Datasets containing recorded landslide and non-landslide samples can greatly influence the performance of machine learning (ML) models, which are commonly used in susceptibility mapping (LSM). However, cannot be directly obtained. In this study, a pattern-based approach is proposed to improve LSM process, constructing unsupervised (UML) – supervised (SML) collaborative models reasonably selected. Two UML Gaussian mixture model (GMM) K-means, introduced sample datasets with four sampling...

N7-methylguanosine (m7G) modification is, a more common epigenetic in addition to m6A modification, mainly found mRNA capsids, interiors, transfer RNA (tRNA), pri-miRNA, and ribosomal (rRNA). It has been that m7G modifications play an important role transcription, tRNA stability, rRNA processing maturation, miRNA biosynthesis. However, the of within its "writer" methyltransferase 1(METTL1) tumors, particularly prostate cancer (PCa), not revealed.The differential expression level METTL1...

Abstract As the most common modification of RNA, N 6 -methyladenosin (m A) has been confirmed to be involved in occurrence and development various cancers. However, relationship between m A castration resistance prostate cancer (CRPC), not fully studied. By A-sequencing patient tissues, we identified that overall level CRPC was up-regulated than sensitive (CSPC). Based on analysis data, found HRas proto-oncogene, GTPase (HRAS) mitogen-activated protein kinase 2 (MEK2 or MAP2K2) were enhanced...

Abstract The refractory of castration-resistant prostate cancer (CRPC) is mainly reflected in drug resistance. current research on the resistance mechanism CRPC still its infancy. In this study, we revealed for first time key role LncRNA PCBP1-AS1 Through detailed vivo and vitro studies, found that may enhance deubiquitination AR/AR-V7 by stabilizing USP22-AR/AR-V7 complex, thereby preventing from being degraded through ubiquitin–proteasome pathway. Targeting can significantly restore...

The mutation rate used in the previous analyses of pig evolution and demographics was cursory hence invited potential bias inferring evolutionary history. Herein, we estimated de novo pigs as 3.6 × 10-9 per base generation using high-quality whole-genome sequencing data from nine individuals a three-generation pedigree through stringent filtering validation. Using this rate, re-investigated history pigs. divergence time ∼ 10 kiloyears ago (KYA) between European wild domesticated consistent...

Summary Under natural farming, environmental pathogenic microorganisms may invade and affect swine lungs, further resulting in lung lesions. However, few studies on microbiota their potential relationship with lesions were reported. Here, we sampled 20 pigs from a hybrid herd raised under conditions; recorded lung‐lesion phenotype investigated microbial communities by sequencing the V3‐V4 region of 16S rRNA gene for each individual. We found reduced diversity but more biomass severe‐lesion...

Abstract Castration-resistant prostate cancer (CRPC) is a highly malignant type of advanced resistant to androgen deprivation therapy. One the important mechanisms for development CRPC persistent imbalanced regulation AR and splice variants (AR/AR-Vs). In this study, we reported KDM4A-AS1, recently discovered lncRNA, as tumor promoter that was significantly increased in cell lines tissues. Depletion KDM4A-AS1 reduced viability, proliferation, migration vitro, growth vivo. We found by binding...

Wild boars inhabit diverse climates, including frigid regions like Siberia, but their migration history and cold adaptation mechanisms into high latitudes remain poorly understood. We constructed the most comprehensive wild boar whole-genome variant dataset to date, comprising 124 samples from tropical zones, among which 47 Russian, 8 South Chinese 3 Vietnamese were newly supplemented. also gathered 75 high-quality RNA-seq datasets 10 tissues of 6 Russia southern China. Demographic analysis...

Enzalutamide, a second-generation androgen receptor (AR) antagonist, has represented the association with improved overall survival in men prostate cancer (PCa). However, PCa patients receiving enzalutamide will eventually develop resistance through various mechanisms without effective regimens. Here, we observed higher level of formin-like 2 (FMNL2) enzalutamide-resistant cells. Functionally, FMNL2 knockdown partially re-sensitized Mechanistically, directly interacted SRC kinase FMNL2-FH1...

