A5100683396- RNA modifications and cancer
- Cancer-related gene regulation
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Chronic Lymphocytic Leukemia Research
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- interferon and immune responses
- Epigenetics and DNA Methylation
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Adenosine and Purinergic Signaling
- Parkinson's Disease Mechanisms and Treatments
- Advanced Breast Cancer Therapies
- EEG and Brain-Computer Interfaces
- Diet, Metabolism, and Disease
- Immunodeficiency and Autoimmune Disorders
- Virus-based gene therapy research
- Sperm and Testicular Function
- Enzyme Production and Characterization
- Hippo pathway signaling and YAP/TAZ
Capital Medical University
2024-2025
Hubei University of Arts and Science
2025
Xiangyang Central Hospital
2025
Nanjing University of Chinese Medicine
2021-2025
Beijing Institute of Neurosurgery
2025
Beijing Anzhen Hospital
2025
Van Andel Institute
2024
Shanghai Jiao Tong University
2013-2024
Beijing Tian Tan Hospital
2024
University of Pennsylvania
2020-2024
• The lignin content of feedstock has been proposed as one key agronomic trait impacting biofuel production from lignocellulosic biomass. 4-Coumarate:coenzyme A ligase (4CL) is the enzymes involved in monolignol biosynthethic pathway. Two homologous 4CL genes, Pv4CL1 and Pv4CL2, were identified switchgrass (Panicum virgatum) through phylogenetic analysis. Gene expression patterns enzymatic activity assays suggested that biosynthesis. Stable transgenic plants obtained with down-regulated. RNA...
High-risk human papillomavirus (HR-HPV) has been recognized as a major causative agent for cervical cancer. Upon HPV infection, early genes E6 and E7 play important roles in maintaining malignant phenotype of cancer cells. By using clustered regularly interspaced short palindromic repeats- (CRISPR-) associated protein system (CRISPR/Cas system), widely used genome editing tool many organisms, to target HPV16-E7 DNA positive cell lines, we showed the first time that single-guide RNA (sgRNA)...
Abstract The pathological role of reactive gliosis in CNS repair remains controversial. In this study, using murine ischemic and hemorrhagic stroke models, we demonstrated that microglia/macrophages astrocytes are differentially involved engulfing synapses the region. By specifically deleting MEGF10 MERTK phagocytic receptors, determined inhibiting phagocytosis or improved neurobehavioral outcomes attenuated brain damage. stroke, but not outcomes. Single-cell RNA sequencing revealed related...
Abstract Objective Aimed to evaluate the long‐term clinical and radiographic outcomes of short implants supporting single crowns in posterior regions. Material methods A retrospective study design was adopted. The data 231 (intra‐bony length ≤8 mm) 168 patients, were collected after 5–10 (mean 7.22) years' follow‐up. Implant prosthesis failures, peri‐implant marginal bone loss, biological technical complications evaluated. influence factors on implant failure studied. Results In total 4 11...
Abstract Background CircRNAs are found to affect initiation and progression of several cancer types. However, whether circRNAs implicated in gallbladder (GBC) remains obscure. Methods We perform RNA sequencing 10 pairs GBC para-cancer tissues. CCK8 clone formation assays used evaluate proliferation ability cells. qPCR Western blot determine expression RNAs proteins, respectively. CircRNA-protein interaction is confirmed by pulldown, immunoprecipitation, fluorescence situ hybridization....
BackgroundPancreatic cancer (PC) is one of the most lethal solid malignancies in world due to its excessive cell proliferation and aggressive metastatic features. Emerging evidences revealed importance posttranscriptional modifications RNAs PC progression. However, knowledge about 5-methylcytosine (m5C) RNA modification still extremely limited. In this study, we attempted explore expression changes clinical significances 12 known m5C-related genes among patients.MethodsA total 362 normal 382...
• The down-regulation of enzymes the monolignol pathway results in reduced recalcitrance biomass for lignocellulosic ethanol production. Cinnamoyl CoA reductase (CCR) catalyzes first step phenylpropanoid specifically dedicated to biosynthesis. However, plants contain multiple CCR-like genes, complicating selection lignin-specific targets. This study was undertaken understand complexity CCR gene family tetraploid switchgrass (Panicum virgatum) and determine biochemical properties encoded...
Abstract Gemcitabine is the first-line treatment for locally advanced and metastatic gallbladder cancer (GBC), but poor gemcitabine response universal. Here, we utilize a genome-wide CRISPR screen to identify that loss of ELP5 reduces gemcitabine-induced apoptosis in GBC cells P53-dependent manner through Elongator complex other uridine 34 (U ) tRNA-modifying enzymes. Mechanistically, impairs integrity stability abrogate wobble U tRNA modification, directly impedes modification-dependent...
