A5101500789- Cardiac Imaging and Diagnostics
- Coronary Interventions and Diagnostics
- Hepatitis C virus research
- Hepatitis B Virus Studies
- SARS-CoV-2 and COVID-19 Research
- Cardiovascular Function and Risk Factors
- Cardiovascular Disease and Adiposity
- Liver Disease Diagnosis and Treatment
- Peripheral Artery Disease Management
- Cardiac Valve Diseases and Treatments
- Acute Myocardial Infarction Research
- Cerebrovascular and Carotid Artery Diseases
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Hormonal Regulation and Hypertension
- Hemodynamic Monitoring and Therapy
- Advanced X-ray and CT Imaging
- Diet and metabolism studies
- Cardiac, Anesthesia and Surgical Outcomes
- Congenital Anomalies and Fetal Surgery
- COVID-19 epidemiological studies
- Cardiac Structural Anomalies and Repair
- Reproductive tract infections research
- Gastrointestinal motility and disorders
- Uterine Myomas and Treatments
Onze Lieve Vrouwziekenhuis Hospital
2022-2025
Aichi Medical University
2019-2025
National Institute of Infectious Diseases
2013-2023
Memorial Hermann
2023
Tokyo University of Science
2015-2022
Cardiovascular Research Center
2022
Ministry of Health Labour and Welfare
2020
Jikei University School of Medicine
2018
Tosei General Hospital
2017
Mie Chuo Medical Center
1991-2009
Antiviral treatments targeting the coronavirus disease 2019 are urgently required. We screened a panel of already approved drugs in cell culture model severe acute respiratory syndrome 2 (SARS-CoV-2) and identified two new agents having higher antiviral potentials than drug candidates such as remdesivir chroloquine VeroE6/TMPRSS2 cells: anti-inflammatory cepharanthine human immunodeficiency virus protease inhibitor nelfinavir. Cepharanthine inhibited SARS-CoV-2 entry through blocking viral...
The scientific community is focused on developing antiviral therapies to mitigate the impacts of ongoing novel coronavirus disease 2019 (COVID-19) outbreak. This will be facilitated by improved understanding viral dynamics within infected hosts. Here, using a mathematical model in combination with published load data, we compare within-host SARS-CoV-2 analogous MERS-CoV and SARS-CoV. Our quantitative analyses revealed that reproduction number at symptom onset was statistically significantly...
Virus infection is restricted by intracellular immune responses in host cells, and this typically modulated stimulation of cytokines. The cytokines factors that determine the cell restriction against hepatitis B virus (HBV) are not well understood. We screened 36 chemokines to which were able reduce susceptibility HepaRG cells HBV infection. Here, we found pretreatment with IL-1β TNFα remarkably reduced This effect was mediated activation NF-κB signaling pathway. A cytidine deaminase,...
Summary Antiviral treatments targeting the emerging coronavirus disease 2019 (COVID-19) are urgently required. We screened a panel of already-approved drugs in cell culture model severe acute respiratory syndrome 2 (SARS-CoV-2) and identified two new antiviral agents: HIV protease inhibitor Nelfinavir anti-inflammatory drug Cepharanthine. In silico modeling shows binds SARS-CoV-2 main consistent with its inhibition viral replication, whilst Cepharanthine inhibits attachment entry into cells....
The incubation period, or the time from infection to symptom onset, of COVID-19 has usually been estimated by using data collected through interviews with cases and their contacts. However, this estimation is influenced uncertainty in cases' recall exposure time. We propose a novel method that uses viral load over since hospitalization, hindcasting timing mathematical model for dynamics. As an example, we used reported on 30 hospitalized patients multiple countries (Singapore, China,...
The interplay between coronary hemodynamics and plaque characteristics remains poorly understood.The aim of this study was to compare atherosclerotic phenotypes focal diffuse artery disease (CAD) defined by hemodynamics.This multicenter, prospective, single-arm conducted in 5 countries. Patients with functionally significant lesions based on an invasive fractional flow reserve ≤0.80 were included. Plaque analysis performed using computed tomography angiography optical coherence tomography....
BACKGROUND: Coronary computed tomography angiography (CCTA) is emerging as a valuable tool for noninvasive surveillance of cardiac allograft vasculopathy (CAV) in patients with heart transplant (HTx). We assessed the diagnostic performance comprehensive CCTA-based approach compared invasive reference, which includes coronary angiography, intravascular ultrasound, and fractional flow reserve, detecting CAV. METHODS: This was multicenter prospective study including 37 HTx who underwent CCTA,...
