A5012181891- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Electrospun Nanofibers in Biomedical Applications
- Electrohydrodynamics and Fluid Dynamics
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Advanced oxidation water treatment
- Anatomy and Medical Technology
- Cancer Cells and Metastasis
- Ferroptosis and cancer prognosis
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Advanced Photocatalysis Techniques
- Planarian Biology and Electrostimulation
- Periodontal Regeneration and Treatments
- Electrochemical Analysis and Applications
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Electrocatalysts for Energy Conversion
- Cellular Mechanics and Interactions
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- interferon and immune responses
Zhejiang University
2018-2025
First Affiliated Hospital Zhejiang University
2025
Zhengzhou University
2019-2024
Shandong University of Traditional Chinese Medicine
2024
Hangzhou Medical College
2022-2024
Zhejiang Provincial People's Hospital
2022-2024
Zhejiang Cancer Hospital
2023-2024
Kunming University of Science and Technology
2024
Southwest Medical University
2015-2023
Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine
2018-2022
Gelatin methacryloyl (GelMA), a photocurable hydrogel, is widely used in 3D culture, particularly bioprinting, due to its high biocompatibility, tunable physicochemical properties, and excellent formability. However, as the properties performances of GelMA vary under different synthetic conditions, there lack standardization, leading conflicting results. In this study, uniform standard established understand enhance applications. First, basic concept density molecular network (DMN) are...
Abstract In-situ bioprinting is attractive for directly depositing the therapy bioink at defective organs to repair them, especially occupations such as soldiers, athletes, and drivers who can be injured in emergency. However, traditional displays obvious limitations its complex operation environments. Here, we design a bioconcrete with electrosprayed cell-laden microgels aggregate gelatin methacryloyl (GelMA) precursor solution cement. Promising printability guaranteed wide temperature...
Abstract Low‐concentration gelatin methacryloyl (GelMA) has excellent biocompatibility to cell‐laden structures. However, it is still a big challenge stably fabricate organoids (even microdroplets) using this material due its extremely low viscosity. Here, promising electro‐assisted bioprinting method developed, which can print low‐concentration pure GelMA microdroplets with cost, cell damage, and high efficiency. With the help of electrostatic attraction, uniform measuring about 100 μm are...
Here, a novel direct ink printing method was developed to print high-strength chitosan hydrogel scaffolds without any organic solvents.
Abstract Hydrogel microspheroids are widely used in tissue engineering, such as injection therapy and 3D cell culture, among which, heterogeneous drawing much attention a promising tool to carry multiple types separated phases. However, it is still big challenge fabricate that can reconstruct built‐up tissues' microarchitecture with excellent resolution spatial organization limited sizes. Here, novel airflow‐assisted bioprinting method reported, which print versatile spiral...
The mechanical cues of the external microenvironment have been recognized as essential clues driving cell behavior. Although intracellular signals modulating fate during sensory epithelium development is well understood, force formation remains elusive. Here, we manufactured a hybrid hydrogel with tunable properties for cochlear organoids culture and revealed that extracellular matrix (ECM) drives through shifting stiffness in stage-dependent pattern. As force, moderate ECM activated...
Although it has been well established that G protein plays pivotal roles in physiologic or pathologic conditions, including cancer formation, its role breast cancer, especially specific subunits, remains largely unknown. Our work aimed to evaluate the correlation of alpha subunit (GNAS) with and investigate underlying molecular mechanism.The expression GNAS was determined by tumor tissue microarray 150 patients complete follow-up information. The between clinical features assessed. CCK8, EdU...
Cancer immunotherapy has revolutionized the field of cancer treatment in recent years. However, not all patients receiving exhibit durable responses, and reliable, high-throughput testing platforms are urgently needed to guide personalized immunotherapy. The ability patient-derived tumor organoids recapitulate pivotal features original tissues makes them useful as a preclinical model for research precision medicine. Nevertheless, many challenges exist translation organoid clinical decision...
Liver cancer and other malignant tumor cells rely on the glycolytic pathway to obtain energy (i.e. Warburg effect); however, underlying mechanism is unclear. Mitochondria are sites of oxidative phosphorylation adenosine triphosphate (ATP) production. The 13 constituent respiratory chain proteins encoded by mitochondrial genome (namely, mtDNA) play essential roles. We found that in human hepatocellular carcinoma (HCC) tissues, 11 out mtDNA-encoded genes exhibited decreased mRNA levels 5...
Abstract Skin wound therapy aims not only to restore skin protection but also recover excitation functions through nerve regeneration. During the restoration of nerves, recruitment endogenous stem cells and promotion neuronal regeneration on site work stepwise are foundations in situ However, current therapeutic systems usually execute each process separately, leading limited recovery functions. Herein, a novel self‐adaptive all‐in‐one delivery chip (G:P:Al‐Chip) is constructed that combines...
Breast cancer (BC) is the most common invasive in women, and it imposes a heavy burden on patients. microRNAs (miRNAs/miRs) have been found to play an important role development of tumors, but their malignant progression BC unclear. In present study, expression level miR‑425‑5p was examined patients with BC, its association prognosis investigated. vitro experiments were performed examine cells. A downstream target gene predicted using miRNA prediction tool validated luciferase reporter...
Gelatin methacryloyl (GelMA) has become a popular biomaterial in the field of bioprinting. The derivation this material is gelatin, which hydrolyzed from mammal collagen. Thus, arginine-glycine-aspartic acid (RGD) sequences and target motifs matrix metalloproteinase (MMP) remain on molecular chains, help achieve cell attachment degradation. Furthermore, formation properties GelMA are versatile. methacrylamide groups allow to rapidly crosslinked under light irradiation presence...
Intrahepatic cholangiocarcinoma (iCCA) is closely correlated with hepatic progenitor cell (HPC) expansion and liver fibrosis. Brahma-related gene 1 (Brg1), an enzymatic subunit of the switch/sucrose nonfermentable complex that critical in stem maintenance tumor promotion, prominently up-regulated both HPCs iCCA; however, its role this correlation remains undefined.A retrospective cohort study indicated high Brg1 expression suggests poor prognosis patients iCCA. In chronically injured livers...
Daucosterol is a phytosterol glycoside with hepatoprotective properties. The objective of the present study was to confirm role daucosterol in liver failure. Exosomes were isolated from primary mouse umbilical cord mesenchymal stem cells (UCMSCs). A failure model generated by injecting lipopolysaccharide/D-galactosamine. Mice treated exosomes alone or combination (5, 10, 20 mg/kg). Liver tissue damage examined hematoxylin-eosin, Masson's trichrome, and TUNEL staining. levels genes, proteins,...
This review introduces different methods used to generate microgels and the microgel-based bioink for bioprinting. Besides, further tendency of microgel development in future is predicted.
Phyllanthus emblica L. fruits (PEFs) were processed by ultra-pressure (UHP) treatment and then extracted the ultrasonic-assisted extraction method. The influence of UHP on phenolic composition, enzyme inhibitory activity antioxidant free, esterified, bound fractions from PEFs compared. pretreatment significantly increased total flavonoid contents (p < 0.05). A 24 chemical compositions characterized in normal UHP-treated UHPLC-ESI-HRMS/MS. Compared with PEFs, these three different had...
Accurately reconstructing the intricate structure of natural organisms is long-standing goal 3-dimensional (3D) bioprinting. Projection-based 3D printing boasts highest resolution-to-manufacturing time ratio among all 3D-printing technologies, rendering it a highly promising technique in this field. However, achieving standardized, high-fidelity, and high-resolution composite structures using bioinks with diverse mechanical properties remains marked challenge. The root challenge lies neglect...