A5012633236- Multiple Sclerosis Research Studies
- Peripheral Neuropathies and Disorders
- Pluripotent Stem Cells Research
- Parkinson's Disease Mechanisms and Treatments
- Facial Nerve Paralysis Treatment and Research
- CRISPR and Genetic Engineering
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Alzheimer's disease research and treatments
- Autoimmune and Inflammatory Disorders Research
- Neurogenesis and neuroplasticity mechanisms
- Neurological disorders and treatments
- Systemic Lupus Erythematosus Research
- Polyomavirus and related diseases
- Protein Tyrosine Phosphatases
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Long-Term Effects of COVID-19
- Cancer Cells and Metastasis
- Ophthalmology and Eye Disorders
- Glioma Diagnosis and Treatment
- Sphingolipid Metabolism and Signaling
- Vaccine Coverage and Hesitancy
- Hormonal and reproductive studies
- Dermatological and COVID-19 studies
- Developmental Biology and Gene Regulation
Centro Hospitalar de Lisboa Ocidental
2014-2024
Universidade Nova de Lisboa
2013-2023
Hospital de Egas Moniz
2012-2023
Hospitais da Universidade de Coimbra
2023
University of Aveiro
2022
Rede de Química e Tecnologia
2022
University of Lisbon
2021
Centro Hospitalar Lisboa Norte
2020
Hospital de Santa Maria
2020
Université Laval
2010-2019
Abstract Human embryonic stem cells (hESCs) have been proposed as a source of dopamine (DA) neurons for transplantation in Parkinson's disease (PD). We investigated the effect vitro predifferentiation on vivo survival and differentiation hESCs implanted into 6-OHDA (6-hydroxydopamine)-lesion rat model PD. The were cocultured with PA6 16, 20, or 23 days, leading to DA neurons. Grafted hESC-derived survived well expressed neuronal markers. However, very few exhibited neuron phenotype. Reversal...
Abstract Glioblastoma multiforme (GBM) is a highly heterogeneous malignant tumor. Recent data suggests the presence of hierarchical organization within GBM cell population that involves cancer cells with stem-like behavior, capable repopulating tumor and contributing to its resistance therapy. Tumor stem are thought reside vascular niche provides structural functional support. However, most studies involve isolated grown under various culture conditions. Here, we use novel three-dimensional...
TAR DNA-binding protein 43 (TDP-43) is a major component in aggregates of ubiquitinated proteins amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Here we report that lipopolysaccharide (LPS)-induced inflammation can promote TDP-43 mislocalization aggregation. In culture, microglia astrocytes exhibited after exposure to LPS. Likewise, treatment the motoneuron-like NSC-34 cells with TNF-alpha (TNF-α) increased cytoplasmic levels TDP-43. addition, chronic...
Investigation in the field of Alzheimer´s disease (AD), commonest cause dementia, has been very active recent years and it may be difficult for clinician to keep up with all innovations aware implications they have clinical practice. The authors, thus, reviewed literature on theme order provide an updated overview, intended support decision-making aspects diagnosis management. This article begins focus concept AD its pathogenesis. Afterwards, epidemiology non-genetic risk factors are...
<b><i>Background:</i></b> Hormonal variations are known to influence the course of multiple sclerosis (MS). <b><i>Objectives:</i></b> We aimed evaluate impact menopause in MS course, including disease activity and disability progression. <b><i>Methods:</i></b> conducted a retrospective longitudinal cohort study all women, older than 44, post-menopausal, with diagnosis at least 1 year before menopause. evaluated comparing...
ORIGINAL RESEARCH article Front. Neuroanat., 30 December 2007 | https://doi.org/10.3389/neuro.05.004.2007
Alzheimer's disease (AD) occurs as either an autosomal dominant inherited or sporadically. While familial mutant genes can be expressed in cells animal models to assess dysregulated functions, sporadic AD cannot replicated given our lack of understanding causality. Fur thermore, the study forms is difficult inaccessibility brain tissues living individuals and manifestation symptoms years after onset disease. Here, objective was if induced pluripotent stem cell-derived neurons from...
BackgroundA significant proportion of pediatric-onset multiple sclerosis (POMS) patients do not respond to first-line disease-modifying therapies. Clinical trials showed that natalizumab is effective and safe in adults, but there are limited clinical trial data for children. Natalizumab currently prescribed off-label POMS. We aimed characterize the effectiveness, safety tolerability all POMS cases treated Portugal (from 2007 2018).MethodsData from records were retrospectively collected with...
Human stem cells are viewed as a possible source of neurons for cell-based therapy neurodegenerative disorders, such Parkinson's disease. Several protocols that generate different types from human (hSCs) have been developed. Nevertheless, the cellular mechanisms underlie development in vitro they subjected to specific differentiation often poorly understood. We designed focused DNA (oligonucleotide-based) large-scale microarray platform (named "NeuroStem Chip") and used it study gene...
Human embryonic stem cells (hESCs) are a potential source of dopaminergic neurons for treatment Parkinson's disease (PD). Dopaminergic can be derived from hESCs and display characteristic midbrain phenotype. Once transplanted, they induce partial behavioral recovery in animal models PD. The research field faces several challenges that need to overcome before clinical application transplantation therapy PD considered. These include low survival the hESC-derived, grafted after transplantation;...
Abstract The majority of human embryonic stem cell lines depend on a feeder layer for continuous growth in vitro, so that they can remain an undifferentiated state. Limited knowledge is available concerning the molecular mechanisms underlie capacity cells to support both proliferation and pluripotency these cells. Importantly, generally lose their vitro following long-term culture. In this study, we performed large-scale gene expression profiles foreskin fibroblasts during early,...
We performed a clinical report based, descriptive and retrospective study, aimed at comparing Flunarizine/Cinnarizine-induced parkinsonism (FCIP) patients Parkinson's disease (PD) patients. The FCIP group (n = 30) presented lower frequency of rigidity unilateral tremor than the PD 70). All improved, 13 after dopaminergic treatment. who improved spontaneously rigidity, compared with other subgroup group. did not improve showed pattern similar to
Multiple sclerosis is most often diagnosed among young adults but less frequently it may present during childhood or adolescence. In Portugal, there has been only one previous single-center, pediatric multiple study. The main objective was the evaluation of demographic, clinical, laboratorial and neuroradiological characteristics patients with pediatric-onset in Portugal. secondary objectives were to compare childhood-onset adolescent-onset characterize treatments prescribed.We performed a...
Fingolimod, an oral sphingosine 1-phosphate receptor modulator, is approved by EMA for relapsing-remitting multiple sclerosis (RRMS).To assess the effectiveness and safety of fingolimod in patients with RRMS real-world clinical practice Portugal.Retrospective, multicentre, non-interventional study, reporting 3 years follow-up data collected from October 2015 to July 2016. Sociodemographic previous treatments at baseline regarding disease evolution, including number relapses, annualised...