A5054244975- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Phytochemicals and Antioxidant Activities
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Metabolomics and Mass Spectrometry Studies
- Polymer crystallization and properties
- Immune Cell Function and Interaction
- Analytical chemistry methods development
- Diet, Metabolism, and Disease
- Block Copolymer Self-Assembly
- Metabolism, Diabetes, and Cancer
- RNA and protein synthesis mechanisms
- Polymer Nanocomposites and Properties
- Ubiquitin and proteasome pathways
- Aquaculture disease management and microbiota
- Cellular transport and secretion
- Cancer Immunotherapy and Biomarkers
- Hepatitis C virus research
- Cancer, Hypoxia, and Metabolism
- Cultural Heritage Materials Analysis
- Microbial Metabolites in Food Biotechnology
- vaccines and immunoinformatics approaches
- Plant Stress Responses and Tolerance
- biodegradable polymer synthesis and properties
Second Hospital of Anhui Medical University
2025
Anhui Medical University
2025
Pfizer (United States)
2019-2025
Ningbo University
2021-2024
Hebei University
2023
Zhejiang University
2004-2022
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2022
Abbott (United Kingdom)
2022
First Affiliated Hospital Zhejiang University
2022
University of Massachusetts Dartmouth
2020-2022
Sucrose nonfermenting 1 (SNF1)-related protein kinase 2s (SnRK2s) are central components of abscisic acid (ABA) signaling pathways. The snrk2.2/2.3/2.6 triple-mutant plants nearly completely insensitive to ABA, suggesting that most the molecular actions ABA triggered by SnRK2s-mediated phosphorylation substrate proteins. Only a few proteins SnRK2s known. To identify additional and provide insight into we used quantitative phosphoproteomics compare global changes in phosphopeptides WT triple...
Significance Protein phosphorylation is a major regulatory mechanism for many cellular functions, but no phosphoprotein in biofluids has been developed disease diagnosis because of the presence active phosphatases. This study presents general strategy to isolate and identify phosphoproteins extracellular vesicles (EVs) from human plasma as potential markers differentiate healthy states. We identified close 10,000 unique phosphopeptides EVs small volumes samples more than 100 that are...
Mutations in <i>PINK1</i> (PTEN-induced putative kinase 1) cause early onset familial Parkinson's disease (PD). PINK1 accumulates on the outer membrane of damaged mitochondria followed by recruiting parkin to promote mitophagy. Here, we demonstrate that BCL2/adenovirus E1B 19-kDa interacting protein 3 (BNIP3), a mitochondrial BH3-only protein, interacts with accumulation full-length mitochondria, which facilitates recruitment and PINK1/parkin-mediated Inactivation BNIP3 mammalian cells...
Abscisic acid (ABA) is an important phytohormone controlling responses to abiotic stresses and sensed by proteins from the PYR/PYL/RCAR family. To explore genetic contribution of PYLs toward ABA-dependent ABA-independent processes, we generated characterized high-order Arabidopsis mutants with mutations in PYL We obtained a pyl quattuordecuple mutant found that it was severely impaired growth failed produce seeds. Thus, carried out detailed characterization duodecuple mutant,...
PF-06651600 was developed as an irreversible inhibitor of JAK3 with selectivity over the other three JAK isoforms. A high level toward is achieved by covalent interaction a unique cysteine residue (Cys-909) in catalytic domain JAK3, which replaced serine Importantly, 10 kinases kinome have at equivalent position Cys-909 JAK3. Five those belong to TEC kinase family including BTK, BMX, ITK, RLK, and are also inhibited PF-06651600. Preclinical data demonstrate that inhibition cytolytic function...
Our understanding of the molecular control many disease pathologies requires identification direct substrates targeted by specific protein kinases. Here we describe an integrated proteomic strategy, termed kinase assay linked with phosphoproteomics, which combines a sensitive reaction endogenous kinase-dependent phosphoproteomics to identify The unique in vitro is carried out highly efficient manner using pool peptides derived directly from cellular and then dephosphorylated as substrate...
Abstract Background Epstein-Barr virus (EBV) is an oncovirus belonging to the herpesvirus family, associated with pathogenesis of multiple malignancies, particularly Burkitt lymphoma (BL). The remains latent in host cells and plays a critical role tumor progression through various mechanisms. A key glycoprotein, gp350, expressed during lytic phase EBV, instrumental viral entry into B presents unique antigenic target, making it promising candidate for immunotherapeutic approaches, such as...
Global phosphorylation changes in plants response to environmental stress have been relatively poorly characterized date. Here we introduce a novel mass spectrometry-based label-free quantitation method that facilitates systematic profiling plant phosphoproteome with high efficiency and accuracy. This employs synthetic peptide libraries tailored specifically as internal standards for complex phosphopeptide samples accordingly, local normalization algorithm, LAXIC, which calculates abundance...
Cranberries contain various constituents relevant to human health. Our previous study demonstrated the chemopreventive effects of whole cranberry against colon cancer in mice. In order determine role different secondary metabolites inhibiting cancer, ethyl acetate extract (EAE) and polyphenol (PPE) were obtained. The free-radical scavenging activities chemical composition extracts determined. EAE consisted triterpenes sterols a trace amount proanthocyanidins. PPE mainly contained with...
Branched chain amino acid (BCAA) catabolic impairments have been implicated in several diseases. ketoacid dehydrogenase (BCKDH) controls the rate limiting step BCAA degradation, activity of which is inhibited by BCKDH kinase (BDK)-mediated phosphorylation. Screening efforts to discover BDK inhibitors led identification thiophene PF-07208254, improved cardiometabolic endpoints mice. Structure-activity relationship studies a thiazole series inhibitors; however, these did not improve metabolism...
