A5073817969- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- Vaccine Coverage and Hesitancy
- HIV Research and Treatment
- Virology and Viral Diseases
- COVID-19 and Mental Health
- Parasites and Host Interactions
- COVID-19 epidemiological studies
- Microbial Metabolic Engineering and Bioproduction
- Respiratory viral infections research
- Influenza Virus Research Studies
- Immune Cell Function and Interaction
- Escherichia coli research studies
- T-cell and B-cell Immunology
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Biochemical Acid Research Studies
- Bacterial Genetics and Biotechnology
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Invertebrate Immune Response Mechanisms
University of Maryland, Baltimore
2016-2025
University of Mary
2024
University of Baltimore
2023
Howard Hughes Medical Institute
2013-2016
F5 Networks (United States)
2015
National Institutes of Health
2014
Wittenberg University
2010
Ensco (United States)
2007
Purdue University West Lafayette
1992
Significance A highly effective malaria vaccine capable of long-term protection against genetically diverse strains is urgently needed. Here, we demonstrate that a three-dose regimen live attenuated whole-parasite conferred durable sterile through 33 weeks in ∼50% subjects controlled human infection strain heterologous to the strain. Prior studies by others and us have shown T cells are critical mediating after live-attenuated vaccination. provide evidence this Plasmodium falciparum...
The diversity of circulating human B cells is unknown. We use single-cell RNA sequencing (RNA-seq) to examine the both antigen-specific and total in healthy subjects malaria-exposed individuals. This reveals two cell lineages: a classical lineage activated resting memory an alternative lineage, which includes previously described atypical cells. Although have been associated with disease states, common controls, as well further track Plasmodium-specific after malaria vaccination naive...
<h3>Importance</h3> Human infections with the avian influenza A(H7N9) virus were first reported in China 2013 and continue to occur. Hemagglutinin H7 administered alone is a poor immunogen necessitating evaluation of adjuvanted H7N9 vaccines. <h3>Objective</h3> To evaluate immunogenicity safety an inactivated vaccine without AS03 adjuvant, as well mixed schedules that included sequential administration AS03- MF59-containing formulations unadjuvanted formulations. <h3>Design, Setting,...
Web-based survey data collection has become increasingly popular, and limitations on in-person during the COVID-19 pandemic have fueled this growth. However, anonymity of online environment increases risk fraudulent responses provided by bots or those who complete surveys to receive incentives, a major integrity. As part study return school, we implemented web-based parents in Maryland between December 2021 July 2022. Recruitment relied, part, social media advertisements. Despite...
γδ T cells are generally considered innate-like lymphocytes, however, an "adaptive-like" compartment has now emerged. To understand transcriptional regulation of adaptive cell immunobiology, we combined single-cell transcriptomics, receptor (TCR)-clonotype assignment, ATAC-seq, and immunophenotyping. We show that adult Vδ1+ segregate into TCF7+LEF1+Granzyme Bneg (Tnaive) or T-bet+Eomes+BLIMP-1+Granzyme B+ (Teffector) subtypes, with clonotypically expanded TCRs detected exclusively in...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTBiocatalytic synthesis of aromatics from D-glucose: the role transketolaseK. M. Draths, D. L. Pompliano, Conley, J. W. Frost, A. Berry, G. Disbrow, R. Staversky, and C. LievenseCite this: Am. Chem. Soc. 1992, 114, 10, 3956–3962Publication Date (Print):May 1, 1992Publication History Published online1 May 2002Published inissue 1 1992https://doi.org/10.1021/ja00036a050RIGHTS & PERMISSIONSArticle Views612Altmetric-Citations88LEARN ABOUT THESE...
Enteroaggregative Escherichia coli (EAEC) is a leading cause of acute and persistent diarrhea worldwide. A recently emerged Shiga-toxin-producing strain EAEC resulted in significant mortality morbidity due to progressive development hemolytic-uremic syndrome. The attachment the human intestinal mucosa mediated by aggregative adherence fimbria (AAF). Using X-ray crystallography NMR structures, we present new atomic resolution insight into structure AAF variant I from that caused deadly...
Malaria drives the adaptive differentiation of human γδ T cell repertoire.
Abstract Malaria vaccine development is hampered by extensive antigenic variation and complex life stages of Plasmodium species. Vaccine has focused on a small number antigens, many which were identified without utilizing systematic genome-level approaches. In this study, we implement machine learning-based reverse vaccinology approach to predict potential new malaria candidate antigens. We assemble analyze P. falciparum proteomic, structural, functional, immunological, genomic,...
