A5101672257- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- Monoclonal and Polyclonal Antibodies Research
- Metastasis and carcinoma case studies
- Epigenetics and DNA Methylation
- Esophageal Cancer Research and Treatment
- RNA Research and Splicing
- Gut microbiota and health
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- Inflammatory Biomarkers in Disease Prognosis
- Genetic factors in colorectal cancer
- Redox biology and oxidative stress
- Fibroblast Growth Factor Research
- Protein Tyrosine Phosphatases
- Ferroptosis and cancer prognosis
- Gastric Cancer Management and Outcomes
- Advanced Biosensing Techniques and Applications
- Traditional Chinese Medicine Analysis
- Yersinia bacterium, plague, ectoparasites research
- Adipose Tissue and Metabolism
- Extracellular vesicles in disease
- Circular RNAs in diseases
Jianghan University
2024
First Affiliated Hospital of Xi'an Jiaotong University
2016-2024
Wenzhou Medical University
2014-2024
Hangzhou Medical College
2024
Zhejiang Provincial People's Hospital
2024
First Affiliated Hospital of Wenzhou Medical University
2015-2024
Zhejiang Academy of Medical Sciences
2020-2023
Nanjing Medical University
2017-2023
Jiangsu Province Hospital
2023
Guangdong Pharmaceutical University
2020
Abstract DNA polymerase ζ participates in translesional bypass replication. Here we show that reduced expression of the catalytic subunit hREV3 renders human fibroblasts more sensitive to cytotoxic effect cisplatin, reduces their sensitivity ability cisplatin exposure generate drug resistant variants surviving population, and rate emergence resistance at population level. Reduction REV3 mRNA did not alter adduct removal but impair both spontaneous cisplatin-induced extrachromosomal...
Pancreatic cancer (PC) is one of the most lethal malignances. Identification biomarkers for early diagnosis PC a key imperative. MicroRNAs (miRNAs) have been shown to be valuable in context several cancers. Exosomes refer vesicles released by tumor cells at stage disease. Thus, detection miRNA exosomes can used as potential biomarker PC. In this study, we profiled serum levels miRNAs patients with chronic pancreatitis (CP) and PC; role miR‑23b‑3p progression was assessed vitro. Additionally,...
Colorectal cancer (CRC) is the second common cause of cancer-related human mortalities. Dysregulation histone 3 (H3) methylation could lead to transcriptional activation multiple oncogenes, which closely associated with CRC tumorigenesis and progression. Nuclear receptor-binding SET Domain protein 2 (NSD2) a key methyltransferase catalyzing H3 lysine 36 dimethylation (H3K36me2). Its expression, potential functions, molecular mechanisms in are studied here. Gene Expression Profiling...
Ninjurin 2 (NINJ2) is a novel adhesion molecule. Its expression and potential function in human colorectal cancer (CRC) cells are studied. We show that NINJ2 overexpressed established (HT-29) primary CRC colon tissues. level low epithelial normal shRNA or knockout (by CRSIPR/Cas9) potently inhibited cell survival proliferation, while significantly inducing apoptosis. Conversely, lentivirus-mediated overexpression promoted proliferation. co-immunoprecipitated with multiple RTKs (EGFR,...
Backgrounds and aims Immunotherapies have formed an entirely new treatment paradigm for hepatocellular carcinoma (HCC). Tertiary lymphoid structure (TLS) has been associated with good response to immunotherapy in most solid tumors. Nonetheless, the role of TLS human HCC remains controversial, recent studies suggest that their functional heterogeneity may relate different locations within tumor. Exploring factors influence formation provide more useful insights. However, affecting presence...
Background Several genetic variants including PSCA rs2294008 C>T and rs2976392 G>A, MUC1 rs4072037 T>C, PLCE1 rs2274223 A>G have shown significant association with stomach cancer risk in the previous genome-wide studies (GWASs). Methods To evaluate associations of these SNPs Han Chinese, an independent hospital based case-control study was performed by genotyping four polymorphisms a total 692 cases 774 healthy controls acquired using frequency matching for age gender. False-positive report...
Treatment and relevant targets for breast cancer (BC) remain limited, especially triple-negative BC (TNBC). We identified 6091 proteins of 76 human cell lines using data-independent acquisition (DIA). Integrating our proteomic findings with prior multi-omics datasets, we found that including proteomics data improved drug sensitivity predictions provided insights into the mechanisms action. subsequently profiled changes in nine (five TNBC four non-TNBC) treated EGFR/AKT/mTOR inhibitors. In...
Purpose Research on bone metastasis in esophageal cancer (EC) is relatively limited. Once occurs patients, their prognosis poor, and it severely affects quality of life. Currently, there a lack convenient tumor markers for early identification EC. Our research aims to explore whether neutrophil-lymphocyte ratio (NLR) can predict patients with Methods Retrospective analysis clinical indicators was performed 604 They were divided into groups based or not metastasis, the patients’...
