A5100443159- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Receptor Mechanisms and Signaling
- Genomics and Chromatin Dynamics
- Neuropeptides and Animal Physiology
- Retinoids in leukemia and cellular processes
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Advancements in Battery Materials
- Neuroscience and Neuropharmacology Research
- Renal and related cancers
- Cell death mechanisms and regulation
- Neurotransmitter Receptor Influence on Behavior
- Fibroblast Growth Factor Research
- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Tea Polyphenols and Effects
- Protein Kinase Regulation and GTPase Signaling
- Gene Regulatory Network Analysis
- Bacteriophages and microbial interactions
- Genomic variations and chromosomal abnormalities
Shanghai First People's Hospital
2021-2025
Shanghai Jiao Tong University
2018-2025
Tianjin Medical University General Hospital
2025
Northwest University
2022-2025
Xi'an Jiaotong University
2015-2023
Chongqing Jiaotong University
2023
Uplift Education
2022
Harvard University
2018-2021
Kunming Medical University
2021
Xiamen University
2021
Canine parvovirus (CPV) type 2 emerged in 1978 the USA and quickly spread among dog populations all over world with high morbidity. Although CPV is a DNA virus, its genomic substitution rate similar to some RNA viruses. Therefore, it important trace evolution of monitor appearance mutations that might affect vaccine effectiveness. Our analysis shows VP2 genes isolated from 1979 2016 are divided into six groups: GI, GII, GIII, GIV, GV, GVI. Amino acid mutation revealed several undiscovered...
We recently discovered 27 recurrent DNA double-strand break (DSB) clusters (RDCs) in mouse neural stem/progenitor cells (NSPCs). Most RDCs occurred across long, late-replicating RDC genes and were found only after mild inhibition of replication. share intriguing characteristics, including encoding surface proteins that organize brain architecture neuronal junctions, are genetically implicated neuropsychiatric disorders and/or cancers. identification relies on high-throughput genome-wide...
Abstract Syndecan‐1, ‐2, ‐3, and ‐4 are heparan sulfate proteoglycans that differentially expressed during development wound repair. To determine whether syndecans also involved in brain injury, we examined the expression of syndecan core proteins genes cryo‐injured mouse brain, using situ hybridization. All mRNA transcripts were similarly region surrounding necrotic tissue, exhibiting peak levels at day 7 after injury. Comparison with cellular markers showed reactive astrocytes primary...
Non-obstructive azoospermia (NOA) is one of the most severe type in male infertility, and genetic causes NOA with meiotic arrest remain elusive.Four Chinese families participated study. We performed whole-exome sequencing (WES) for four NOA-affected patients pedigrees. The candidate causative gene was further verified by Sanger sequencing. Hematoxylin eosin staining (H&E) immunohistochemistry (IHC) were carried out to evaluate stage spermatogenesis arrested NOA.We identified two novel...
<title>Abstract</title> <bold>Backgroud</bold>: A frequently encountered breathing condition, obstructive sleep apnea (OSA) primarily manifests while sleeping and is characterized by total or incomplete blockage of the upper respiratory tract. This disorder disrupts normal airflow, often leading to repeated pauses in throughout night. Aging significantly increases risk OSA, yet underlying biomolecular connections between aging OSA remain incompletely understood. <bold>Methods</bold>:...
Uniform testicular maturation arrest is a severe form of male infertility characterized by the presence germ cells that do not complete spermatogenic development. It usually caused meiotic with genetic variants and difficult to treat via drugs or surgery. mRNA-lipid nanoparticle (LNP) delivery promising therapeutic option for monogenic protein replacement therapy. Herein, spermatocytes-tropic LNP (Pool1-LNP3) was identified library 30 ionizable lipids screening. And in vivo this novel...
Abstract Nonobstructive azoospermia (NOA), one of the most severe types male infertility, etiology often remains unclear in cases. Therefore, this study aimed to detect four biallelic detrimental variants (0.5%) minichromosome maintenance domain containing 2 ( MCMDC2 ) genes 768 NOA patients by whole-exome sequencing (WES). Hematoxylin and eosin (H&E) demonstrated that deleterious caused meiotic arrest three (c.1360G>T, c.1956G>T, c.685C>T) hypospermatogenesis patient...
Introduction The current treaments for Obstructive Sleep Apnea Hypopnea (OSAHS) are Continuous Positive Airway Pressure (CPAP) and lifestyle modifications, which is not suitable all patients. Traditional Chinese medicine (TCM) has increasingly demonstrated its efficacy benefits in treating OSAHS. Zhihan Anshen Tang (ZHAST), been clinical metrics OSAHS However, key ingredients mechanisms of action still unknown. Methods Using network pharmacology, we investigated the potential ZHAST through...
