechanisms anti-cancer activities of L. still need elucidating. Here, we determine whether pKAL exhibit effects on RT-R breast cancer cells, characteristics radio-resistant (RT-R) cancer. In addition, further explore stemness epithelial-mesenchymal transition (EMT). MTT colony forming assay revealed that inhibited cell...