Abstract As the biocompatible materials, hydrogels have been widely used in three- dimensional (3D) bioprinting/organ printing to load cell for tissue engineering. It is important precisely control deposition during mimic organ structures. However, printability of about parameters seldom addressed. In this paper, we systemically investigated from lines (one dimensional, 1D structures) lattices/films (two 2D and 3D structures with a special attention accurate printing. After series...

In this study, 3D hydrogel-based vascular structures with multilevel fluidic channels (macro-channel for mechanical stimulation and microchannel nutrient delivery chemical stimulation) were fabricated by extrusion-based three-dimensional (3D) bioprinting, which could be integrated into organ-on-chip devices that would better simulate the microenvironment of blood vessels. approach, partially cross-linked hollow alginate filaments loading fibroblasts smooth muscle cells extruded through a...

Photo-crosslinkable gelatin methacrylate (GelMA) has become an attractive ink in 3D printing due to its excellent biological performance. However, limited by low viscosity and long cross-linking time, it is still a challenge directly print GelMA extrusion-based printing. Here, balance the printability biocompatibility, biomaterial composed of nanoclay was specially designed. Using this ink, complex scaffolds with high shape fidelity can be easily printed based on thixotropic property...

3D printing of silicone elastomers with the direct ink writing (DIW) process has demonstrated great potential in areas as diverse flexible electronics, medical devices, and soft robotics. However, most current silicones are not printable because their low viscosity long curing time. The lack systematic research on materials, processes during makes it a huge challenge to apply DIW more deeply widely. In this report, aiming at dilemmas processes, we proposed comprehensive guide for highly...

In this work, we have developed a mild route to fabricate typically mesoporous Mo2C–C hybrid nanospheres based on solvothermal synthesis and reduction–carbonization process. This work opens low-temperature synthesize valuable carbides. The resultant hybrid, for the first time, is used as an anode material in lithium ion batteries (LIBs). Compared with bulk Mo2C, exhibits much better electrochemical performance. Remarkably, electrode can deliver specific capacity of over 670 mA h g−1 after 50...

Abstract Liquid‐metal (LM)‐based flexible and stretchable electronics have attracted widespread interest in wearable computing, human–machine interaction, soft robotics. However, many current examples are one‐off prototypes, whereas future implementation requires mass production. To address this critical challenge, an integrated multimaterial 3D printing process composed of direct ink writing (DIW) sealing silicone elastomer special LM‐silicone (LMS) inks for manufacturing high‐performance...

Methods in nerve tissue engineering for peripheral regeneration have recently focused on the application of rapid three-dimensional (3D) printing with resorbable and degradable biomaterial ink. The technique aims to design fabricate guidance conduits (NGCs) repair large-gap injuries. In this study, Digital light processing (DLP)-based multichannel NGCs by using gelatin methacrylate (GelMA) hydrogels was presented. Multichannel different inner diameters were successfully fabricated desired...

Three-dimensional (3D) bioprinting of soft large-scale tissues in vitro is still a big challenge due to two limitations, (i) the lack an effective way print fine nutrient delivery channels (NDCs) inside cell-laden structures above millimetre level; (ii) need for feasible strategy vascularize NDCs. Here, novel 3D method reported directly with effectively vascularized Bioinks desired tissue cells and endothelial (ECs) are separately simultaneously printed from outside (mixed GelMA) gelatin)...

Gelatin methacryloyl (GelMA), a photocurable hydrogel, is widely used in 3D culture, particularly bioprinting, due to its high biocompatibility, tunable physicochemical properties, and excellent formability. However, as the properties performances of GelMA vary under different synthetic conditions, there lack standardization, leading conflicting results. In this study, uniform standard established understand enhance applications. First, basic concept density molecular network (DMN) are...

Tough hydrogel has attracted considerable interest in various fields, however, due to poor biocompatibility, nondegradation, and pronounced compositional differences from natural tissues, it is difficult be used for tissue regeneration. Here, a gelatin-based tough (GBTH) proposed fill this gap. Inspired by human exercise improve muscle strength, the synergistic effect utilized generate highly functional crystalline domains resisting crack propagation. The GBTH exhibits excellent tensile...