Variant surface antigens (VSAs) play a critical role in severe malaria pathogenesis. Defining gaps, or "lacunae", immunity to these Plasmodium falciparum children with would improve our understanding of vulnerability and how protective develops. Using protein microarray 179 antigen variants from three VSA families as well more than 300 other blood stage P. antigens, reactivity was measured sera Malian cerebral malarial anaemia age-matched controls. Sera recognized fewer extracellular PfEMP1...
BACKGROUND AND OBJECTIVES: Although both licensed rotavirus vaccines are safe and effective, it is often not possible to complete the schedule by using same vaccine formulation. The goal of this study was investigate noninferiority immune responses 2 when administered as a mixed compared with administering single formulation alone. METHODS: Randomized, multicenter, open-label study. Healthy infants (6–14 weeks age) were randomized receive in 1 5 different schedules (2 for all doses, 3...
Abstract Vaccines based on Plasmodium falciparum apical membrane antigen 1 (AMA1) have failed due to extensive polymorphism in AMA1. To assess the strain-specificity of antibody responses malaria infection and AMA1 vaccination, we designed protein peptide microarrays representing hundreds unique variants. Following clinical episodes, children had short-lived, sequence-independent increases average whole-protein seroreactivity, as well strain-specific peptides diverse epitopes. Vaccination...
Abstract Background Safe and effective respiratory syncytial virus (RSV) vaccines remain elusive. This was a phase I/II trial (NCT02927873) of ChAd155-RSV, an investigational chimpanzee adenovirus-RSV vaccine expressing 3 proteins (fusion, nucleoprotein, M2-1), administered to 12–23-month-old RSV-seropositive children followed up for 2 years after vaccination. Methods Children were randomized receive doses ChAd155-RSV or placebo (at 1:1 ratio) (days 1 31). Doses escalated from 0.5 × 1010...
The World Health Organization (WHO) has called for new malaria vaccines with > 90% efficacy against Plasmodium falciparum infection to expand the anti-disease benefit provided by RTS,S/AS01 and R21/Matrix M subunit currently administered infants young children in sub-Saharan Africa. Attenuated P. sporozoites (PfSPZ) are being developed as a traveller's vaccine fulfill WHO's call high-level endemic countries support elimination. PfSPZ Vaccine, comprised of radiation-attenuated PfSPZ, was...
Nucleic acid testing (NAT) for malaria parasites is an increasingly recommended diagnostic endpoint in clinical trials of vaccine and drug candidates also important surveillance control elimination efforts. A variety reported NAT assays have been described, yet no formal external quality assurance (EQA) program provides validation the use. Here, we report results EQA exercise assays. Among five centers conducting controlled human infection trials, all achieved 100% specificity demonstrated...
Children 6 through 35 months of age are recommended to receive half the dose influenza vaccine compared with older children and adults. This was a 6-site, randomized 2:1, double-blind study comparing full-dose (0.5 mL) trivalent inactivated (TIV) half-dose (0.25 TIV in age. previously immunized (primed cohort) received 1 dose, those no previous immunizations (naive 2 doses TIV. Local systemic adverse events were recorded. Sera collected before immunization month after last Hemagglutination...
The malaria vaccine candidate RTS,S/AS01 is based on immunogenic regions of Plasmodium falciparum circumsporozoite protein (CSP) from the 3D7 reference strain and has shown modest efficacy against clinical disease in African children. It remains unclear what aspect(s) immune response elicited by this are protective. goals study were to measure diversity CSP, identify associations between polymorphism CSP risk P. infection disease. present includes data samples a prospective cohort designed...
Hemoglobin C trait, like hemoglobin S protects against severe malaria in children, but it is unclear whether trait also uncomplicated malaria. We hypothesized that Malian children with would have a lower risk of clinical than AA.Three hundred aged 0-6 years were enrolled cohort study incidence Bandiagara, Mali, continuous passive and monthly active follow-up from June 2009 to 2010.Compared AA (n = 242), AC 39) had longer time first episode (hazard ratio [HR], 0.19; P .001; 364 median...
Abstract Background A live-attenuated Plasmodium falciparum sporozoite (SPZ) vaccine (PfSPZ Vaccine) has shown up to 100% protection against controlled human malaria infection (CHMI) using homologous parasites (same P. strain as in the vaccine). Using a more stringent CHMI, with heterologous (different strain), we assessed impact of higher PfSPZ doses, novel multi-dose prime regimen, and delayed boost upon efficacy (VE). Methods We immunized 4 groups that each contained 15 healthy,...