A5108062588- Immune Cell Function and Interaction
- Lipoproteins and Cardiovascular Health
- Phagocytosis and Immune Regulation
- Gut microbiota and health
- T-cell and B-cell Immunology
- Acute Ischemic Stroke Management
- Helicobacter pylori-related gastroenterology studies
- Cancer Research and Treatments
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Clostridium difficile and Clostridium perfringens research
- Single-cell and spatial transcriptomics
- Immune Response and Inflammation
- Antibiotic Use and Resistance
- Cancer Cells and Metastasis
- Intracerebral and Subarachnoid Hemorrhage Research
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Hepatitis B Virus Studies
- Graphene and Nanomaterials Applications
- Melanoma and MAPK Pathways
- Sulfur Compounds in Biology
- HIV, Drug Use, Sexual Risk
- Galectins and Cancer Biology
Shandong First Medical University
2020-2022
Shandong Provincial Hospital
2020-2022
Shandong University
2022
The First People's Hospital of Wenling
2020
Wenzhou Medical University
2020
The University of Texas Medical Branch at Galveston
2018-2020
Ministry of Agriculture and Rural Affairs
2018
Kaifeng University
2014
Beth Israel Deaconess Hospital
2012
Harvard University
2010
T-cells are crucial in maintanence of intestinal homeostasis, however, it is still unclear how microbiota metabolites regulate T-effector cells. Here we show gut microbiota-derived short-chain fatty acids (SCFAs) promote antigen-specific Th1 cell IL-10 production, mediated by G-protein coupled receptors 43 (GPR43). Microbiota Gpr43-/- CBir1 transgenic (Tg) cells, specific for antigen flagellin, induce more severe colitis compared with wide type (WT) Tg cells Rag-/- recipient mice. Treatment...
How the gut microbiota regulates intestinal homeostasis is not completely clear. Gut metabolite short-chain fatty acids (SCFAs) have been reported to regulate T-cell differentiation. However, mechanisms underlying SCFA regulation of differentiation and function remain be investigated.CBir1, an immunodominant antigen, transgenic T cells were treated with butyrate under various polarization conditions investigate mechanism involved. Transfer butyrate-treated CBir into Rag1-/- mice was...
Background and AimsGut bacteria-derived short-chain fatty acids (SCFAs) play crucial roles in the maintenance of intestinal homeostasis. However, how SCFAs regulate epithelial turnover tissue repair remain incompletely understood. In this study, we investigated SCFA propionate regulates cell migration to promote renewal repair.MethodsMouse small cells (MSIE) human Caco-2 were used determine effects on gene expression, proliferation, migration, spreading vitro. Video microscopy single...
Neutrophils are the first responders to sites of inflammation when intestinal epithelial barrier is breached and gut microbiota invade. Despite current efforts in understanding role neutrophils homeostasis, complex interactions between cells (IECs) still not well characterized. In this study, we demonstrated that enhanced production amphiregulin (AREG), a member EGFR ligand family, by IECs, which promoted IEC function tissue repair. Depletion resulted more severe colitis mice because...
Obesity is prevalent worldwide and a major risk factor for the development progression of kidney disease. Hydrogen sulfide (H 2 S) plays an important role in renal physiological pathophysiological processes. However, whether H S able to mitigate injury induced by obesity mice remains unclear. In this study, we demonstrated that significantly reduced accumulation lipids kidneys high fat diet‐ (HFD‐) obese mice. The results hematoxylin eosin, periodic acid‐Schiff, Masson’s trichrome staining...
Abstract EWS/FLI1-activated transcript 2 (EAT-2)A and EAT-2B are single SH2-domain proteins, which bind to phosphorylated tyrosines of signaling lymphocyte activation molecule family receptors in murine NK cells. While EAT-2 is a positive regulator human cells, negative regulatory role was attributed the adapter cells derived from EAT-2A–deficient 129Sv mice. To evaluate whether genetic background or presence selection marker mutant mice could influence mode these adapters, we generated...
Natural-killer group 2 (NKG2), a natural killer (NK) cell receptor, plays critical role in regulating NK cytotoxicity. In this study, we investigated the expression levels of member A (NKG2A) and D (NKG2D) cells as well regulatory function NKG2D patients with colorectal cancer (CRC). Sixty-two CRC 32 healthy controls were enrolled study. The NKG2A mRNA peripheral blood mononuclear (PBMCs) using real-time PCR. Flow cytometry was performed to assay proteins cells. PBMCs significantly lower...