Objective This study aims to elucidate the antioxidative mechanisms of tanshinone IIA (Tan IIA) in ameliorating hydrogen peroxide (H2O2)-induced oxidative stress Huh7 cellular model. Methods The association between Tan and HCC was analyzed using network pharmacology techniques. Changes activity on H2O2-induced cells related antioxidant indices were determined CCK-8. underwent analysis Western blot immunofluorescence assessments delineate concentrations PI3K/Akt Nrf2/HO-1 proteins their...
The oncogenic fusion protein nucleophosmin-anaplastic lymphoma kinase (NPM-ALK), found exclusively in a subset of ALK-positive anaplastic large cell lymphoma, promotes tumorigenesis by exerting its constitutively active tyrosine activity. Thus, characterization the NPM-ALK-induced changes phosphoproteome will likely provide insights into biology this oncoprotein. To achieve goal, we used strategy combining sequential affinity purification phosphopeptides and LC/MS. GP293 cells transfected...
Background: Cancer is a leading cause of death worldwide. Extensive research over decades has led to the development therapies that inhibit oncogenic signaling pathways. The mammalian target rapamycin (mTOR) pathway plays an important role in many cancers. Several mTOR inhibitors are approved for treatment However, anticancer efficacies inhibitor monotherapy still limited. Methods: Western blot was used detect expression indicated molecules. Thioredoxin reductase (TrxR) activity cells...
Colon cancer is one of the leading causes cancer-related death in world. The development new drugs and therapeutic strategies for patients with colon are urgently needed. Isodeoxyelephantopin (ESI), a sesquiterpene lactone isolated from medicinal plant Elephantopus scaber L., has been reported to exert antitumor effects on several cells. However, molecular mechanisms underlying action ESI still elusive. In present study, we found that potently suppressed cell proliferation human Furthermore,...
Previous studies have shown that the kinase activation loop (KAL) of oncogenic fusion protein NPM-ALK regulates its overall tyrosine phosphorylation status and tumorigenicity. Using tandem affinity purification-mass spectrometry, we assessed how KAL individual tyrosines. lysates GP293 cells transfected with NPM-ALK, our highly reproducible results showed evidence in all 3 tyrosines 8 outside KAL. We created 7 mutants, each which carried a Tyr-to-Phe mutation >or=1 A complete loss...
Background P120 catenin (p120ctn), a regulator of cell adhesion, has previously been found in many malignancies, and suggested role invasion, metastasis survival. The aim this study was to investigate correlations between altered localization p120ctn clinical-pathological characteristics esophageal squamous carcinoma (ESCC). Methods Immunohistochemical staining for performed on tissue samples from 118 patients with ESCC. expression scored intensity cellular by Image-pro Plus 6.0....
The aim of this study was to discuss the role c-KIT mutation in pathogenesis gastrointestinal stromal tumors (GISTs) and analyze its correlation with proliferation apoptosis. PDGFRA genotypes were examined by deoxyribonucleic acid sequencing. Immunohistochemistry performed determine expression levels Kit, Ki-67 (proliferation marker), apoptotic protease-activating factor (APAF)-1 (apoptosis marker) relationship between their three genes. In 68 cases examined, 44 (64.7%) showed mutations one...
Gene therapy is one of the most promising potential therapeutic strategies for many types cancer. Cell apoptosis an active, programmed physiological process body, and its disruption has been closely associated with occurrence tumor development. Apoptin known to induce cell apoptosis. In present study, MCF-7 breast cancer line was transfected a human serum albumin (HSA) apoptin expressing plasmid [HSA‑polyethylenimine (PEI)-pcDNA-Apoptin]. Reverse transcription‑quantitative polymerase chain...
Comprehensive proteome profiling of breast cancer tissue samples is challenging, as the contain many proteins with varying concentrations and modifications. We report an effective sample preparation strategy combined liquid chromatography (LC) electrospray ionization (ESI) quadrupole time-of-flight (QTOF) MS/MS for analysis human tissue. The complexity was reduced by using protein precipitation from a extract, followed sequential solubilization in solvents different solubilizing strength....
PBX3 (Pre-B-cell leukemia homeobox 3) had been considered to be a multifunctional oncogene which involved in tumor growth, invasion, and metastasis some solid tumors. However, the contribution of papillary thyroid carcinoma (PTC) remains unclear. In this study, we found that expression was significantly upregulated PTC tissues compared adjacent normal tissues, high levels were correlated with size, lymphatic metastasis, TMN stage, poor prognosis patients. Overexpression cell lines promoted...
Aims Interventions using prebiotic inulin-type fructans (ITFs) are widely prescribed to modulate the gut microbiota composition and activity promote health. However, impacts of ITFs on post-antibiotic reconstitution microbiome remain incompletely understood. The aim present study was investigate effects supplementation intestinal inflammation, colonic transcriptome after antibiotic treatment. Methods Results Male BALB/c mice were subjected an cocktail (ABx) treatment for 7 days, their...