A5029916604- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Ferroptosis and cancer prognosis
- Acute Ischemic Stroke Management
- Barrier Structure and Function Studies
- Cholesterol and Lipid Metabolism
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Microfluidic and Bio-sensing Technologies
- Renal cell carcinoma treatment
- Retinoids in leukemia and cellular processes
- Alcohol Consumption and Health Effects
- Mitochondrial Function and Pathology
- Health, Environment, Cognitive Aging
- Seaweed-derived Bioactive Compounds
- RNA Interference and Gene Delivery
- Traditional and Medicinal Uses of Annonaceae
- DNA Repair Mechanisms
- Tuberculosis Research and Epidemiology
- Extracellular vesicles in disease
- Histone Deacetylase Inhibitors Research
- Berberine and alkaloids research
- Microfluidic and Capillary Electrophoresis Applications
Sichuan University
2014-2025
West China Hospital of Sichuan University
2021-2024
State Key Laboratory of Biotherapy
2008-2023
National Center for Geriatrics and Gerontology
1999-2000
West China Medical Center of Sichuan University
1999
Nagoya University
1991-1994
Abstract Background Excessive circular fatty acid, particlarly saturated can result in insulin resistance skeletal muscle, but other adverse effects of acid accumulation myocytes remain unclear. Methods Differentiated C2C12 myotubes were used. The palmitate on cell viability, glucose uptake, gene expression and myotube loss evaluated by MTT assay, 2NBDG qRT-PCR, Western Blot crystal staining-based counting, respectively. In some expreiments, oleate was administrated, or the inhibitors...
Histone deacetylase inhibitor-induced expression of p21WAF1 is p53 independent. In the present study, we provide evidence that trichostatin A (TSA), a specific inhibitor histone deacetylase, can elevate H3 and H4 acetylation in NIH3T3 cells at first. To identify transcription factor which responsible for understand potential events occurred during this process, analyze response mouse promoter to TSA detail. The region responsive treatment p21 located -100 bp upstream from initiation site...
Apigenin, a naturally occurring, non-mutagenic flavonoid, has been shown to inhibit UV-induced skin tumorigenesis in mice when topically applied. In this report we have used the mouse keratinocyte 308 cell line, which contains wild-type p53 gene, study effect of apigenin treatment on protein levels and expression its downstream partner, p21/waf1. Cells were treated with 70 μM for various times p21/waf1 assessed by western blot analysis. The level was induced 27-fold after 4 h remained...
Abstract With the increase of aging population and prevalence obesity, incidence cardiovascular disease (CVD) cancer has also presented an increasing tendency. These two different diseases, which share some common risk factors. Relevant studies in field reversing Cardio-Oncology have shown that phenotype CVD a significant adverse effect on tumor prognosis, is mainly manifested by positive correlation between malignant progression concomitant tumors. This distal crosstalk link diseases makes...
Abstract The ambiguity of etiology makes temporomandibular joint osteoarthritis (TMJOA) “difficult-to-treat”. Emerging evidence underscores the therapeutic promise exosomes in management. Nonetheless, challenges such as low yields and insignificant efficacy current exosome therapies necessitate significant advances. Addressing lower strontium (Sr) levels arthritic synovial microenvironment, we studied effect Sr element on miRNA selectively loading mesenchymal stem cells (SMSCs). Here,...
We have used the yeast two-hybrid system to clone protein that interacts with BFCOL1 (binding factor of a type-I collagen promoter) zinc-finger transcription was cloned previously as binds two mouse proximal promoters genes. utilized bait N-terminal domain includes DNA-binding domain. One cDNA contained potential open reading frame for polypeptide 392 amino acids and identical PTRF (polymerase I transcript-release factor), which is involved in termination RNA polymerase reaction....
The use of vincristine, a known antitumor agent, in hepatoma therapy is limited particularly because its toxic effect. Meanwhile, berberine has drawn increasing attention to antineoplastic effect recent years. In view the advantages combinational drug treatment reported anti-cancer chemotherapy, we evaluated effects co-treatment vincristine and on hepatic carcinoma cell lines this study. We find that usage these two drugs can significantly induce growth inhibition apoptosis even under...
Phosphotyrosine‐containing proteins in various human cancer cell lines were studied by immunoblotting with anti‐phosphotyrosine antibody. Of 29 derived from oral epidermoid cancer, esophageal gastric colon pancreatic hepatocellular carcinoma and malignant melanoma, 3 of the 6 cells showed aberrant elevation tyrosine‐speciflc phosphorylation. On other hand, both cells, which reported to have amplified epidermal growth factor receptor activated p60 v‐src kinase, respectively, no apparent...
To explore the potential molecular mechanism underlying effect of green tea extract (TE), rich in polyphenols (TPs), on improving alcohol-induced liver injury.Mice were intragastrically treated with 50% (v/v) alcohol administration (15 ml/kg BW) or without three doses TE (50, 120 and 300 mg TPs/kg daily for 4 weeks, biological changes tested.The improved functional histological situations mice accepted administration, including enzymes metabolism, oxidative stress lipid accumulation....
The side population (SP) cells are identified through Hoechst 33342 staining and analyzed using flow cytometry (FCM). SP method is utilized for the isolation of stem based on dye efflux properties ATP-binding cassette (ABC) transporters. was initially employed identification hematopoietic (HSCs), but it has now evolved to primarily focus cancer (CSCs). traditional detection FCM uses a 355 nm laser excite detect cells. Through this study, we have successfully alternative approaches excitation...
Kidney renal clear cell carcinoma (KIRC) is one of the most hazardous tumors in urinary system. The regulation oxygen consumption a consequence adaptive reprogramming oxidative metabolism tumor cells. APPL1 signaling adaptor involved survival, stress, inflammation, and energy metabolism. However, correlation with regulatory T (Treg) infiltration prognostic value KIRC remain unclear. In this study, we comprehensively predicted potential function KIRC. For patients, relatively low expression...
The aim of this study was to explore the existence a relationship between rs189037 single nucleotide polymorphism (SNP) ataxia telangiectasia mutated (ATM) gene and cognitive impairment in elderly (aged 60 years above). In cohort, 505 residents Suinung City were consecutively recruited their function measured using 30-point Mini-Mental State Examination (MMSE). subjects divided into group control on basis MMSE scores. Presence SNP variant examined polymerase chain reaction-restriction...