This study focused on large‐scale roof‐fall accidents occurred in large‐section coal seam roadways of Bayangaole Coal Mine, Inner Mongolia, China, and investigated the occurrence mechanism related supporting control method detail. Firstly, fracture characteristics surrounding rocks roadway roof were measured using a stratum detector. The results showed that underwent most severe failure with maximum deformation 3.53 m; bedding separation zones distributed at irregular intervals. Accordingly,...

Abstract Throughout its distribution across Eurasia, domestic pig (Sus scrofa) populations have acquired differences through natural and artificial selection, often interbred. We resequenced 80 Eurasian pigs from nine different Asian European breeds; we identify 42,288 reliable SNPs on the Y chromosome in a panel of 103 males, among which 96.1% are newly detected. Based these new data, elucidate evolutionary history lens chromosome. two highly divergent haplogroups: one present only Asia...

Abstract Background Short tandem repeats (STRs) are genetic markers with a greater mutation rate than single nucleotide polymorphisms (SNPs) and widely used in studies forensics. However, most pigs have focused only on SNPs or limited number of STRs. Results This study screened 394 deep-sequenced genomes from 22 domesticated pig breeds/populations worldwide, wild boars both Europe Asia, numerous outgroup Suidaes, identified set 878,967 polymorphic STRs (pSTRs), which represents the largest...

To explore the similar and different pathogenesis between non-small cell lung cancer (NSCLC) small (SCLC).This study used bioinformatics methods, including functional enrichment analysis, compared topological features of SCLC NSCLC in human protein interaction network a system aspect, analyzed highly intense modules from an integrated network.This included 5082 2781 significantly expression genes for SCLC, respectively. The differently expressed are mainly distributed extracellular region...

Wrinkles are uneven concave-convex folds, ridges or creases in skin. Facial wrinkles appear head, typically increasing along with aging. However several Chinese indigenous pigs, such as Erhualian rich facial have been generated during the growth stages one of their breed characteristics. To investigate genetic basis underlying development swine wrinkles, we estimated folding extent a herd pigs (n=332), and then conducted genome-wide association studies multi-trait meta-analysis for using 60K...

We investigated the relationship between small cell lung cancer (SCLC) and non-small (NSCLC) based on micro ribonucleic acid (miRNA) messenger (m)RNA expression profiles.Utilizing differentially expressed mRNAs targeting miRNAs, mRNA-miRNA network for two cancers was constructed. By integrating miRNA profile, drug, drug targets, miRNA-drug target-drug networks were established mechanisms in therapy efficacy compared SCLC NSCLC.Drug targets of different miRNAs are mainly located organelle,...

Abstract Structural variations (SVs) are important DNA polymorphisms that contribute to genetic diversity and evolution in humans, animals, plants. In this study, we present a novel swine SV dataset of 79,919 deletions, 23,638 duplications, 9333 inversions with average sequence depths 24.1× from 24 varieties worldwide pig populations, encompassing 305 individuals. Genotypes SVs, particularly can accurately group individuals based on their population identity. We showed exon‐covering...

Swine respiratory disease (SRD) causes massive economic losses in the swine industry and is difficult to control eradicate on pig farms. Here, we employed population genetics transcriptomics approaches decipher molecular mechanism of host adaptation disease. We recorded two SRD-related traits, enzootic pneumonia-like (EPL) score lung lesion (LL) levels, performed four body weight measurements, at ages 150, 180, 240, 300 days, a Chinese Bamaxiang herd (n = 314) raised under consistent indoor...

Abstract Understanding the community structure and functional capacity of lower respiratory tract microbiome is crucial for elucidating its roles in diseases. However, there are few studies about it owing to difficulty obtaining microbial samples from tract. Here we collected 745 porcine by harvesting 675 pigs, constructed a gene catalog containing 10,337,194 nonredundant genes, which only 30% could be annotated taxonomically. We obtained 397 metagenome-assembled genomes (MAGs) including 111...

Abstract The mutation rate used in the previous analyses of pig evolution and demographics was cursory brought potential bias inferring its history. Herein, we estimated de novo pigs using high-quality whole-genome sequencing data from nine individuals a three-generation pedigree through stringent filtering validation. 3.6 × 10 −9 per generation, corresponding to 1.2 site year. Using this rate, re-investigated evolutionary history pigs. Our estimates agreed divergence time ~10 kiloyears ago...