ABSTRACT Anti-hepatitis B virus (HBV) drugs are currently limited to nucleos(t)ide analogs (NAs) and interferons. A challenge of drug development is the identification small molecules that suppress HBV infection from new chemical sources. Here, a fungus-derived secondary metabolite library, we identify structurally novel tricyclic polyketide, named vanitaracin A, which specifically inhibits infection. Vanitaracin inhibited viral entry process with submicromolar 50% inhibitory concentration...
Sodium taurocholate cotransporting polypeptide (NTCP) is expressed at the surface of human hepatocytes and functions as an entry receptor hepatitis B virus (HBV). Recently, we have reported that epidermal growth factor (EGFR) involved in NTCP-mediated viral internalization during cell process. Here, analyzed which function EGFR essential for mediating HBV internalization. In contrast to crucial EGFR-downstream signaling C (HCV), blockade proteins, including mitogen-activated protein kinase...
Background Coronary artery disease (CAD) patterns play an essential role in the decision-making process about revascularization. The pullback pressure gradient (PPG) quantifies CAD as either focal or diffuse based on fractional flow reserve (FFR) pullbacks. objective of this study was to evaluate impact acute percutaneous coronary intervention (PCI) results considered surrogates clinical outcomes. Methods and Results This a prospective, multicenter patients with hemodynamically significant...
Following percutaneous coronary intervention (PCI), optical coherence tomography provides prognosis information. The pullback pressure gradient is a novel index that discriminates focal from diffuse artery disease based on fractional flow reserve pullbacks. We sought to investigate the association between patterns, defined by physiology, and after stent implantation in stable patients undergoing PCI.
Significance Introduction of new anti-hepatitis C virus (HCV) agents, so-called direct-acting antivirals (DAAs), has greatly changed treatment for HCV, and a variety choices anti-HCV drug combinations are available. For better management control this worldwide infectious disease with it is critical to develop method precisely profiling the antiviral efficacy possible combination regimens seek “best treatment” based on scientific evidence. In study, we showed how cell culture data can be...
Viruses hijack and modify host cell functions to maximize viral proliferation. Hepatitis C virus (HCV) reorganizes metabolism produce specialized membrane structures organelles such as double-membrane vesicles enlarged lipid droplets (LDs), thereby enabling replication assembly. However, the molecular bases of these host–HCV interactions are largely unknown. Here, using a chemical screen, we demonstrate that benzamide derivative flutamide reduces capacity infectious HCV. Flutamide disrupted...
Sodium taurocholate cotransporting polypeptide (NTCP) is expressed at the surface of human hepatocytes and functions as an entry receptor hepatitis B virus (HBV). Recently, we have reported that epidermal growth factor (EGFR) involved in NTCP-mediated viral internalization during cell process. Here, analyzed which function EGFR essential for mediating HBV internalization. In contrast to crucial EGFR-downstream signaling C (HCV), blockade proteins, including mitogen-activated protein kinase...
The scientific community is focussed on developing antiviral therapies to mitigate the impacts of ongoing novel coronavirus disease (COVID-19) outbreak. This will be facilitated by improved understanding viral dynamics within infected hosts. Here, using a mathematical model in combination with published load data collected from same specimen (throat / nasal swabs or nasopharyngeal sputum tracheal aspirate), we compare within-host for patients current outbreak analogous MERS-CoV and SARS-CoV...
Coronavirus disease 2019 (COVID-19) has caused serious public health, social, and economic damage worldwide effective drugs that prevent or cure COVID-19 are urgently needed. Approved including Hydroxychloroquine, Remdesivir Interferon were reported to inhibit the infection propagation of severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), however, their clinical efficacies have not yet been well demonstrated. To identify with higher antiviral potency, we screened approved...
Previous work has indicated that a chemoselective resistance controls gastric emptying. By use of meals glucose or oleate, which were shown to empty spontaneously from dogs' stomachs half as fast saline, we sought locate this by studying flow under controlled pressures in various regions the gastrointestinal tract. In intact dogs, outflow oleate rose one-third saline pressure was raised, and increased nutrients unaffected truncal vagotomy pyloroplasty. fistula gastroduodenal linearly with...
Human noroviruses (HuNoVs) are acute viral gastroenteritis pathogens that affect all age groups, yet no approved vaccines and drugs to treat HuNoV infection available. In this study, we screened an antiviral compound library identify compound(s) showing anti-HuNoV activity using a human intestinal enteroid (HIE) culture system in which HuNoVs able replicate reproducibly. Dasabuvir (DSB), has been developed as anti-hepatitis C virus agent, was found inhibit HIEs at micromolar concentrations....