A5078257061- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Glioma Diagnosis and Treatment
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- Cancer Genomics and Diagnostics
- Immune cells in cancer
- Wheat and Barley Genetics and Pathology
- Crop Yield and Soil Fertility
- Glycosylation and Glycoproteins Research
- Cancer Cells and Metastasis
- Epigenetics and DNA Methylation
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Mycotoxins in Agriculture and Food
- Plant Pathogens and Fungal Diseases
- Soybean genetics and cultivation
- Plant Stress Responses and Tolerance
- Muscle Physiology and Disorders
- Plant Disease Resistance and Genetics
- Genetic and phenotypic traits in livestock
- Circular RNAs in diseases
- Forest Insect Ecology and Management
- interferon and immune responses
- Plant Genetic and Mutation Studies
University of Calgary
2016-2025
University of Augsburg
2024-2025
Maize Research Institute Zemun Polje
2014-2025
Alberta Children's Hospital
2016-2024
Institute of Cancer Research
2022-2024
Cancer Institute (WIA)
2018-2021
Technical University of Munich
2021
Helmholtz Zentrum München
2019-2021
Klinikum rechts der Isar
2021
University of Modena and Reggio Emilia
2013-2020
Facioscapulohumeral muscular dystrophy has been genetically linked to reduced numbers (≤8) of D4Z4 repeats at 4q35 combined with 4A(159/161/168) DUX4 polyadenylation signal haplotype. However, we have recently reported that 1.3% healthy individuals carry this molecular signature and 19% subjects affected by facioscapulohumeral do not alleles eight or fewer repeats. Therefore, prognosis for carrying risk become more complicated. To test additional prognostic factors, measured the degree motor...
Chromatin accessibility discriminates stem from mature cell populations, enabling the identification of primitive stem-like cells in primary tumors, such as glioblastoma (GBM) where self-renewing driving cancer progression and recurrence are prime targets for therapeutic intervention. We show, using single-cell chromatin accessibility, that human GBMs harbor a heterogeneous population whose diversity is captured patient-derived (GSCs). In-depth characterization GSCs identifies three GSC...
Posterior fossa group A (PFA) ependymoma is a lethal brain cancer diagnosed in infants and young children. The lack of driver events the PFA linear genome led us to search its 3D for characteristic features. Here, we reconstructed genomes from diverse childhood tumor types uncovered global topology that highly reminiscent stem progenitor cells variety human tissues. remarkable feature exclusively present are type B ultra long-range interactions PFAs (TULIPs), regions separated by great...
Based on the 7-year experience of Italian Clinical Network for FSHD, we revised FSHD clinical form to describe, in a harmonized manner, phenotypic spectrum observed FSHD. The new Comprehensive Evaluation Form (CCEF) defines various categories by combination different features. inter-rater reproducibility CCEF was assessed between two examiners using kappa statistics evaluating 56 subjects carrying molecular marker used diagnosis. classifies: (1) presenting facial and scapular girdle muscle...
Objectives Facioscapulohumeral muscular dystrophy type 1 (FSHD1) has been genetically linked to reduced numbers (≤8) of D4Z4 repeats at 4q35. Particularly severe FSHD cases, characterised by an infantile onset and presence additional extra-muscular features, have associated with the shortest alleles 1–3 (1–3 DRA). We searched for signs perinatal evaluated disease outcome through systematic collection clinical anamnestic records de novo familial index cases their relatives, carrying DRA....
We investigated the role of 3D genome architecture in instructing functional properties glioblastoma stem cells (GSCs) by generating sub-5-kb resolution maps situ Hi-C. Contact at allow identification individual DNA loops, domain organization, and large-scale compartmentalization. observed differences looping architectures among GSCs from different patients, suggesting that is a further layer inter-patient heterogeneity for glioblastoma. Integration contact with chromatin transcriptional...
Inferring structural variants in cancer single-cell chromatin accessibility data links genetics to organization.
Changes in climate are likely to have a negative impact on water availability and soil fertility many maize-growing agricultural areas. The development of high-throughput phenotyping platforms provides new prospect for dissecting the dynamic complex plant traits such as abiotic stress tolerance into simple components. growth phenotypes 20 maize ( Zea mays L.) inbred lines were monitored non-invasive way under control, nitrogen, limitation well combined nitrogen using an automated system...
Glioblastomas (GBM) are aggressive brain tumors with extensive intratumoral heterogeneity that contributes to treatment resistance. Spatial characterization of GBMs could provide insights into the role tumor microenvironment in regulating heterogeneity. Here, we performed spatial transcriptomic and single-cell analyses mouse human GBM dissect impact distinct anatomical regions brains on GBM. In a syngeneic model, transcriptomics revealed numerous extracellular matrix (ECM) molecules,...