Elucidating how serine/threonine phosphatases regulate kinase function and bacterial virulence is critical for our ability to combat these infections. Group B streptococci (GBS) are β-hemolytic Gram-positive bacteria that cause invasive infections in humans. To adapt environmental changes, GBS encodes signaling mechanisms comprising two component systems eukaryotic-like enzymes. We have previously described the importance of Stk1 pathogenesis. However, presence or absence cognate phosphatase...
Protein quality control (PQC) plays an important role in stemming neurodegenerative diseases and is essential for the growth of some cancers. Valosin-containing protein (VCP)/p97 a pivotal multiple PQC pathways by interacting with numerous adaptors that link VCP to specific substrates influence post-translational modification state substrates. However, our poor understanding specificity architecture adaptors, dynamic properties their interactions hinders fundamental features how modulation...
Kinase mediated phosphorylation signaling is extensively involved in cellular functions and human diseases, unraveling networks requires the identification of substrates targeted by kinases, which has remained challenging. We report here a novel proteomic strategy to identify specificity direct kinases coupling phosphoproteomics with sensitive stable isotope labeled kinase reaction. A whole cell extract was moderately dephosphorylated subjected vitro reaction under condition (18)O-ATP...
S. flexneri is a widespread foodborne pathogen causing food contamination and responsible for poisoning outbreaks related to various foods in developing countries. Not only has biofilm formation by been difficult eliminate, but it also increased the drug resistance of strain. In present study, was demonstrated that L-EPSs secreted Lactobacillus plantrum 12 could inhibit on, adhesion to, invasion into HT-29 cells. Also, decrease minimum elimination concentration (MBEC) antibiotics used...
Direct analysis in real time (DART) mass spectrometry is a recently developed innovative technology, which has shown broad applications for fast and convenient of complex samples. Due to the ease sample preparation, we have initiated an investigation feasibility detecting nucleotides nucleosides using DART-AccuTOF instrument, will refer as DART spectrometer. Our experimental results reveal that ions representing intact molecules are not detectable either positive-ion or negative-ion mode....
Northern snakehead (Channa argus) is an indigenous fish species and one of popularly cultured snakeheads in China other Asian countries. Unfortunately, Nocardia seriolae infections have caused considerable losses the aquaculture industry. However, infectivity immune response induced by N. are unclear. In order to better understand a series time points after challenge, we conducted transcriptomic comparison spleen at 48, 96, 144 h challenge seriola against their control counterparts. Gene...
Acute Myeloid Leukemia (AML) is a prevalent neoplastic disorder. The roles of E3 ubiquitin ligases and related genes in AML drug resistance prognosis remain unclear. Genes were identified from GeneCards UniProt databases, differentially expressed selected based on transcriptional sequencing data wild-type Adriamycin-resistant HL60 (HL60/WT & HL60/ADR) cell lines, the intersection these three sources was taken. We then constructed prognostic model comprising five (HBP1, RNF130, RMND5B,...
Bottom-up mass spectrometry-based proteomics studies changes in protein abundance and structure across conditions. Since the currency of these experiments are peptides, i.e. subsets sequences that carry quantitative information, conclusions at a different level must be computationally inferred. The inference is particularly challenging situations where peptides shared by multiple proteins or post-translational modifications. While many approaches infer underlying abundances from unique there...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStudies of the mechanism .DELTA.5-3-ketosteroid isomerase reaction by substrate, solvent, and combined kinetic deuterium isotope effects on wild-type mutant enzymesLiang Xue, Paul Talalay, Albert S. MildvanCite this: Biochemistry 1990, 29, 32, 7491–7500Publication Date (Print):August 14, 1990Publication History Published online1 May 2002Published inissue 14 August...
Protein kinases are implicated in multiple diseases such as cancer, diabetes, cardiovascular diseases, and central nervous system disorders. Identification of kinase substrates is critical to dissecting signaling pathways understanding disease pathologies. However, methods techniques used identify bona fide have remained elusive. Here we describe a proteomic strategy suitable for identifying specificity direct high throughput. This approach includes an vitro assay-based substrate screening...
Two efficient transition-metal-free highly regioselective pathways for constructing sulfonylated imidazoles via three-component reactions of amidines, ynals, and sodium sulfonates have been developed. The generations different were simply controlled by additives. In addition, this method features environmental friendliness, good functional group tolerance, high atom economy, which makes it practical.
ABSTRACT Regulation of glucose transport, which is central for control whole-body metabolism, determined by the amount GLUT4 transporter (also known as SLC2A4) in plasma membrane (PM) fat and muscle cells. Physiologic signals [such activated insulin receptor or AMP-activated protein kinase (AMPK)] increase PM GLUT4. Here, we show that distribution between interior human cells dynamically maintained, AMPK promotes redistribution regulating exocytosis endocytosis. Stimulation mediated Rab10...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStereochemistry of the concerted enolization catalyzed by .DELTA.5-3-ketosteroid isomeraseA. Kuliopulos, G. P. Mullen, L. Xue, and Albert S. MildvanCite this: Biochemistry 1991, 30, 13, 3169–3178Publication Date (Print):April 2, 1991Publication History Published online1 May 2002Published inissue 2 April 1991https://pubs.acs.org/doi/10.1021/bi00227a003https://doi.org/10.1021/bi00227a003research-articleACS PublicationsRequest reuse permissionsArticle...