A5026430459- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Immune Cell Function and Interaction
- Angiogenesis and VEGF in Cancer
- Zebrafish Biomedical Research Applications
- Cell Adhesion Molecules Research
- Developmental Biology and Gene Regulation
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Chemokine receptors and signaling
- Congenital heart defects research
- Mesenchymal stem cell research
- Immune Response and Inflammation
- Bone and Dental Protein Studies
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Invertebrate Immune Response Mechanisms
- Cancer-related gene regulation
- Retinoids in leukemia and cellular processes
- Lymphatic System and Diseases
- Proteoglycans and glycosaminoglycans research
- dental development and anomalies
Oslo University Hospital
2008-2017
University of Oslo
2008-2017
Medical University of Vienna
2012
Norwegian Institute of Public Health
2012
Bærum Sykehus
2012
Royal Netherlands Academy of Arts and Sciences
2011
Exelixis (United States)
2006-2010
Max Planck Institute for Developmental Biology
2002-2007
A number of studies have suggested that retinoic acid (RA) is an important signal for patterning the hindbrain, branchial arches and limb bud. Retinoic thought to act on posterior hindbrain buds at somitogenesis stages in chick mouse embryos. Here we report a much earlier requirement RA signalling during pre-segmentation proper development these structures zebrafish. We present evidence necessary expression spinal cord markers hoxb5a hoxb6b, suggesting influence anteroposterior neural plate...
The subdivision of the lateral mesoderm into a visceral (splanchnic) and somatic layer is crucial event during early development in both arthropod vertebrate embryos. In Drosophila, this leads to differential gut musculature versus body wall musculature. Here we report that biniou, sole Drosophila representative FoxF subfamily forkhead domain genes, has key role derived biniou expression activated trunk primordia downstream dpp, tinman, bagpipe maintained all types developing muscles. We...
We have used a disc-shaped self-gelling alginate hydrogel as scaffold for in vitro chondrogenic differentiation of human bone marrow-derived mesenchymal stem cells. The comparison monolayer cells and embedded with or without medium allowed us to perform detailed kinetic study the expression range genes proteins known be involved chondrogenesis, using real-time polymerase chain reaction, fluorescence immunohistochemistry, glycosaminoglycan measurement supernatant. mRNA encoding type II...
Lesions of hyaline cartilage do not heal spontaneously, and represent a therapeutic challenge. In vitro engineering articular using cells biomaterials may prove to be the best solution. Patients with osteoarthritis (OA) require tissue engineered therapy. Chondrocytes obtained from OA joints are thought involved in disease process, thus insufficient quality used for repair strategies. Bone marrow (BM) derived mesenchymal stem (MSCs) healthy donors an alternative cell source. We have isolated...
Large-scale mutagenesis screens in the zebrafish employing mutagen ENU have isolated several hundred mutant loci that represent putative developmental control genes. In order to realize potential of such screens, systematic genetic mapping mutations is necessary. Here we report on a large-scale effort map generated screening at Max Planck Institute for Developmental Biology by genome scanning with microsatellite markers.We selected set markers and developed methods scoring criteria suitable...
SUMMARY Tie-2 is a member of the receptor tyrosine kinase family and required for vascular remodeling maintenance mammalian vessel integrity. A number mutations in human TIE2 gene have been identified patients suffering from cutaneomucosal venous malformations ventricular septal defects. How exactly signaling pathways play different roles both development stability unknown. We generated zebrafish line carrying stop mutation domain receptor. Mutant embryos lack protein, but do not display any...
Interleukin (IL)-33 is a nuclear protein that released from stressed or damaged cells to act as an alarmin. We investigated the effects of IL-33 on endothelial cells, using prototype IL-1 family member, IL-1β, reference.Human umbilical vein were stimulated with showing highly similar phosphorylation signaling molecules, induction adhesion and transcription profiles. However, intradermally injected elicited significantly less proinflammatory activation when compared IL-1β led us observe...
Vascular endothelial cells present luminal chemokines that arrest rolling leukocytes by activating integrins. It appears several must form higher-order oligomers to elicit proper in vivo effects, as mutants restricted forming dimers have lost the ability recruit sites of inflammation. Here, we show for first time chemokine RANTES/CCL5 binds surface human a regular filamentous pattern. Furthermore, filaments bound heparan sulfate-dependent manner. By electron microscopy observed labeling...
Sorting of proteins to Weibel-Palade bodies (WPB) endothelial cells allows rapid regulated secretion leukocyte-recruiting P-selectin and chemokines as well procoagulant von Willebrand factor (VWF). Here we show by domain swap studies that the exposed aspartic acid in loop 2 (Ser44-Asp45-Gly46) CXC chemokine interleukin (IL)-8 is crucial for targeting WPB. Loop also governs sorting α-granules platelets, but fingerprint these differs from IL-8. On other hand, IL-8 closely resembles a...
The future ability to manipulate the growth of new blood vessels (angiogenesis) holds great promise for treating ischemic disease and cancer. Several models human in vivo angiogenesis have been described, but they seem depend on transgenic support not validated a therapeutic context. Here, we describe an assay that mimics which native umbilical vein-derived endothelial cells are suspended liquid laminin/collagen gel (Matrigel), injected into immunodeficient mice, develop mature, functional...
IL-33, required for viral clearance by cytotoxic T cells, is generally expressed in vascular endothelial cells healthy human tissues. We discovered that IL-33 expression was stimulated as a response to adenoviral transduction. This dependent on MRE11, sensor of DNA damage can also be activated DNA, and IRF1, transcriptional regulator cellular responses invasion damage. Accordingly, we observed responded phosphorylation ATM CHK2 depletion or inhibition but not ATM, abrogated stimulation. In...
Tie-2 is a member of the receptor tyrosine kinase family and required for vascular remodeling maintenance mammalian vessel integrity.A number mutations in human TIE2 gene have been identified patients suffering from cutaneomucosal venous malformations ventricular septal defects.How exactly signaling pathways play different roles both development stability unknown.We generated zebrafish line carrying stop mutation domain receptor.Mutant embryos lack protein, but do not display any defect...
Abstract The molecular mechanisms that induce and maintain expression of the alarming interleukin-33 (IL-33) in endothelial cells are unknown. Based on observation IL-33 is also a marker cell quiescence, we hypothesized Notch signaling pathway might be involved regulation expression. Here show activation Notch1 by immobilized ligands was sufficient to nuclear human umbilical vein (HUVECs). Furthermore, confluent HUVECs inhibited γ-secretase inhibitor DAPT or blocking antibodies Dll4, Jagged1...