ABSTRACT The whole genome sequences of A. dauci DSM 28700, fastidiosus KKP 3000 (guaiacol producers), and 17978 (non‐guaiacol producer) were firstly determined. Then, the presence guaiacol biosynthetic gene cluster in 7 guaiacol‐producing 16 non‐guaiacol producing Alicyclobacillus strains was explored. Of producers investigated, a complete found K3000, acidiphilus NBRC 100859, suci VF‐FSL‐W10‐0049 FSL‐W10‐0048; only guaB , guaC guaD guaE genes herbarius 13609, guaA hesperidum subsp. aegles...
Background Azoospermia, the most severe form of male infertility, is categorized into two types: non-obstructive azoospermia (NOA) and obstructive (OA), which exhibit significant genetic heterogeneity. Azoospermia factor (AZF) deletion a common cause NOA, whereas congenital bilateral absence vas deferens (CBAVD), subtype OA, frequently linked to cystic fibrosis transmembrane conductance regulator (CFTR) gene variants. This case report first document coexistence partial AZFa microdeletion...
<title>Abstract</title> Mitochondrial fatty acid β-oxidation (FAO) is critical for cellular energy homeostasis, yet its regulatory role in sperm remains elusive. Whole-exome sequencing of 800 asthenozoospermia patients revealed biallelic TEX44 mutations six individuals with defective mitochondrial sheath assembly and impaired motility. In Tex44 knockout mice, interacts CPT1B to form a "glue," securing adjacent mitochondria enabling formation. vitro, directly suppresses CPT1B-mediated...
Stepwise mini-incision microdissection testicular sperm extraction (mTESE) is a procedure that attempts to minimize damage. However, the approach may vary in patients with different etiologies. Here, we performed retrospective analysis of 665 men nonobstructive azoospermia (NOA) who underwent stepwise mTESE (Group 1) and 365 standard 2). The results showed operation time (mean ± deviation) for successful retrieval Group 1 (64.0 26.6 min) was significantly shorter than 2 (80.2 31.3 min), P...
Non-obstructive azoospermia (NOA) is the most severe disease in male infertility, but genetic causes for majority of NOA remain unknown. FANCM a member Fanconi Anemia (FA) core complex, whose defects are associated with cell hypersensitivity to DNA interstrand crosslink (ICL)-inducing agents. It was reported that variants (MIM: 609644) might cause or oligospermia. However, there still lack evidence explain association between different and infertility phenotypes. Herein, we identified...
Abstract Hypoxia inducible factor 1 alpha (HIF1A) is a transcription (TF) that forms highly structural and functional protein–protein interactions with other TFs to promote gene expression in hypoxic cancer cells. However, despite the importance of these TF-TF interactions, we still lack comprehensive view many TF cofactors involved how they cooperate. In this study, systematically studied HIF1A eight cell lines using computational motif mining tool, SIOMICS, discovered 201 potential...
Primate-specific DAZ (deleted in azoospermia) has evolved the azoospermia factor c (AZFc) locus on Y chromosome. Loss of is associated with patients deletion AZFc region (AZFc_del). However, molecular mechanisms spermatogenesis remain uncertain. In this study, mechanism identified, which unknown since it identified 40 years ago because lack a suitable model. Using clinical samples and cell models, shown that plays an important role loss defective proliferation c-KIT-positive spermatogonia...
Bilins are linear tetrapyrroles commonly used as chromophores of phycobiliproteins and phytochromes for light-harvesting or light-sensing in photosynthetic organisms. Many eukaryotic algae lack both phytochromes, but retain the bilin biosynthetic enzymes including heme oxygenase (HO/HMOX) ferredoxin-dependent biliverdin reductase (FDBR). Previous studies on Chlamydomonas reinhardtii mutant (hmox1) have shown that bilins not only essential retrograde signals to mitigate oxidative stress...
Mild replication stress enhances appearance of dozens robust recurrent genomic break clusters, termed RDCs, in cultured primary mouse neural stem and progenitor cells (NSPCs). Robust RDCs occur within genes (“RDC-genes”) that are long have roles cell communications and/or been implicated neuropsychiatric diseases or cancer. We sought to develop an vitro approach determine whether specific RDC formation is associated with development. For this purpose, we adapted a system induce (NPC)...
Abstract The genetic causes of idiopathic premature ovarian insufficiency (POI) and nonobstructive azoospermia (NOA) remain unclear. We performed whole‐exome sequencing (WES) in members a consanguineous family with two POI NOA patients to screen for potential pathogenic variants familial NOA. And homozygous variant SPATA22 (c.400C>T:p.R134X) was identified. Histological analysis spermatocyte spreading assay demonstrated that the spermatogenesis arrested at zygotene‐like stage proband...