Although the variation in chromatin architecture during adaptive immune responses has been thoroughly investigated, 3D landscape of innate immunity is still unknown. Herein, regulation and heterogeneity among human primary monocytes were investigated. Peripheral blood was collected from two healthy persons patients with systemic lupus erythematosus (SLE), CD14
Abstract Background: Studies have shown that CD4 + CD25 Foxp3 Treg cells suppress NKG2D expression on NK via a cell contact-dependent mechanism and increased TGF-β IL-10 production in some cancer models. We herein aimed to explore whether Tregs NKG2D-mediated cytotoxicity peripheral blood elucidate the exact underlying this phenomenon. Methods: To function of NKG2D, cultures were treated with an NKG2D-blocking antibody block these receptors. Additionally, TGF-β- IL-10-blocking antibodies...
Introduction: Antibiotic consumption is associated with the development of antibiotic resistance. Our aim was to study relationship between and carbapenem-resistant Klebsiella pneumoniae (CRKP) in three public general tertiary hospitals from 2011-2015 eastern, western central regions China.
 Methodology: Valid data were collected quarterly, expressed as defined daily dose (DDD) per 100 inpatient days (ID). Bacterial resistance reported percentage resistant isolates among all tested...
Melanoma is the deadliest skin tumor and prone to distant metastases. The incidence of melanoma has increased rapidly in past few decades, current trends indicate that this growth continuing. This study was aimed explore molecular mechanisms pathogenesis discover underlying pathways genes associated with melanoma. We used high-throughput expression data differential profiles related differentially expressed (DEGs) GSE15605, GSE46517, GSE7553, Cancer Genome Atlas (TCGA) datasets were...
The disruption of blood–brain barrier (BBB) is a critical event in the formation brain edema during early phases ischemic injury. Poly(ADP-ribose) polymerase (PARP) activation, which contributes to BBB damage, has been reported ischemia–reperfusion and traumatic Here, we investigated effect 3-aminobenzamide (3-AB), PARP-1 inhibitor, on ultrastructure BBB. Male Sprague Dawley rats were suffered from 90 minutes middle cerebral artery occlusion, followed by 4.5 hours or 22.5 reperfusion (R)....
Abstract Gut microbiota greatly impacts host immune response and intestinal homeostasis. However, the mechanisms are still largely unclear. Short chain fatty acids (SCFAs), most abundant metabolites in intestines derived from gut bacterial fermentation of dietary fibers, either existing free lumen or being absorbed into circulation, perform multiple functions maintenance epithelial integrity homeostasis, have been shown to directly induce Treg cells ameliorate colitis. low concentration...
Abstract The mechanisms by which gut microbiota regulate intestinal homeostasis are still largely unknown. short chain fatty acids (SCFA), mainly acetate, propionate, and butyrate, bacterial metabolites produced from the fermentation of dietary fibers, have been shown to homeostasis. In this study, we investigated whether SCFAs promote epithelial repair wound healing through promoting cell production milk fat globulin E8 (MFG-E8), a known molecule migration via actin reorganization....
Abstract How microbiota regulate intestinal homeostasis is still not completely understood. Emerging evidence indicates that gut metabolites play crucial roles in maintenance of and regulation Inflammatory Bowel Disease (IBD). Many patients with IBD are deficient the microbial dietary fibers, short chain fatty acids (SCFAs). To date, for use SCFAs as a treatment has been inconsistent, highlighting need further investigation. In this study, we demonstrate promote epithelial cell (IEC) wound...
Objective To study the clinical value of combined detection tissue polypeptide antigen(TPA) and human epididymal secretory protein 4 (HE4) in diagnosis disease monitoring for ovarian epithelial cancer.Methods 82 cases with cancer were enrolled group,93 benign lesions selected as group,100 healthy people control group.Serum TPA HE4 detected by ELISA.Results TPA-positive rates group,benign group 70.7％,8.6％ 2.0％,respectively.The rate was higher than (x2 =33.69,82.95,all P ＜ 0.01).But difference...
Abstract Phagocytosis is a pivotal process by which macrophages eliminate microorganisms upon recognition pathogen sensors. Surprisingly, the self-ligand cell surface receptor Slamf1 functions not only as co-stimulatory molecule but also microbial sensor controlling killing of Gram-negative bacteria macrophages. regulates activity Nox2 enzyme and phagolysosomal maturation entering phagosome, recruiting Vps34, produces PI3P. Here we report that in mouse macrophages, interacted with Beclin 1...
Objective To investigate the effects of nimodipine combined with mannitol on serum interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and renal function in elderly patients hypertensive intracerebral hemorrhage (HICH). Methods Ninety-four HICH were divided into single group according to treatment regimen 47 each group. The was treated + nimodipine. two groups observed, recovery neurological (NIHSS), changes IL-1β, TNF-α levels [blood creatinine (Cr), urea nitrogen (BUN)]before...