The use of microscale fibers could facilitate nutrient diffusion in fiber-based tissue engineering and improve cell survival. However, order to build a functional mini such as muscle fibers, nerve conduits, blood vessels, hydrogel microfibers should not only mimic the structural features native tissues but also offer cell-favorable environment sufficient strength for functionalization. Therefore, an important goal is fabricate morphology-controllable with appropriate materials complexity...

Abstract Hydrogel structures equipped with internal microchannels offer more in vivo‐relevant models for construction of tissues and organs vitro. However, currently used microfabrication methods constructing microfluidic devices are not suitable the handling hydrogel. This study presents a novel method fabricating hydrogel‐based chips by combining casting bonding processes. A twice cross‐linking strategy is designed to obtain interface that has same strength hydrogel bulk, which can be...

Liquid metal (LM)-based flexible sensors, which utilize advanced liquid conductive materials to serve as sensitive elements, are emerging a promising solution measure large deformations. Nowadays, one of the biggest challenges for precise control soft robots is detection their real-time positions. Existing fabrication methods unable fabricate sensors that match shape robots. In this report, we first described novel 3D printed multifunction inductance and stretchable sensor with LMs, capable...

Abstract Desktop three-dimensional (3D) printers (D3DPs) have become a popular tool for fabricating personalized consumer products, favored low cost, easy operation and other advantageous qualities. This study focused on the potential using D3DPs to successfully, rapidly economically print customized implants at medical clinics. An experiment was conducted D3DP-printed anterior cruciate ligament surgical implant rabbit model. A well-defined, orthogonal, porous PLA screw-like scaffold...

Cell-scaffold interactions have drawn significant attention in tissue engineering. In this study, we define a heterogeneous scaffold, which can be customized with respect to gradient strength, variable fiber diameter, and pore sizes. Heterogeneous scaffolds ultrafine fibers (3–22 μm diameter) printed via high-resolution melt electrowriting (MEW) using only one nozzle by adjusting the printing parameters. With precise control of diameter deposited fibers, complex patterns, such as star, Tai...

Abstract Low‐concentration gelatin methacryloyl (GelMA) has excellent biocompatibility to cell‐laden structures. However, it is still a big challenge stably fabricate organoids (even microdroplets) using this material due its extremely low viscosity. Here, promising electro‐assisted bioprinting method developed, which can print low‐concentration pure GelMA microdroplets with cost, cell damage, and high efficiency. With the help of electrostatic attraction, uniform measuring about 100 μm are...

Melt electro writing (MEW) provides three-dimensional (3D) printing porous scaffolds with well-defined geometrical features of ultrafine fibers in the tissue engineering. Scaffolds adjustable Poisson's ratio are more suitable certain biological applications for mimicking behavior native mechanics. However, it is still a challenging issue to tune ratio. In order resolve this problem, new method proposed present study design and fabricate tunable auxetic through altering configurations MEW....

Since projection-based 3D bioprinting (PBP) could provide high resolution, it is well suited for printing delicate structures tissue regeneration. However, the low crosslinking density and photo-crosslinking rate of photocurable bioink make difficult to print fine structures. Currently, an in-depth understanding lacking. Here, a research framework established analysis printability during PBP. The gelatin methacryloyl (GelMA)-based used as example, systematically investigated. We analyze...

Gelatin hydrogel based flexible electronics with liquid metal are proposed, which could be used as body surface sensor to monitor human heart rate during exercise and implantable heartbeat.

Implantable hydrogel-based bioelectronics (IHB) can precisely monitor human health and diagnose diseases. However, achieving biodegradability, biocompatibility, high conformality with soft tissues poses significant challenges for IHB. Gelatin is the most suitable candidate IHB since it a collagen hydrolysate substantial part of extracellular matrix found naturally in tissues. This study used 3D printing ultrafine fiber networks metamaterial design to embed into ultra-low elastic modulus...

The mechanical cues of the external microenvironment have been recognized as essential clues driving cell behavior. Although intracellular signals modulating fate during sensory epithelium development is well understood, force formation remains elusive. Here, we manufactured a hybrid hydrogel with tunable properties for cochlear organoids culture and revealed that extracellular matrix (ECM) drives through shifting stiffness in stage-dependent pattern. As force, moderate ECM activated...