Pediatric glioblastoma (pGBM) is a lethal cancer with no effective therapies. To understand the mechanisms of tumor evolution in this cancer, we performed whole-genome sequencing linked reads on longitudinally resected pGBM samples. Our analyses showed that all diagnostic and recurrent samples were collections genetically diverse subclones. Clonal composition rapidly evolved at recurrence, less than 8% nonsynonymous single-nucleotide variants being shared diagnostic-recurrent pairs. track...
Lineage transformation between lung cancer subtypes is a poorly understood phenomenon associated with resistance to treatment and poor patient outcomes. Here, we aimed model this transition define underlying biological mechanisms identify potential avenues for therapeutic intervention. Small cell (SCLC) neuroendocrine in identity and, contrast non-SCLC (NSCLC), rarely contains mutations that drive the MAPK pathway. Likewise, NSCLCs transform SCLC concomitantly development of therapy...
Despite the addition of immune checkpoint blockade to first-line chemotherapy, prognosis for patients with small cell lung cancer (SCLC) is still devastating. For subset SCLC somatostatin receptor (SSTR) overexpression, radiopharmaceutical therapy (RPT) might be an effective future treatment option. <b>Methods:</b> Here, we present case a heavily pretreated stage IV patient showing exceptional response SSTR-directed RPT. A comprehensive translational work-up consisting histopathologic,...
Introduction Earlier sowing is a promising strategy of ensuring sufficiently high maize yields in the face negative environmental factors caused by climate change. However, it leads to low temperature exposure plants during emergence, warranting better understanding their response and acclimation suboptimal temperatures. Materials Methods To achieve this goal, whole transcriptome sequencing was performed on two inbred lines – tolerant/susceptible temperatures, at 5-day-old seedling stage....
Introduction: Glioblastoma or GBM is an aggressive brain cancer with a 5 year survival rate below 10 percent. Glioma stem cells GSCs drive formation, growth, and resistance. Previously, analysis of 17,601 phase contrast images from 15 patients revealed that neurodevelopmental form smaller, uniform clusters, while mesenchymal injury-response exhibit complex, irregular growth patterns. This new study examines the spatial distribution local cellular niche along gradient in primary GBM. We...
Maize is the most significant cereal which in Serbia last decade was harvested on approximately 1.15 million hectares with average yield 5300 kg ha−1 and total amount of production 5.7 tons (Đokovic et al. 2019). Aspergillus section Nigri poses challenges species classification due to similarity morphological chemical characteristics among them (Ismail, 2017). The first symptoms were observed maize September 2017, 2 3 weeks after silking form powdery or velvety black mycelium surface kernels...
IDH-wildtype glioblastoma (GBM) is an aggressive brain tumor with poor survival and few therapeutic options. Transcriptionally-defined cell states coexist in GBM occupy defined regions of the tumor. Evidence indicates that are plastic, but it remains unclear if they determined by underlying epigenetic state and/or microenvironmental factors. Here, we present spatially-resolved epigenomic profiling human tissues implicate chromatin structure as a key enabler plasticity. We report two...
Facioscapulohumeral muscular dystrophy (FSHD) is characterized by incomplete penetrance and intra-familial clinical variability. The disease has been associated with the genetic epigenetic features of D4Z4 repetitive elements at 4q35. Recently, hypomethylation proposed as a reliable marker in FSHD diagnosis. We exploited Italian Registry for FSHD, which families are classified using Clinical Comprehensive Evaluation Form (CCEF). A total 122 index cases showing classical phenotype (CCEF,...
Abstract Background In the context of early sowing maize as a promising adaptation strategy that could significantly reduce negative effects climate change, an in-depth understanding mechanisms underlying plant response to low-temperature stress is demanded. Although microRNAs (miRNAs) have been recognized key regulators response, research on their role in chilling tolerance during seedling stages scarce. Therefore, it great significance explore chilling-responsive miRNAs, reveal expression...
Restless legs syndrome is a frequent neurological disorder with substantial burden on individual well-being and public health. Genetic risk loci have been identified, but the causatives genes at these are largely unknown, so that functional investigation clinical translation of molecular research data still inhibited. To identify putatively causative genes, we searched for highly significant mutational in candidate genes.We analyzed 84 4,649 patients 4,982 controls by next generation...
Phenotypic measurements under controlled cultivation conditions are essential to gain a mechanistic understanding of plant responses environmental impacts and thus for knowledge-based improvement their performance natural field conditions. Twenty maize inbred lines (ILs) were phenotyped in response two levels water nitrogen supply (control stress) combined deficit. Over course 5 weeks (from about 4-leaf stage the beginning reproductive stage), phenology growth monitored by using...