A5100427428- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Acute Myeloid Leukemia Research
- MicroRNA in disease regulation
- Immune cells in cancer
- Developmental Biology and Gene Regulation
- Advanced Radiotherapy Techniques
- Receptor Mechanisms and Signaling
- Immune Response and Inflammation
- Renal and related cancers
- Wood and Agarwood Research
- Medical Imaging Techniques and Applications
- Hematopoietic Stem Cell Transplantation
- CRISPR and Genetic Engineering
- TGF-β signaling in diseases
- Cancer, Stress, Anesthesia, and Immune Response
- CAR-T cell therapy research
- Nonmelanoma Skin Cancer Studies
- High Altitude and Hypoxia
- FOXO transcription factor regulation
- Bone and Dental Protein Studies
- Lung Cancer Diagnosis and Treatment
- Circular RNAs in diseases
- IL-33, ST2, and ILC Pathways
Nanjing Medical University
2023-2024
Gansu University of Traditional Chinese Medicine
2024
Jiangsu Province Hospital
2023-2024
Nanjing General Hospital of Nanjing Military Command
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2023
State Administration of Traditional Chinese Medicine of the People's Republic of China
2023
Daping Hospital
2020-2023
Chongqing University
2023
Peking Union Medical College Hospital
2022-2023
Tsinghua University
2022-2023
Stromal cell–derived factor 1 (SDF-1) plays a major role in the migration, recruitment, and retention of endothelial progenitor cells to sites ischemic injury contributes neovascularization. We provide direct evidence demonstrating an important for heme oxygenase (HO-1) mediating proangiogenic effects SDF-1. Nanomolar concentrations SDF-1 induced HO-1 through protein kinase C ζ–dependent vascular growth factor–independent mechanism. SDF-1–induced tube formation migration was impaired...
Efficient transport of stem/progenitor cells without affecting their survival and function is a key factor in any practical cell-based therapy. However, the current approach using liquid nitrogen for transfer stem requires short delivery time window technically challenging financially expensive. The present study aims to use semipermeable alginate hydrogels (crosslinked by strontium) encapsulate, store, release cells, replace conventional cryopreservation method therapeutic within world-wide...
The development of human erythroid cells has been mostly examined in models adult hematopoiesis, while their early derivation during embryonic and fetal stages is largely unknown. We observed the maturation erythroblasts derived from pluripotent stem (hPSCs) by an efficient co-culture system. These hPSC-derived initially showed definitive characteristics with a glycophorin A+ (GPA+) CD34lowCD36− phenotype were distinct CD34+ cell-derived ones. After losing CD34 expression, GPA+CD36− matured...
Abstract Congenital scoliosis (CS) is a complex genetic disorder characterized by vertebral malformations. The precise etiology of CS not fully defined. Here, we identify that mutation in dual serine/threonine and tyrosine protein kinase ( dstyk ) lead to CS-like malformations zebrafish. We demonstrate the mutants related wavy malformed notochord sheath formation abnormal axial skeleton segmentation due dysregulated biogenesis vacuoles function. Further studies show DSTYK located late...
The mechanism by which Akt modulates stem cell homeostasis is still incompletely defined. Here we demonstrate that phosphorylates special AT-rich sequences binding protein 1 (SATB1) at serine 47 and protects SATB1 from apoptotic cleavage. Meanwhile, Oct4 threonine 228 Klf4 399, accelerates their degradation. Moreover, PI3K/Akt signaling enhances the of to Sox2, thereby probably impairing formation Oct4/Sox2 regulatory complexes. During retinoic acid (RA)-induced differentiation mouse F9...
Cefazolin sodium is an essential drug that widely used in clinical therapy for certain infective diseases caused by bacteria. As impurities are considered to be one of the most important causes safety issues, we studied embryotoxicity, cardiotoxicity, and neurotoxicity nine cefazolin zebrafish embryo larvae objective control impurity profiling. LC-MS/MS was employed analyze compound absorbance vivo, structure-toxicity relationship approached. Our results suggested structure MMTD...
Targeted therapy has a pivotal role for the treatment of liver cancer. The aim this current study was to examine effects decursin on growth HepG2 cells and underlying mechanisms. Our present showed that with significantly inhibited by suppressing cell proliferation, cycle arresting, promoting apoptosis in dose‐ time‐dependent manner. Most significantly, administration dramatically impeded vivo tumor nude mice. Mechanically, it is noteworthy provoked degradation YAP upregulating expression...
A Gram-staining-negative, aerobic, motile, oxidase- and catalase-positive, rod-shaped strain, designated ZS2-30(T), was isolated from Antarctic intertidal sandy sediment. The strain grew at 4-35 °C (optimum, 25 °C) in 0-25% (w/v) NaCl 3.0-4.0%). It could reduce nitrate to nitrite hydrolyse Tween 80. predominant cellular fatty acids of ZS2-30(T) were summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(16:0), C(18:1)ω9c, C(16:1)ω9c, C(12:0) 3-OH C(12:0). major polar lipids...