Oxidation of 5-methylcytosine (5mC) in DNA by the ten-eleven translocation (TET) family enzymes is indispensable for gene regulation mammals. More recently, evidence has emerged to support a biological function TET-mediated m5C oxidation messenger RNA. Here, we describe previously uncharacterized role transfer RNA (tRNA). We found that product 5-hydroxylmethylcytosine (hm5C) specifically enriched tRNA inside cells and activity TET2 on tRNAs can be readily observed vitro. further hm5C levels...
The posttranscriptional modification of messenger RNA (mRNA) and transfer (tRNA) provides an additional layer regulatory complexity during gene expression. Here, we show that a tRNA methyltransferase, TRMT10A, interacts with mRNA demethylase FTO (ALKBH9), both in vitro inside cells. TRMT10A installs N 1 -methylguanosine (m G) tRNA, performs demethylation on 6 -methyladenosine A) ,2′- O -dimethyladenosine A m ) mRNA. We ablation not only leads to decreased G but also significantly increases...
Understanding the burdens and trends of non-alcoholic fatty liver disease (NAFLD) is necessary for developing effective intervention strategies. In this study, Global Burden Disease (GBD) 2019 study data were extracted analyzed to elucidate NAFLD.The prevalence, incidence, disability-adjusted life year (DALY), death rates NAFLD in geographic populations worldwide from 1990 GBD data. The global temporal trend was evaluated using estimated annual percentage changes (EAPCs) age-standardized...
Abstract N 6 -methyladenosine (m A), the most prevalent internal modification on eukaryotic mRNA, plays an essential role in various stress responses. The brain is uniquely vulnerable to cellular stress, thus defining how m A sculpts brain’s susceptibility may provide insight aging and disease-related stress. Here we investigate impact of mRNA methylation adult Drosophila with We show that enriched increases heat Through A-immunoprecipitation sequencing, 5′UTR Mettl3 -dependent transcripts...
Abstract Murine Aim2 and p202 proteins (encoded by the Ifi202 genes) are members of IFN-inducible p200 protein family. Both can sense dsDNA in cytoplasm. However, upon sensing dsDNA, only through its pyrin domain form an inflammasome to activate caspase-1 induce cell death. Given that has been predicted inhibit activation increased levels female mice certain strains associated with lupus susceptibility, we compared expression genes between Aim2-deficient age-matched wild-type mice. We found...
Among 367 subjects, the authors analyzed 167 patients with essential tremor, sporadic progressive cerebellar ataxia, multiple-system atrophy, and atypical parkinsonism 200 healthy control subjects for FMR1 premutation alleles. None of carried alleles within range. These findings suggest that in absence other supportive clinical or imaging features, cost-effectiveness routine fragile X tremor/ataxia syndrome screening this Asian cohort movement disorders was low.
// Jun-Jie Chen 1,* , H.-C. Jennifer Shen Leslie A. Rivera Rosado 1 Yaqin Zhang Xu Di and Baolin Division of Therapeutic Proteins, Office Biotechnology Products, Center for Drug Evaluation Research, Food Administration, Bethesda, Maryland, United States * Denotes equal contribution Correspondence: Zhang, email: Keywords : death receptors, cellular localization, targeted cancer therapy, drug resistance Received July 09, 2012, Accepted August 18, Published 19, 2012 Abstract Multiple clinical...
Abstract Gallbladder cancer (GBC) is a malignant with very poor prognosis. Although promyelocytic leukemia zinc-finger protein (PLZF) was reported to be deregulated in numerous cancers and also relevant clinical prognosis, its role GBC progression has been little known. In this study, we found PLZF expression decreased GBC, correlating advanced TNM stage, distant metastasis, shorter overall survival. Moreover, ectopic cells (NOZ GBC-SD) significantly reduced the cell proliferation,...
N6 -methyladenosine (m 6 A) is the most abundant modification on messenger RNAs (mRNAs) and catalyzed by methyltransferase-like protein 3 (Mettl3). To understand role of m A in a self-renewing somatic tissue, we deleted Mettl3 epidermal progenitors vivo. Mice lacking demonstrate marked features dysfunctional development self-renewal, including loss hair follicle morphogenesis impaired cell adhesion polarity associated with oral ulcerations. We show that promotes A-mediated degradation mRNAs...
Background: To analyze causality between gut microbiota and chronic spontaneous urticaria (CSU) to investigate the mediating effect of metabolic pathways. Methods: We extracted genome-wide association study summary statistics for 211 taxa from MiBioGen consortium (N=18 340), 205 pathways Dutch Microbiome Project (N=7738), CSU FinnGen genomics initiative (N=450). Bidirectional Mendelian randomization (MR) was performed detect genetic microbiota, bacterial pathways, CSU. Sensitivity analyses...
BACKGROUND Gastric neuroendocrine carcinomas (NECs) exhibit aggressive features, such as rapid growth, higher rate of metastasis, and a generally unfavorable prognosis compared to gastric adenocarcinoma. As result, therapeutic options for NECs remain limited, contributing the poor patients. Immunotherapy has emerged promising treatment strategy demonstrated potential partially improve survival patients with NECs. Nevertheless, unique clinical response termed pseudoprogression (PsP) garnered...