Impaired osteoblast differentiation may result in bone metabolic diseases such as osteoporosis. It was reported recently that hedgehog (Hh) signaling and autophagy are two important regulators of differentiation. In order to further dissect their relationship development, we used a zebrafish larvae model investigate how disruption one these signals affects the function other impacts Our results showed activation Hh negatively regulated autophagy. However, suppression by knocking down atg5...
Oral cancer, a predominant malignancy in developing nations, represents global health challenge with five-year survival rate below 50%. Nonetheless, substantial reductions both its incidence and mortality rates can be achieved through early detection appropriate treatment. Crucial to these treatment plans prognosis predictions is the identification of pathological type oral cancer.Toward this end, fiber-optic Raman spectroscopy emerges as an effective tool. This study combines technology...
Abstract Domestic animal embryonic stem (ES) cells would provide an invaluable research tool for genetic breeding and the production of transgenic animals. Unfortunately, authentic domestic animals ES have not been established despite progress made over more than two decades. Here, we show that ovine ES‐like can be efficiently derived propagated in a semi‐defined medium contains N2, B27, GSK3 inhibitor (CHIR99021), basic fibroblast growth factor (bFGF). These had characteristic...
Abstract Antagonism of ROS signaling can inhibit cell apoptosis and autophagy, thus favoring the maintenance expansion hematopoietic stem cells. Alpha lipoic acid (ALA), a small antioxidant molecule, affects by lowering level. In this study, we show that ALA promoted production human pluripotent cells (hPSCs) derived hemogenic endothelial stem/progenitor in vitro. Transcriptome analysis hPSCs showed endothelial-to-hematopoietic transition up-regulating RUNX1, GFI1, GFI1B, MEIS2, HIF1A...
Abstract Mast cells (MCs) play a pivotal role in the hypersensitivity reaction by regulating innate and adaptive immune responses. Humans have two types of MCs. The first type, termed MCTC, is found skin other connective tissues expresses both tryptase chymase, while second, MCT, which only tryptase, primarily mucosa. MCs induced from human adult-type CD34+ are reported to be MCT but development during embryonic/fetal stages largely unknown. Using an efficient coculture system, we identified...
Agarwood-a resinous wood produced by Aquilaria plants in response to injury or artificial induction-is a valuable medicinal and fragrance resource. Whole-Tree Agarwood-Inducing Technique (Agar-WIT) has been widely used produce agarwood. However, the time-dependent characteristics of agarwood formation induced Agar-WIT are yet be clarified. To promote technologically efficient utilization upgradation Agar-WIT, dynamic process mechanism were analyzed for one year.Agarwood percentage, barrier...
Abstract Type I IFN mediates the innate immune system to provide defense against viral infections. NF-κB–inducing kinase (NIK) potentiates basal activation of endogenous STING, which facilitates recruitment TBK1 with ectopically expressed IRF3 induce production. Moreover, NIK phosphorylates IKKα and confers its ability phosphorylate p100 (also known as NF-κB2) in mammals. Our study demonstrated that plays a critical role production teleost fish. It was found interacts cytoplasm at residue...
Jiahui Zeng, Huifang Zhang, Yuanling Liu, Wencui Sun, Danying Yi, Lijiao Zhu, Yonggang Xu Pan, Yijing Chen, Ya Zhou, Guohui Bian, Mowen Lai, Qiongxiu Jiaxin Bo Feng Ma. IJSC 2020;13:202-11. https://doi.org/10.15283/ijsc20033
The hematopoietic function of HOXC4 has not been extensively investigated. Our research indicated that induction in co-culture system from D10 significantly promoted productions most progenitor cells. CD34-CD43+ cells could be clearly classified into CD34-CD43low and CD34-CD43high sub-populations at D14. former had greater myelogenic potential, their production was influenced by HOXC4. By contrast, the latter potential to differentiate megakaryocytes erythroid cells, thus properties...
Tissue expansion is an invaluable reconstructive strategy after surgical excision of a congenital naevus. However, reducing the number operations very important for patients with giant We used large pedicle expanded flank flap to successfully treat circumferential naevus that extended from left upper arm wrist, which also provided excellent contour and colour match. consider this effective convenient method covering almost entire limb paediatric patient.
Seed cells and scaffold materials are essential components of tissue engineering. In this study, we investigated the key pathway zirconia/dental pulp stem cell composite in regulating macrophage polarization by transcriptome sequencing. We established N-rGO/ZrO2 confirmed its structure using various analytical techniques, including SEM, TEM, FTIR, Raman spectra, XPS, XRD. DPSCs were seeded onto material, their proliferation, adhesion, osteogenic differentiation evaluated CCK-8,...
Runt-related transcription factor 1 (RUNX1) is required for definitive hematopoiesis; however, the functions of most human RUNX1 isoforms are unclear. In particular, effects RUNX1-205 (a novel splice variant that lacks exon 6 in comparison with RUNX1b) on hematopoiesis not clear. this study, a embryonic stem cell (hESC) line inducible overexpression was established. Analyses these cells revealed induction at early stage did influence mesoderm but blocked emergence CD34+ cells, and production...